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Human/Mouse Arginase 1/ARG1 PE-conjugated Antibody

Catalog #: IC5868P
40 Citations 1 Review Datasheet / COA / SDS
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IC5868P
Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry.
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Citations (40)
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Reviews (1)
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Human/Mouse Arginase 1/ARG1 PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Arginase 1/ARG1
Met1-Lys322
Accession # P05089
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Arginase 1/ARG1 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of Arginase 1/ARG1 antibody in Hepa 1-6 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Arginase 1/ARG1 in Hepa 1-6 Mouse Cell Line by Flow Cytometry. Hepa 1-6 mouse hepatoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Arginase 1/ARG1

Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

Long Name
Liver-Type Arginase
Entrez Gene IDs
383 (Human); 11846 (Mouse); 29221 (Rat)
Alternate Names
AI; ARG1; Arginase 1; arginase, liver; Arginase-1; EC 3.5.3.1; EC:3.5.3.1; Liver Arginase; Liver-type arginase; PGIF; Type I Arginase

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Citations for Human/Mouse Arginase 1/ARG1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

40 Citations: Showing 1 - 10
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  1. Mitochondrial permeabilization engages NF-kappa B-dependent anti-tumour activity under caspase deficiency
    Authors: Evangelos Giampazolias, Barbara Zunino, Sandeep Dhayade, Florian Bock, Catherine Cloix, Kai Cao et al.
    Nature Cell Biology
  2. 5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interaction
    Authors: Dongsheng Guan, Yingxia Li, Yinglin Cui, Huanghong Zhao, Ning Dong, Kun Wang et al.
    Acta Biochimica et Biophysica Sinica
  3. Advanced Glycation End Products Enhance Macrophages Polarization into M1 Phenotype through Activating RAGE/NF-kappa B Pathway
    Authors: Xian Jin, Tongqing Yao, Zhong’e Zhou, Jian Zhu, Song Zhang, Wei Hu et al.
    BioMed Research International
  4. PDn-3 DPA Pathway Regulates Human Monocyte Differentiation and Macrophage Function Cell Chem Biol May 16 2018 12:00AM [PMID: 29805036] (Flow)
    Authors: Pistorius K, Souza PR, De Matteis R et al.
    Cell Chem Biol. 
  5. AXL is a key factor for cell plasticity and promotes metastasis in pancreatic cancer
    Authors: Du W, Huang H, Wang Z et al.
    Mol Cancer Res
  6. Integrin ?1-rich extracellular vesicles of kidney recruit Fn1+ macrophages to aggravate ischemia-reperfusion-induced inflammation
    Authors: Wang, W;Ren, X;Chen, X;Hong, Q;Cai, G;
    JCI insight
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Heterogeneous ferroptosis susceptibility of macrophages caused by focal iron overload exacerbates rheumatoid arthritis
    Authors: Liu, Y;Luo, X;Chen, Y;Dang, J;Zeng, D;Guo, X;Weng, W;Zhao, J;Shi, X;Chen, J;Dong, B;Zhong, S;Ren, J;Li, Y;Wang, J;Zhang, J;Sun, J;Xu, H;Lu, Y;Brand, D;Zheng, SG;Pan, Y;
    Redox biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Myeloid-intrinsic cell cycle-related kinase drives immunosuppression to promote tumorigenesis
    Authors: Zhou J, Wang H, Shu T et al.
    iScience
  9. SUN1/2 controls macrophage polarization via modulating nuclear size and stiffness
    Authors: Jiao, S;Li, C;Guo, F;Zhang, J;Zhang, H;Cao, Z;Wang, W;Bu, W;Lin, M;Lü, J;Zhou, Z;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. MAGL regulates synovial macrophage polarization vis inhibition of mitophagy in osteoarthritic pain
    Authors: Gu, C;Chen, M;Li, X;Geng, D;Wang, C;
    Molecular medicine reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Single-Cell Transcriptomic Profiles of Lung Pre-Metastatic Niche Reveal Neutrophil and Lymphatic Endothelial Cell Roles in Breast Cancer
    Authors: YC Huang, CY Chang, YY Wu, KL Wu, YM Tsai, HC Lee, EM Tsai, YL Hsu
    Cancers, 2022-12-28;15(1):.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Helminth resistance is mediated by differential activation of recruited monocyte-derived alveolar macrophages and arginine depletion
    Authors: F Chen, DW El-Naccach, JJ Ponessa, A Lemenze, V Espinosa, W Wu, K Lothstein, L Jin, O Antao, JS Weinstein, P Damani-Yok, K Khanna, PJ Murray, A Rivera, MC Siracusa, WC Gause
    Cell Reports, 2022-01-11;38(2):110215.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  13. VEGFR2 activity on myeloid cells mediates immune suppression in the tumor microenvironment
    Authors: Y Zhang, H Huang, M Coleman, A Ziemys, P Gopal, SM Kazmi, RA Brekken
    JCI Insight, 2021-12-08;6(23):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Loss of 15-lipoxygenase disrupts T(reg) differentiation altering their pro-resolving functions
    Authors: Marques RM, Gonzalez-Nunez M, Walker ME et al.
    Cell Death & Differentiation
  15. CCR2 Signaling Promotes Brain Infiltration of Inflammatory Monocytes and Contributes to Neuropathology during Cryptococcal Meningoencephalitis
    Authors: J Xu, A Ganguly, J Zhao, M Ivey, R Lopez, JJ Osterholze, CS Cho, MA Olszewski
    MBio, 2021-07-27;0(0):e0107621.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Ubiquitous Overexpression of Chromatin Remodeling Factor SRG3 Exacerbates Atopic Dermatitis in NC/Nga Mice by Enhancing Th2 Immune Responses
    Authors: SW Lee, HJ Park, J Jeon, YH Park, TC Kim, SH Jeon, RH Seong, L Van Kaer, S Hong
    International Journal of Molecular Sciences, 2021-02-04;22(4):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Optimized protocol for the preparation of single cells from cutaneous wounds for flow cytometric cell sorting and analysis of macrophages
    Authors: S Shakya, K Asosingh, JA Mack, EV Maytin
    MethodsX, 2020-08-08;7(0):101027.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. IL-13 Ameliorates Neuroinflammation and Promotes Functional Recovery after Traumatic Brain Injury
    Authors: W Miao, Y Zhao, Y Huang, D Chen, C Luo, W Su, Y Gao
    J. Immunol., 2020-02-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. STAT6/Arg1 promotes microglia/macrophage efferocytosis and inflammation resolution in stroke mice
    Authors: W Cai, X Dai, J Chen, J Zhao, M Xu, L Zhang, B Yang, W Zhang, M Rocha, T Nakao, J Kofler, Y Shi, RA Stetler, X Hu, J Chen
    JCI Insight, 2019-10-17;4(20):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice
    Authors: A Carestia, HA Mena, CM Olexen, JM Ortiz Wilc, S Negrotto, AE Errasti, RM Gómez, CN Jenne, EA Carrera Si, M Schattner
    Cell Rep, 2019-07-23;28(4):896-908.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Sitravatinib potentiates immune checkpoint blockade in refractory cancer models
    Authors: W Du, H Huang, N Sorrelle, RA Brekken
    JCI Insight, 2018-11-02;3(21):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Concerted IL-25R and IL-4R? signaling drive innate type 2 effector immunity for optimal helminth expulsion
    Authors: KA Smith, S Löser, F Varyani, Y Harcus, HJ McSorley, AN McKenzie, RM Maizels
    Elife, 2018-09-21;7(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Temporal changes in macrophage phenotype after peripheral nerve injury
    Authors: JE Tomlinson, E Žygelyt?, JK Grenier, MG Edwards, J Cheetham
    J Neuroinflammation, 2018-06-15;15(1):185.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype
    Authors: D Chen, J Xie, R Fiskesund, W Dong, X Liang, J Lv, X Jin, J Liu, S Mo, T Zhang, F Cheng, Y Zhou, H Zhang, K Tang, J Ma, Y Liu, B Huang
    Nat Commun, 2018-02-28;9(1):873.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Infection-adapted emergency hematopoiesis promotes visceral leishmaniasis
    Authors: BM Abidin, A Hammami, S Stäger, KM Heinonen
    PLoS Pathog., 2017-08-07;13(8):e1006422.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. IL-4 upregulates cyclooxygenase-1 expression in macrophages
    Authors: AE Shay, BT Diwakar, BJ Guan, V Narayan, JF Urban, KS Prabhu
    J. Biol. Chem., 2017-07-06;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Therapeutic benefits of phosphodiesterase 4B inhibition after traumatic brain injury
    Authors: NM Wilson, ME Gurney, WD Dietrich, CM Atkins
    PLoS ONE, 2017-05-19;12(5):e0178013.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Dysferlinopathy Promotes an Intramuscle Expansion of Macrophages with a Cyto-Destructive Phenotype
    Authors: JH Baek, GM Many, FJ Evesson, VR Kelley
    Am. J. Pathol., 2017-04-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. IL-34 mediates acute kidney injury and worsens subsequent chronic kidney disease.
    Authors: Baek J, Zeng R, Weinmann-Menke J, Valerius M, Wada Y, Ajay A, Colonna M, Kelley V
    J Clin Invest, 2015-06-29;125(8):3198-214.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. Protective Role of STAT6 in Basophil-Dependent Prurigo-like Allergic Skin Inflammation.
    Authors: Hashimoto T, Satoh T, Yokozeki H
    J Immunol, 2015-04-10;194(10):4631-40.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Obesity triggers enhanced MDSC accumulation in murine renal tumors via elevated local production of CCL2.
    Authors: Hale, Malika, Itani, Farah, Buchta, Claire M, Wald, Gal, Bing, Megan, Norian, Lyse A
    PLoS ONE, 2015-03-13;10(3):e0118784.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  32. Anti-melanoma vaccines engineered to simultaneously modulate cytokine priming and silence PD-L1 characterized using myeloid-derived suppressor cells as a readout of therapeutic efficacy.
    Authors: Liechtenstein T, Perez-Janices N, Blanco-Luquin I, Goyvaerts C, Schwarze J, Dufait I, Lanna A, Ridder M, Guerrero-Setas D, Breckpot K, Escors D
    Oncoimmunology, 2014-07-03;3(7):e945378.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Isolation and characterization of immune cells from the tumor microenvironment of genetically engineered pediatric high-grade glioma models using the sleeping beauty transposon system
    Authors: Maria Belen Garcia-Fabiani, Andrea Comba, Padma Kadiyala, Santiago Haase, Felipe Javier Núñez, David Altshuler et al.
    Methods in Enzymology
  34. Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells
    Authors: Tomasz M. Grzywa, Anna Sosnowska, Zuzanna Rydzynska, Michal Lazniewski, Dariusz Plewczynski, Klaudia Klicka et al.
    Communications Biology
  35. Loss of 15-lipoxygenase disrupts T(reg) differentiation altering their pro-resolving functions
    Authors: Marques RM, Gonzalez-Nunez M, Walker ME et al.
    Cell Death & Differentiation
  36. Macrophage Migration Inhibitory Factor (MIF) Is Essential for Type 2 Effector Cell Immunity to an Intestinal Helminth Parasite
    Authors: Kara J. Filbey, Fumi Varyani, Yvonne Harcus, James P. Hewitson, Danielle J. Smyth, Henry J. McSorley et al.
    Frontiers in Immunology
  37. Ultrasound-Targeted Microbubble Destruction Alleviates Immunosuppression Induced by CD71+ Erythroid Progenitor Cells and Promotes PDL-1 Blockade Immunotherapy in the Lewis Lung Cancer Model
    Authors: Xi Tan, Cuo Yi, Yi Zhang, Najiao Tang, Yali Xu, Zheng Liu
    Frontiers in Oncology
  38. Myeloid-intrinsic cell cycle-related kinase drives immunosuppression to promote tumorigenesis
    Authors: Zhou J, Wang H, Shu T et al.
    iScience
  39. Vagal Regulation of Group 3 Innate Lymphoid Cells and the Immunoresolvent PCTR1 Controls Infection Resolution
    Authors: Jesmond Dalli, Romain A. Colas, Hildur Arnardottir, Charles N. Serhan
    Immunity
  40. IL9 Polarizes Macrophages to M1 and Induces the Infiltration of Antitumor Immune Cells via MIP-1 and CXCR3 Chemokines
    Authors: Van Anh Do-Thi, Sang Min Park, Song Mi Park, Hye Jin Jeong, Geunyoung Cho, Hyun-Jung An et al.
    Cancer Research Communications

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Human/Mouse Arginase 1/ARG1 PE-conjugated Antibody
By Anonymous on 05/24/2017
Application: Flow Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line Species: Mouse