Human/Mouse ASAM Antibody

Catalog #: AF7356 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7356
AF7356-SP

Detection of Mouse ASAM by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse ASAM Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse recombinant ASAM in direct ELISAs. Detects human and mouse ASAM in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse ASAM
Thr18-Ala234
Accession # Q8R373

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse ASAM by Western Blot. Western blot shows lysates of mouse adipose (uterus) tissue and mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ASAM at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of ASAM in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Flow Cytometry

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Detection of ASAM in T98G Human Cell Line by Flow Cytometry. T98G human glioblastoma cell line was stained with Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASAM

ASAM (Adipocyte-specific adhesion molecule; also ACAM, CLMP, Asp5 and CXADR-like protein) is a 44-48 kDa member of the CTX family of molecules. It is expressed on adipocytes as well as select epithelial cells such as respiratory and intestinal epithelium, keratinocytes and Sertoli cells. ASAM appears to contribute to tight junction formation, and may promote the transition of preadipocytes to adipocytes. Mature mouse ASAM is a 356 amino acid (aa) type I transmembrane glycoprotein. It contains a 217 aa extracellular region (aa 18-234) that is characterized by the presence of two C2 type Ig-like domains (aa 18-126 and 134-223), and possesses a C-terminal 118 aa cytoplasmic domain. The extracellular region of mouse ASAM (aa 18-234) shares 97% and 100% aa sequence identity with human and rat ASAM, respectively.

Long Name

Adipocyte-specific Adhesion Molecule

Entrez Gene IDs

79827 (Human); 71566 (Mouse); 286939 (Rat)

Alternate Names

ACAM; ACAMCoxsackie- and adenovirus receptor-like membrane protein; Adipocyte adhesion molecule; adipocyte-specific adhesion molecule; ASAM; ASAMCAR-like membrane protein; Asp5; CLMP; CXADR-like membrane protein; FLJ22415