Human/Mouse ATG4A Antibody

Catalog #: MAB4324 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4324
MAB4324-SP

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Human/Mouse ATG4A Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human ATG4A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human ATG4B is observed.

Source

Monoclonal Mouse IgG_2B Clone # 514914

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ATG4A isoform 1
Glu2-Val398
Accession # Q8WYN0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human ATG4A

Immunoprecipitation

25 µg/mL

Cell lysates spiked with Recombinant Human ATG4A, see our available Western blot detection antibodies

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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ATG4A in RAW 264.7 Mouse Cell Line. ATG4A was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human ATG4A Monoclonal Antibody (Catalog # MAB4324) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATG4A

Autophagy related protein 4 homolog A (ATG4A), also known as autophagin 2, is a 50 kDa cytosolic cysteine protease mostly found in skeletal muscle and brain. It belongs to the autophagin protein family which is involved in autophagy, a biological process in which endogenous proteins and damaged organelles are destroyed. Human ATG4A shares 100%, 94% and 93% identity with that of chimpanzee, dog and mouse.

Long Name

ATG4 Autophagy Related 4 Homolog A

Entrez Gene IDs

115201 (Human); 666468 (Mouse)

Alternate Names

APG4 autophagy 4 homolog A (S. cerevisiae); Apg4a; ATG4 autophagy related 4 homolog A (S. cerevisiae); ATG4A; Atg4al; Autl2; AUT-like 2 cysteine endopeptidase; AUT-like 2, cysteine endopeptidase (S. cerevisiae); Autophagin 2; autophagin-2; cysteine endopeptidase; cysteine protease ATG4A; EC 3.4.22; EC 3.4.22.-; hAPG4A