Human/Mouse BCAP Antibody

Catalog #: MAB4857 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4857
MAB4857-SP

Detection of Human BCAP by Western Blot.

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Human/Mouse BCAP Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human and mouse BCAP in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 501813

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human BCAP
Met462-Leu650
Accession # Q6ZUJ8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

RL human non-Hodgkin's lymphoma B cell line and Daudi human Burkitt's lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human BCAP by Western Blot. Western Blot shows lysates of RL human non-Hodgkin's lymphoma B cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human/Mouse BCAP Monoclonal Antibody (Catalog # MAB4857) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for BCAP at approximately 80 kDa and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCAP

BCAP (B cell adaptor for phosphoinositide 3-kinase (PI3K)), also known as PI3KAP1, participates in linking the B cell antigen receptor (BCR) with the PI3K pathway. Tyrosine phosphorylation of BCAP by BCR-associated protein tyrosine kinases, such as Syk and Btk, generates binding sites for the p85 subunit of PI3K, resulting in activation of the PI3K pathway. Human and mouse BCAP contain 3 YXXM SH2 binding motifs for interaction with PI3K. BCAP null mice have deficient immune function, with B cells that exhibit enhanced apoptosis.

Long Name

B Cell Adaptor Protein

Entrez Gene IDs

118788 (Human); 83490 (Mouse); 294048 (Rat)

Alternate Names

1810044J04Rik; B cell adaptor protein; BCAP; BCAPB-cell adapter for phosphoinositide 3-kinase; B-cell phosphoinositide 3-kinase adapter protein 1; FLJ35564; phosphoinositide 3-kinase adapter protein 1; phosphoinositide-3-kinase adaptor protein 1; Pik3ap1; RP11-34E5.3