Human/Mouse Bcl-w Antibody

Catalog #: MAB8241 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8241
MAB8241-SP

Bcl-w Inhibition of BID-induced Release of Cytochrome c and Neutralization by BCL-w Antibody.

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Human/Mouse Bcl-w Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Bcl‑w. Neutralizes recombinant human and mouse Bcl-w, but not recombinant human Bcl‑2 or Bcl‑x.

Source

Monoclonal Mouse IgG₁ Clone # 90336

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Bcl-w
Met1-Thr172
Accession # Q92843

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize Recombinant Human Bcl-w (Catalog # 824-BW) inhibition of caspase-8-cleaved Recombinant Human BID (Catalog # 882-B8) induced release of cytochrome c from isolated mouse liver mitochondria using the Rat/Mouse Cytochrome c Quantikine ELISA Kit (Catalog # MCTC0).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Bcl-w Inhibition of BID-induced Release of Cytochrome c and Neutralization by BCL-w Antibody. Cytochrome c released from enriched mouse liver mitochondria solubilized with Triton^®X-100 was quantified with the Rat/Mouse Cytochrome c ELISA (Catalog # MCTC0).Untreated: Release of Cytochrome c from untreated mitochondriaBID: Recombinant Human BID (Caspase-8-cleaved) (882-B8) induced release of Cytochrome c from mitochondriaBID + Bcl-w: Recombinant Human Bcl-w (824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (882-B8) induced release of Cytochrome c from mitochondriaBID + Bcl-w + MAB8241: Mouse Anti-Human/Mouse Bcl-w Monoclonal Antibody (Catalog # MAB8241) neutralized Recombinant Human Bcl-w (824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (882-B8) induced release of Cytochrome c from mitochondria

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-w

Bcl-w is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

References