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Human/Mouse Bcl-w Antibody

Catalog #: AF824
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF824
AF824-SP
Detection of Human and Mouse Bcl‑w by Western Blot.
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Citations (2)
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Human/Mouse Bcl-w Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Bcl-w in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
KLH-coupled human/mouse Bcl-w synthetic peptide
Gly32-Asp51
Accession # Q92843
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Bcl-w antibody by Western Blot. View Larger

Detection of Human and Mouse Bcl‑w by Western Blot. Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1.2 µg/mL Rabbit Anti-Human/Mouse Bcl-w Antigen Affinity-purified Polyclonal Antibody (Catalog # AF824) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Bcl-w Minus C-Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-w

Bcl-w, also called Bcl-2 like protein 2, is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

References
  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Kroemer, G. (1997) Nature Med. 3:614.
  3. Ross, J.A., et al. (1998) Nat. Genet. 18:251.
  4. Print, C.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12323.
  5. Yan, W. et al. (2000) Mol. Endocrin. 14:682.
Long Name
B Cell Lymphoma/Leukemia w
Entrez Gene IDs
599 (Human); 12050 (Mouse)
Alternate Names
Apoptosis regulator Bcl-W; BCL2L2; Bcl2-L-2; BCL2-like 2; Bclw; Bcl-w; BCLWbcl-2-like protein 2; KIAA0271BCL2-L-2

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Citations for Human/Mouse Bcl-w Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Sensitivity to splicing modulation of BCL2 family genes defines cancer therapeutic strategies for splicing modulators
    Authors: D Aird, T Teng, CL Huang, E Pazolli, D Banka, K Cheung-Ong, C Eifert, C Furman, ZJ Wu, M Seiler, S Buonamici, P Fekkes, C Karr, J Palacino, E Park, PG Smith, L Yu, Y Mizui, M Warmuth, A Chicas, L Corson, P Zhu
    Nat Commun, 2019-01-11;10(1):137.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
    Authors: Jayanthi S, åÏctÐs, Deng X, Bordelon M, McCoy MT, Cadet JL
    2001-08-01;15(10):1745-52.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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