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Human/Mouse BID Biotinylated Antibody

Catalog #: BAF860
2 Citations Datasheet / COA / SDS
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BAF860
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Citations (2)
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Human/Mouse BID Biotinylated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse BID in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse BID
Met1-Asp195
Accession # AAC71064
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse BID (Catalog # 860-MB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BID

BID is a 195 amino acid member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1). BID is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells BID is cytosolic. In response to Fas ligand or TNF, BID is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane (2, 3). Cleavage of BID by caspase-8 generates a new N-terminal that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target BID to the mitochondria (4). BID may then interact with another pro-apoptotic Bcl-2 family member Bak (5). Interaction of BID with Bak causes altered mitochondrial membrane permeability. A 9‑13 amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the BID interaction with other Bcl-2 family members. BID is neutralized by binding to the anti-apoptotic member Bcl-xL.

References
  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Luo, X., et al. (1998) Cell 94:481.
  3. Li, H. et al. (1998) Cell 94:491.
  4. Zha, J. et al. (2000) Science 290:1761.
  5. Wei, M.C. et al. (2000) Genes Dev. 14:2060.
Long Name
BH3 Interacting Domain Death Agonist
Entrez Gene IDs
637 (Human); 12122 (Mouse)
Alternate Names
apoptic death agonist; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; BID; desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p22 BID; tbid

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Citations for Human/Mouse BID Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Endoplasmic reticulum stress contributes to Helicobacter pylori VacA-induced apoptosis.
    Authors: Akazawa, Yuko, Isomoto, Hajime, Matsushima, Kayoko, Kanda, Tsutomu, Minami, Hitomi, Yamaghchi, Naoyuki, Taura, Naota, Shiozawa, Ken, Ohnita, Ken, Takeshima, Fuminao, Nakano, Masayuki, Moss, Joel, Hirayama, Toshiya, Nakao, Kazuhiko
    PLoS ONE, 2013-12-13;8(12):e82322.
    Species: Human
    Sample Types: Cell Lysates
  2. BID Binds to Replication Protein A and Stimulates ATR Function following Replicative Stress.
    Authors: Liu Y, Vaithiyalingam S, Shi Q
    Mol. Cell. Biol., 2011-08-22;31(21):4298-309.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunoprecipitation

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