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Human/Mouse BMP-10 Antibody

Catalog #: MAB2926
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2926-SP
MAB2926-500
MAB2926-100
Alkaline Phosphatase Production Induced by BMP‑10 and Neutralization by Human BMP‑10 Antibody.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse BMP-10 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human BMP-10 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BMP-1.1, 2, 3, 3b, 4, 5, 6, 7, 9, 15, or the pro region of rhBMP-10 is observed. In direct ELISAs, approximately 90% cross-reactivity with recombinant mouse BMP-10 is observed.
Source
Monoclonal Mouse IgG2A Clone # 462732
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human BMP-10
Asn317-Arg424
Accession # O95393
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human BMP‑10 (Catalog # 2926-BP)
under non-reducing conditions only
Neutralization
Measured by its ability to neutralize BMP‑10-induced alkaline phosphatase production in the MC3T3‑E1 mouse preosteoblast cell line. The Neutralization Dose (ND50) is typically 0.15-0.9 µg/mL in the presence of 100 ng/mL Recombinant Human BMP‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Alkaline Phosphatase Production Induced by BMP‑10 and Neutralization by Human BMP‑10 Antibody. View Larger

Alkaline Phosphatase Production Induced by BMP‑10 and Neutralization by Human BMP‑10 Antibody. Recombinant Human BMP-10 (Catalog # 2926-BP) induces alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-10 (100 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP-10 Monoclonal Antibody (Catalog # MAB2926). The ND50 is typically 0.15-0.9 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-10

Bone morphogenetic protein 10 (BMP-10) is a TGF-beta superfamily member whose expression is restricted to the developing and postnatal heart. It is synthesized as a 424 amino acid (aa) pre-proprotein with a putative 21 aa signal peptide, a 295 aa propeptide, and a 108 aa mature protein. Mature BMP-10 is secreted as a 25 kDa disulfide-linked homodimer. Mature human BMP-10 shares 98% aa sequence identity with mature mouse, and rat BMP-10.

Long Name
Bone Morphogenetic Protein 10
Entrez Gene IDs
27302 (Human); 12154 (Mouse)
Alternate Names
BMP10; BMP-10; bone morphogenetic protein 10; MGC126783

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Citations for Human/Mouse BMP-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. CDK6-mediated endothelial cell cycle acceleration drives arteriovenous malformations in hereditary hemorrhagic telangiectasia
    Authors: Dinakaran, S;Zhao, H;Tang, Y;Wang, Z;Ruiz, S;Nomura-Kitabayashi, A;Blanc, L;Faughnan, ME;Marambaud, P;
    bioRxiv : the preprint server for biology
    Species: Transgenic Mouse, Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Crystal structures of BMPRII extracellular domain in binary and ternary receptor complexes with BMP10
    Authors: J Guo, B Liu, M Thorikay, M Yu, X Li, Z Tong, RM Salmon, RJ Read, P Ten Dijke, NW Morrell, W Li
    Nature Communications, 2022-05-03;13(1):2395.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: ELISA Capture
  3. Homozygous GDF2 nonsense mutations result in a loss of circulating BMP9 and BMP10 and are associated with either PAH or an “HHT‐like” syndrome in children
    Authors: Joshua Hodgson, Lidia Ruiz‐Llorente, Jamie McDonald, Oliver Quarrell, Kelechi Ugonna, James Bentham et al.
    Molecular Genetics & Genomic Medicine
  4. Reduced circulating BMP10 and BMP9 and elevated endoglin are associated with disease severity, decompensation and pulmonary vascular syndromes in patients with cirrhosis
    Authors: NE Owen, GJ Alexander, S Sen, K Bunclark, G Polwarth, J Pepke-Zaba, AP Davenport, NW Morrell, PD Upton
    EBioMedicine, 2020-05-23;56(0):102794.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA (detection)
  5. A heterodimer formed by bone morphogenetic protein 9 (BMP9) and BMP10 provides most BMP biological activity in plasma
    Authors: E Tillet, M Ouarné, A Desroches-, C Mallet, M Subileau, R Didier, A Lioutsko, G Belthier, JJ Feige, S Bailly
    J. Biol. Chem., 2018-05-22;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Combined effects of bone morphogenetic protein 10 and crossveinless-2 on cardiomyocyte differentiation in mouse adipocyte-derived stem cells
    Authors: Medet Jumabay, Jiayinaguli Zhumabai, Nurlan Mansurov, Katharine C. Niklason, Pierre J. Guihard, Mark R. Cubberly et al.
    Journal of Cellular Physiology
  7. A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10
    Sci Rep, 2016-11-22;5(0):37366.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  8. BMP9 and BMP10 are necessary for proper closure of the ductus arteriosus.
    Authors: Levet S, Ouarne M, Ciais D, Coutton C, Subileau M, Mallet C, Ricard N, Bidart M, Debillon T, Faravelli F, Rooryck C, Feige J, Tillet E, Bailly S
    Proc Natl Acad Sci U S A, 2015-06-08;112(25):E3207-15.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  9. BMP9 and BMP10 are critical for postnatal retinal vascular remodeling.
    Blood, 2012-05-07;119(25):6162-71.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. Systematic analysis of gene expression differences between left and right atria in different mouse strains and in human atrial tissue.
    Authors: Kahr PC, Piccini I, Fabritz L, Greber B, Scholer H, Scheld HH, Hoffmeier A, Brown NA, Kirchhof P
    PLoS ONE, 2011-10-19;6(10):e26389.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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