Human/Mouse BMP-8b Antibody Summary
Thr261-His399
Accession # NP_031585
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse BMP‑8b by Western Blot. Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse BMP-8b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6305) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pro-BMP-8b at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-8b
BMP-8b (Bone morphogenetic protein 8b; also OP-3) is a 16 kDa predicted mature and 45 kDa precurser member of the Gbb-60A class of the BMP family of molecules. In mouse, precursors to sperm and oocytes arise from the proximal epiblast around day E7.5 (days 18-20 in human). This lineage commitment is a response to BMP-4 signaling, which is promoted by the repressive action of BMP-8b on the anterior visceral endoderm, a source of BMP-4 antagonism. Mouse BMP-8b preproprecursor is 399 amino acids (aa) in length. It contains a 19 aa signal sequence, a 241 aa prosegment, and a 139 aa mature region. An interchain disulfide bond occurs at Cys363, generating a homodimer. Over aa 261-399, mouse BMP-8b shares 74% aa identity with mouse BMP-8a, and 69% and 93% aa identity with human and rat BMP-8b, respectively.
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The figures was used to stain with IF BMP8B in human and murine FFPE liver slides with IF. Details of the figures (Fig 1 and Extended Figure 1) and experimental conditions can be found in the published paper: https://www.nature.com/articles/s42255-020-0214-9 where it is shown that BMP8B is expressed in acute and chronic liver disease by hepatocytes (CK18+/Alb+) and Activated Stellate Cells (aSMA+) both in humans and mice.
