Human/Mouse BMP-8b Antibody

Catalog # Availability Size / Price Qty
AF6305
AF6305-SP

Save on RUO reagents! Up to 30% off + FREE shipping. See Details.

Detection of Mouse BMP‑8b by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews (1)

Human/Mouse BMP-8b Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse BMP-8b in direct ELISAs and human and mouse in Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) BMP-8a and less than 1% cross‑reactivity with recombinant human (rh) BMP‑3b, rhBMP‑10, and rm BMP‑10 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse BMP-8b
Thr261-His399
Accession # NP_031585
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse BMP-8b antibody by Western Blot. View Larger

Detection of Mouse BMP‑8b by Western Blot. Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse BMP-8b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6305) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pro-BMP-8b at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-8b

BMP-8b (Bone morphogenetic protein 8b; also OP-3) is a 16 kDa predicted mature and 45 kDa precurser member of the Gbb-60A class of the BMP family of molecules. In mouse, precursors to sperm and oocytes arise from the proximal epiblast around day E7.5 (days 18-20 in human). This lineage commitment is a response to BMP-4 signaling, which is promoted by the repressive action of BMP-8b on the anterior visceral endoderm, a source of BMP-4 antagonism. Mouse BMP-8b preproprecursor is 399 amino acids (aa) in length. It contains a 19 aa signal sequence, a 241 aa prosegment, and a 139 aa mature region. An interchain disulfide bond occurs at Cys363, generating a homodimer. Over aa 261-399, mouse BMP-8b shares 74% aa identity with mouse BMP-8a, and 69% and 93% aa identity with human and rat BMP-8b, respectively.

Long Name
Bone Morphogenetic Protein 8b
Entrez Gene IDs
656 (Human); 12164 (Mouse)
Alternate Names
BMP-8; BMP-8b; bone morphogenetic protein 8b; MGC131757; OP-3

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Mouse BMP-8b Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse BMP-8b Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse BMP-8b Antibody
By Anonymous on 10/05/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Liver tissue Species: Human and Mouse

The figures was used to stain with IF BMP8B in human and murine FFPE liver slides with IF. Details of the figures (Fig 1 and Extended Figure 1) and experimental conditions can be found in the published paper: https://www.nature.com/articles/s42255-020-0214-9 where it is shown that BMP8B is expressed in acute and chronic liver disease by hepatocytes (CK18+/Alb+) and Activated Stellate Cells (aSMA+) both in humans and mice.