Human/Mouse BMP-9 Antibody

Catalog # Availability Size / Price Qty
AF3209
AF3209-SP
Alkaline Phosphatase Production Induced by BMP‑9 and Neutralization by Human BMP‑9 Antibody.
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Product Details
Citations (4)
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Human/Mouse BMP-9 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human BMP-9 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse BMP‑9 is observed. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) BMP-2, -3, -4, -5, -7, -8, and
rhBMP‑10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human BMP-9
Ser320-Arg429
Accession # Q9UK05
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize BMP‑9-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 0.005-0.1 µg/mL in the presence of 2 ng/mL Recombinant Human BMP‑9.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Alkaline Phosphatase Production Induced by BMP‑9 and Neutralization by Human BMP‑9 Antibody. View Larger

Alkaline Phosphatase Production Induced by BMP‑9 and Neutralization by Human BMP‑9 Antibody. Recombinant Human BMP-9 (Catalog # 3209-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phospha-tase production elicited by Recombinant Human BMP-9 (2 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human BMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3209). The ND50 is typically 0.005-0.1 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-9

Human BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK-1 (4-6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8-10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).

References
  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
  3. Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136.
  4. Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111.
  5. Song, J.J. et al. (1995) Endocrinology 136:4293.
  6. Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937.
  7. Chen, C. et al. (2003) Nat. Biotechnol. 21:294.
  8. Lopez-Coviella, I. et al. (2000) Science 289:313.
  9. Lopez-Coviella, I. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6984.
  10. Lopez-Coviella, I. et al. (2002) J. Physiol. Paris 96:53.
  11. Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275.
  12. Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611.
  13. Ploemacher, R.E. et al. (1999) Leukemia 13:428.
Long Name
Bone Morphogenetic Protein 9
Entrez Gene IDs
2658 (Human); 12165 (Mouse)
Alternate Names
BMP9; BMP-9; BMP9BMP-9Bone morphogenetic protein 9; GDF2; GDF-2; growth differentiation factor 2; growth/differentiation factor 2

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Citations for Human/Mouse BMP-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. ALK1 Signaling Inhibits Angiogenesis by Cooperating with the Notch Pathway
    Authors: Bruno Larrivée, Claudia Prahst, Emma Gordon, Raquel del Toro, Thomas Mathivet, Antonio Duarte et al.
    Developmental Cell
  2. Adenovirus?mediated overexpression of bone morphogenetic protein?9 promotes methionine choline deficiency?induced non?alcoholic steatohepatitis in non?obese mice
    Authors: Q Li, B Liu, K Breitkopf-, H Weng, Q Jiang, P Dong, S Dooley, K Xu, H Ding
    Mol Med Rep, 2019-07-19;20(3):2743-2753.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Abcc6 deficiency causes increased infarct size and apoptosis in a mouse cardiac ischemia-reperfusion (I/R) model
    Authors: Imran N. Mungrue, Peng Zhao, Yucheng Yao, Haijin Meng, Christoph Rau, Jocelyn V. Havel et al.
    Arteriosclerosis, Thrombosis, and Vascular Biology
  4. Regulation of Bone Morphogenetic Protein 9 (BMP9) by Redox-dependent Proteolysis*
    Authors: Zhenquan Wei, Richard M. Salmon, Paul D. Upton, Nicholas W. Morrell, Wei Li
    Journal of Biological Chemistry

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