Home / Insulin / Human/Mouse/Bovine Insulin Antibody

Human/Mouse/Bovine Insulin Antibody

Catalog #: MAB1417
24 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1417
MAB1417-SP
Insulin in beta TC‑6 Mouse Cell Line.
2 Images
Product Details
Citations (24)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Bovine Insulin Antibody Summary

Species Reactivity
Human, Mouse, Bovine
Specificity
Detects bovine, human, and mouse insulin.
Source
Monoclonal Rat IgG2A Clone # 182410
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Insulin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
0.5-25 µg/mL
Immersion fixed paraffin-embedded sections of human pancreas
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
beta TC‑6 mouse beta cell insulinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Insulin antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Insulin in beta TC‑6 Mouse Cell Line. Insulin was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry View Larger

Insulin in Human Pancreas. Insulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Insulin

Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.

Entrez Gene IDs
3630 (Human); 16333 (Mouse); 397415 (Porcine); 280829 (Bovine); 483665 (Canine)
Alternate Names
IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human/Mouse/Bovine Insulin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Mapping and targeted viral activation of pancreatic nerves in mice reveal their roles in the regulation of glucose metabolism
    Authors: M. Jimenez-Gonzalez, R. Li, L. E. Pomeranz, A. Alvarsson, R. Marongiu, R. F. Hampton et al.
    Nature Biomedical Engineering
  2. IL18 signaling causes islet beta cell development and insulin secretion via different receptors on acinar and beta cells
    Authors: Xian Zhang, Songyuan Luo, Minjie Wang, Qin Huang, Wenqian Fang, Jie Li et al.
    Developmental Cell
  3. Generation of Islet-like Cell Aggregates from Human Adipose Tissue-derived Stem Cells by Lentiviral Overexpression of PDX-1
    Authors: M. Bahrebar, M. Soleimani, M. H. Karimi, A. Vahdati, R. Yaghobi
    Int J Organ Transplant Med
  4. Virgin beta -Cells at the Neogenic Niche Proliferate Normally and Mature Slowly
    Authors: Sharon Lee, Jing Zhang, Supraja Saravanakumar, Marcus F. Flisher, David R. Grimm, Talitha van der Meulen et al.
    Diabetes
  5. Ciliary ARL13B prevents obesity in mice
    Authors: Terry, TT;Gigante, ED;Alexandre, CM;Brewer, KM;Engle, SE;Yue, X;Berbari, NF;Vaisse, C;Caspary, T;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types:
    Applications: IHC
  6. STAT3 suppression and beta-cell ablation enhance alpha-to-beta reprogramming mediated by Pdx1
    Authors: Y Wakabayash, T Miyatsuka, M Miura, M Himuro, T Taguchi, H Iida, Y Nishida, Y Fujitani, H Watada
    Scientific Reports, 2022-12-10;12(1):21419.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Morus alba L. (Sangzhi) Alkaloids Promote Insulin Secretion, Restore Diabetic beta-Cell Function by Preventing Dedifferentiation and Apoptosis
    Authors: L Lei, Y Huan, Q Liu, C Li, H Cao, W Ji, X Gao, Y Fu, P Li, R Zhang, Z Abliz, Y Liu, S Liu, Z Shen
    Frontiers in Pharmacology, 2022-03-03;13(0):841981.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Time-restricted feeding prevents deleterious metabolic effects of circadian disruption through epigenetic control of beta cell function
    Authors: MR Brown, SK Sen, A Mazzone, TK Her, Y Xiong, JH Lee, N Javeed, CS Colwell, K Rakshit, NK LeBrasseur, A Gaspar-Mai, T Ordog, AV Matveyenko
    Science Advances, 2021-12-15;7(51):eabg6856.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. Tet2 Controls the Responses of beta cells to Inflammation in Autoimmune Diabetes
    Authors: J Rui, S Deng, AL Perdigoto, G Ponath, R Kursawe, N Lawlor, T Sumida, M Levine-Rit, ML Stitzel, D Pitt, J Lu, KC Herold
    Nature Communications, 2021-08-20;12(1):5074.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Glucagon-Like Peptide-1 Receptor Agonist Prevented the Progression of Hepatocellular Carcinoma in a Mouse Model of Nonalcoholic Steatohepatitis
    Authors: M Kojima, H Takahashi, T Kuwashiro, K Tanaka, H Mori, I Ozaki, Y Kitajima, Y Matsuda, K Ashida, Y Eguchi, K Anzai
    Int J Mol Sci, 2020-08-10;21(16):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Pancreatic islets seeded in a novel bioscaffold forms an organoid to rescue insulin production and reverse hyperglycemia in models of type 1 diabetes
    Authors: DM Elizondo, NZD Brandy, RLL da Silva, TR de Moura, J Ali, D Yang, MW Lipscomb
    Sci Rep, 2020-03-09;10(1):4362.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Reversal of Hyperglycemia and Suppression of Type 1 Diabetes in the NOD Mouse with Apoptotic DNA Immunotherapy� (ADi�), ADi-100
    Authors: DG Alleva, M Rezaee, L Yip, G Ren, J Rosenberg, W Concepcion, A Escher, S Shabahang, AS Thakor
    Biomedicines, 2020-03-04;8(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. VEGF-B ablation in pancreatic beta-cells upregulates insulin expression without affecting glucose homeostasis or islet lipid uptake
    Authors: FC Ning, N Jensen, J Mi, W Lindström, M Balan, L Muhl, U Eriksson, I Nilsson, D Nyqvist
    Sci Rep, 2020-01-22;10(1):923.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Single-Cell Heterogeneity Analysis and CRISPR Screen Identify Key ?-Cell-Specific Disease Genes
    Authors: Z Fang, C Weng, H Li, R Tao, W Mai, X Liu, L Lu, S Lai, Q Duan, C Alvarez, P Arvan, A Wynshaw-Bo, Y Li, Y Pei, F Jin, Y Li
    Cell Rep, 2019-03-12;26(11):3132-3144.e7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Macrophage contact dependent and independent TLR4 mechanisms induce beta-cell dysfunction and apoptosis in a mouse model of type 2 diabetes.
    Authors: Cucak H, Mayer C, Tonnesen M, Thomsen L, Grunnet L, Rosendahl A
    PLoS ONE, 2014-03-03;9(3):e90685.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Accumulation of M1-like macrophages in type 2 diabetic islets is followed by a systemic shift in macrophage polarization.
    Authors: Cucak H, Grunnet L, Rosendahl A
    J Leukoc Biol, 2013-09-05;95(1):149-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Targeting VEGF-B as a novel treatment for insulin resistance and type 2 diabetes.
    Authors: Hagberg C, Mehlem A, Falkevall A, Muhl L, Fam B, Ortsater H, Scotney P, Nyqvist D, Samen E, Lu L, Stone-Elander S, Proietto J, Andrikopoulos S, Sjoholm A, Nash A, Eriksson U
    Nature, 2012-09-26;490(7420):426-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Beta-cell specific deletion of Dicer1 leads to defective insulin secretion and diabetes mellitus.
    Authors: Kalis M, Bolmeson C, Esguerra JL, Gupta S, Edlund A, Tormo-Badia N, Speidel D, Holmberg D, Mayans S, Khoo NK, Wendt A, Eliasson L, Cilio CM
    PLoS ONE, 2011-12-27;6(12):e29166.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  19. Conversion of immortal liver progenitor cells into pancreatic endocrine progenitor cells by persistent expression of Pdx-1.
    Authors: Jin CX, Li WL, Xu F, Geng ZH, He ZY, Su J, Tao XR, Ding XY, Wang X, Hu YP
    J. Cell. Biochem., 2008-05-01;104(1):224-36.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  20. Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress.
    Authors: Dowling P, O'Driscoll L, O'Sullivan F, Dowd A, Henry M, Jeppesen PB, Meleady P, Clynes M
    Proteomics, 2006-12-01;6(24):6578-87.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  21. The development of a GPR44 targeting radioligand [11C]AZ12204657 for in vivo assessment of beta cell mass
    Authors: Mahabuba Jahan, Peter Johnström, Ram K. Selvaraju, Marie Svedberg, Maria Sörhede Winzell, Jenny Bernström et al.
    EJNMMI Research
  22. SHBG141–161 Domain-Peptide Stimulates GPRC6A-Mediated Response in Leydig and beta -Langerhans cell lines
    Authors: Luca De Toni, Diego Guidolin, Vincenzo De Filippis, Daniele Peterle, Maria Santa Rocca, Andrea Di Nisio et al.
    Scientific Reports
  23. Stachyose Improves the Effects of Berberine on Glucose Metabolism by Regulating Intestinal Microbiota and Short-Chain Fatty Acids in Spontaneous Type 2 Diabetic KKAy Mice
    Authors: Hui Cao, Caina Li, Lei Lei, Xing Wang, Shuainan Liu, Quan Liu et al.
    Frontiers in Pharmacology
  24. Perinatal exposure to high dietary advanced glycation end products in transgenic NOD8.3 mice leads to pancreatic beta cell dysfunction
    Authors: Danielle J. Borg, Felicia Y. T. Yap, Sahar Keshvari, David G. Simmons, Linda A. Gallo, Amelia K. Fotheringham et al.
    Islets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse/Bovine Insulin Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Bovine Insulin Antibody and earn rewards!

Have you used Human/Mouse/Bovine Insulin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review