Human/Mouse/Bovine Insulin APC-conjugated Antibody

Catalog #: IC1417A
8 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
IC1417A

Detection of Insulin in beta TC‑6 Mouse Cell Line by Flow Cytometry.

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All Insulin Antibodies

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Citations (8)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Bovine Insulin APC-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Bovine

Specificity

Detects bovine, human, and mouse insulin.

Source

Monoclonal Rat IgG_2A Clone # 182410

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Insulin

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Insulin antibody in beta TC-6 Mouse Cell Line antibody by Flow Cytometry.

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Detection of Insulin in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Rat Anti-Human/Mouse/Bovine Insulin APC-conjugated Monoclonal Antibody (Catalog # IC1417A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Insulin

Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.

Entrez Gene IDs

3630 (Human); 16333 (Mouse); 397415 (Porcine); 280829 (Bovine); 483665 (Canine)

Alternate Names

IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human/Mouse/Bovine Insulin APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Differentially expressed microRNAs during the differentiation of muscle-derived stem cells into insulin-producing cells, a promoting role of microRNA-708-5p/STK4 axis
Authors: Y Ren, X Wang, H Liang, Y Ma
PLoS ONE, 2022-04-08;17(4):e0266609.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry
RNA aptamers specific for transmembrane p24 trafficking protein 6 and Clusterin for the targeted delivery of imaging reagents and RNA therapeutics to human beta cells
Authors: D Van Simaey, A De La Fuen, S Zilio, A Zoso, V Kuznetsova, O Alcazar, P Buchwald, A Grilli, J Caroli, S Bicciato, P Serafini
Nature Communications, 2022-04-05;13(1):1815.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
The IGFBP3/TMEM219 pathway regulates beta cell homeostasis
Authors: F D'Addio, A Maestroni, E Assi, M Ben Nasr, G Amabile, V Usuelli, C Loretelli, F Bertuzzi, B Antonioli, F Cardarelli, B El Essawy, A Solini, IC Gerling, C Bianchi, G Becchi, S Mazzucchel, D Corradi, GP Fadini, D Foschi, JF Markmann, E Orsi, J Škrha, MG Camboni, R Abdi, AM James Shap, F Folli, J Ludvigsson, S Del Prato, G Zuccotti, P Fiorina
Nature Communications, 2022-02-03;13(1):684.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Potential Protection Against Type 2 Diabetes in Obesity Through Lower CD36 Expression and Improved Exocytosis in beta -Cells
Authors: Mototsugu Nagao, Jonathan L.S. Esguerra, Akira Asai, Jones K. Ofori, Anna Edlund, Anna Wendt et al.
Diabetes
Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress
Authors: C Merriman, D Fu
J. Biol. Chem., 2019-10-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
microRNA-690 regulates induced pluripotent stem cells (iPSCs) differentiation into insulin-producing cells by targeting Sox9
Authors: Y Xu, Y Huang, Y Guo, Y Xiong, S Zhu, L Xu, J Lu, X Li, J Wan, Y Lu, Z Wang
Stem Cell Res Ther, 2019-02-15;10(1):59.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Genetic predisposition for beta cell fragility underlies type 1 and type 2 diabetes
Authors: J Dooley, L Tian, S Schonefeld, V Delghingar, JE Garcia-Per, E Pasciuto, D Di Marino, EJ Carr, N Oskolkov, V Lyssenko, D Franckaert, V Lagou, L Overbergh, J Vandenbuss, J Allemeersc, G Chabot-Roy, JE Dahlstrom, DR Laybutt, N Petrovsky, L Socha, K Gevaert, AM Jetten, D Lambrechts, MA Linterman, CC Goodnow, CJ Nolan, S Lesage, SM Schlenner, A Liston
Nat. Genet., 2016-03-21;48(5):519-27.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Pancreatic islet cell phenotype and endocrine function throughout diabetes development in non-obese diabetic mice.
Authors: Kornete, Mara, Beauchemin, Hugues, Polychronakos, Constant, Piccirillo, Ciriaco
Autoimmunity, 2013-02-04;46(4):259-68.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry