Home / Brachyury / Human/Mouse Brachyury PE-conjugated Antibody

Human/Mouse Brachyury PE-conjugated Antibody

Catalog #: IC2085P
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC2085P
Detection of Brachyury in D3 Mouse Cell Line by Flow Cytometry.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Brachyury PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Brachyury
Ser2-Glu202
Accession # O15178
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
D3 mouse embryonic stem cell line differentiated with mouse serum was fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) and NCI-H460 cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Brachyury antibody in D3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Brachyury in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was stained with Goat Anti-Human/Mouse Brachyury PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085P, filled histogram) or isotype control antibody (IC108P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry View Larger

Detection of Brachyury in NCI-H460 cells by Flow Cytometry. NCI-H460 cells were stained with Goat Anti-Human/Mouse Brachyury PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Brachyury

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Entrez Gene IDs
6862 (Human)
Alternate Names
brachyury protein; Brachyury; MGC104817; Protein T; SAVA; T brachyury (mouse) homolog; T brachyury homolog; T Brachyury Transcription Factor; T, brachyury homolog (mouse); T-Box Transcription Factor T; TBXT; TF; TFT

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Citations for Human/Mouse Brachyury PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesis
    Authors: Bisson, JA;Gordillo, M;Kumar, R;de Silva, N;Yang, E;Banks, KM;Shi, ZD;Lee, K;Yang, D;Chung, WK;Huangfu, D;Evans, T;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Cryptotanshinone is a candidate therapeutic agent for interstitial lung disease associated with a BRICHOS-domain mutation of SFTPC
    Authors: Motoyasu Hosokawa, Ryuta Mikawa, Atsuko Hagiwara, Yukiko Okuno, Tomonari Awaya, Yuki Yamamoto et al.
    iScience
  3. Stage-specific regulation of DNA methylation by TET enzymes during human cardiac differentiation
    Authors: Y Lan, KM Banks, H Pan, N Verma, GR Dixon, T Zhou, B Ding, O Elemento, S Chen, D Huangfu, T Evans
    Cell Reports, 2021-12-07;37(10):110095.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, ICC, Western Blot
  4. STRAP Deficiency Impairs Mouse Embryonic Stem Cells Lineage Commitment Through CYP26A1-Mediated Retinoic Acid Homeostasis
    Authors: L Jin, C Chang, KM Pawlik, A Datta, LM Johnson, T Vu, JL Napoli, PK Datta
    Stem Cells, 2018-07-17;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Non integrative strategy decreases chromosome instability and improves endogenous pluripotency genes reactivation in porcine induced pluripotent-like stem cells
    Authors: Annabelle Congras
    Sci Rep, 2016-06-01;6(0):27059.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Mesendogen, a novel inhibitor of TRPM6, promotes mesoderm and definitive endoderm differentiation of human embryonic stem cells through alteration of magnesium homeostasis
    Authors: Yijie Geng, Bradley Feng
    Heliyon
  7. Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells.
    Authors: Vijayaragavan K, Szabo E, Bosse M, Ramos-Mejia V, Moon RT, Bhatia M
    Cell Stem Cell, 2009-03-06;4(3):248-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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