Human/Mouse Carbonic Anhydrase II/CA2 Antibody

Detection of Human and Mouse Carbonic Anhydrase II/CA2 by Western Blot. Western blot shows lysates of human kidney tissue, Caki-2 human clear cell carcinoma epithelial cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse Carbonic Anhydrase II/ CA2 Monoclonal Antibody (Catalog # MAB2184) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Carbonic Anhydrase II/CA2 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Carbonic Anhydrase II/CA2 in Human Descending Colon and Ileum. Carbonic Anhydrase II/CA2 was detected in immersion fixed paraffin-embedded sections of human descending colon (left panel) and ileum (right panel) using Rat Anti-Human/Mouse Carbonic Anhydrase II/CA2 Monoclonal Antibody (Catalog # MAB2184) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue).

Detection of Mouse Carbonic Anhydrase II/CA2 by Western Blot Inhibition of induction of carbonic anhydrase II (CAII), anion exchange protein 2 (Ae2), chloride channel 7 (ClC-7), vacuolar-type H(+)-ATPase (V-ATPase), and cathapsin K (A,B), matrix metalloproteinase 9 (MMP-9) secretion (C), and bone resorption (D) by aesculetin. Raw 264.7 cells were cultured in minimum essential medium alpha medium ( alpha -MEM) with 50 ng/mL receptor activator of nuclear factor-kappa B ligand (RANKL) in the absence or presence of 1–10 μM aesculetin for 5 days. Whole-cell lysates were subject to SDS-PAGE and Western blot with a specific antibody against CAII, Ae2, ClC-7 V-ATPase, and cathapsin K (A,B). An equal volume of culture medium was subject to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot with a specific antibody against MMP-9 (C). beta -Actin was used as internal control. The bar graphs (mean ± SEM, n = 3) represent quantitative results of blots obtained from a densitometer. Respective values in double-bar graphs not sharing a small letter are significantly different at p < 0.05. The osteoclast bone resorption was assayed using a commercially available bone resorption assay kit ((D), three separate experiments). Attached cells were removed, and resorption pits on the plate were visualized under light microscopy. Scale bar = 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33203061), licensed under a CC-BY license. Not internally tested by R&D Systems.