Home / Carbonic Anhydrase II/CA2 / Human/Mouse Carbonic Anhydrase II/CA2 Antibody

Human/Mouse Carbonic Anhydrase II/CA2 Antibody

Catalog #: MAB2184
4 Citations Datasheet / COA / SDS
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MAB2184
MAB2184-SP
Detection of Human and Mouse Carbonic Anhydrase II/CA2 by Western Blot.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Carbonic Anhydrase II/CA2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Carbonic Anhydrase II/CA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CA-1, -3, -4, -5A, -5B, -6, -7, -8, or -9 is observed.
Source
Monoclonal Rat IgG2A Clone # 322706
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Carbonic Anhydrase II/CA2
Ser2-Lys260
Accession # P00918
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
0.5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Carbonic Anhydrase II/CA2 antibody by Western Blot. View Larger

Detection of Human and Mouse Carbonic Anhydrase II/CA2 by Western Blot. Western blot shows lysates of human kidney tissue, Caki-2 human clear cell carcinoma epithelial cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse Carbonic Anhydrase II/ CA2 Monoclonal Antibody (Catalog # MAB2184) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Carbonic Anhydrase II/CA2 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Carbonic Anhydrase II/CA2 antibody in Human Descending Colon and Ileum by Immunohistochemistry (IHC-P). View Larger

Carbonic Anhydrase II/CA2 in Human Descending Colon and Ileum. Carbonic Anhydrase II/CA2 was detected in immersion fixed paraffin-embedded sections of human descending colon (left panel) and ileum (right panel) using Rat Anti-Human/Mouse Carbonic Anhydrase II/CA2 Monoclonal Antibody (Catalog # MAB2184) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase II/CA2

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in the CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in the clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification (2).

References
  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Shah, G.N. et al. (2004) Hum. Mutat. 24:272.
Entrez Gene IDs
760 (Human)
Alternate Names
CA2; CAII; CA-IIEC 4.2.1.1; Car2; Carbonate dehydratase II; carbonic anhydrase 2; carbonic anhydrase B; Carbonic anhydrase C; Carbonic Anhydrase II; carbonic anhydrase IICAC; carbonic dehydratase

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Citations for Human/Mouse Carbonic Anhydrase II/CA2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Transcriptomic atlas and interaction networks of brain cells in mouse CNS demyelination and remyelination
    Authors: J Hou, Y Zhou, Z Cai, M Terekhova, A Swain, PS Andhey, RM Guimaraes, A Ulezko Ant, T Qiu, S Sviben, G Strout, JAJ Fitzpatric, Y Chen, S Gilfillan, DH Kim, SJ Van Dyken, MN Artyomov, M Colonna
    Cell Reports, 2023-03-21;42(4):112293.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Aesculetin Inhibits Osteoclastic Bone Resorption through Blocking Ruffled Border Formation and Lysosomal Trafficking
    Authors: W Na, EJ Lee, MK Kang, YH Kim, DY Kim, H Oh, SI Kim, SY Oh, YH Kang
    Int J Mol Sci, 2020-11-13;21(22):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Coumarin Ameliorates Impaired Bone Turnover by Inhibiting the Formation of Advanced Glycation End Products in Diabetic Osteoblasts and Osteoclasts
    Authors: EJ Lee, MK Kang, YH Kim, DY Kim, H Oh, SI Kim, SY Oh, W Na, YH Kang
    Biomolecules, 2020-07-15;10(7):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  4. Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and TREM2-independent cellular responses in Alzheimer's disease
    Authors: Y Zhou, WM Song, PS Andhey, A Swain, T Levy, KR Miller, PL Poliani, M Cominelli, S Grover, S Gilfillan, M Cella, TK Ulland, K Zaitsev, A Miyashita, T Ikeuchi, M Sainouchi, A Kakita, DA Bennett, JA Schneider, MR Nichols, SA Beausoleil, JD Ulrich, DM Holtzman, MN Artyomov, M Colonna
    Nat. Med., 2020-01-13;26(1):131-142.
    Species: Mouse
    Sample Types: Tissue
    Applications: IHC

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