Human/Mouse Caspase-7 Antibody Summary
Accession # P55210
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Caspase‑7 Precursor and Large Subunit by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line untreated (-) or treated (+) with 1 µM staurosporine (STS) for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Caspase-7 Monoclonal Antibody (Catalog # MAB823), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Caspase-7 precursor and large subunit at approximately 38 and 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
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Western Blot Shows Human Caspase‑7 Specificity by Using Knockout Cell Line. Western blot shows lysates of A549 human lung carcinoma parental cell line and Caspase-7 knockout A549 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse Caspase-7 Monoclonal Antibody (Catalog # MAB823) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Caspase-7 at approximately 32 kDa (as indicated) in the parental A549 cell line, but is not detectable in knockout A549 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-7
Caspase-7 (Cysteine-aspartic acid protease 7/Casp7; also CMH-1, ICE-LAP3 and Mch3) is a 32 kDa member of the peptidase C14A/IL-1 beta -converting family of enzymes (1, 2, 3). It is widely expressed, except in brain, and is best known as an integral component of the apoptotic cascade. Caspase-7 is considered to be an executioner caspase, as a downstream mediator of apoptotic-associated proteolysis (2, 3). Upon activation, Caspase-7 is known to utilize a Cys residue to cleave multiple substrates, including PARP, procaspase 6, Gas2 and calpstatin (1). Human procaspase-7 is a 34 - 36 kDa, 303 amino acid (aa) protein (4, 5, 6). Normally, it is an inactive homodimer (1, 2, 7, 8). But following an upstream signal that activates processing proteases, procaspase-7 undergoes proteolytic cleavage to generate an N-terminal 23 aa propeptide, a 175 aa p20/20 kDa subunit (aa 24 - 198), and a 105 aa C-terminal p12/12 kDa subunit (5). The p20 and p12 subunits noncovalently heterodimerize, and subsequently associate with another p20/p12 heterodimer to form an active antiparallel homodimer. Additional processing of p20 may remove aa 24 - 36 to generate p18, while additional processing of p12 will remove aa 199 - 206 to generate p11 (9, 10). Multiple proteases can use Caspase-7 as a substrate, and include caspase-1, -3, -8, and -10, granzyme B, calpain-1 and Caspase-7 itself (3, 6, 9, 11). Caspase-7 is found in both cytosol and nucleus, and possesses a potential KKKK nuclear localization signal between aa 38 - 41 that likely undergoes sumoylation (9, 12). There are two potential isoform variants, one which shows an alternate start site 33 aa upstream of the standard start site, and a second that shows a 105 aa substitution for aa 149 - 303. Human and mouse Caspase-7 are 82% aa identical at the amino acid level.
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Product Datasheets
Citations for Human/Mouse Caspase-7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Antitumour Activity of the Ribonuclease Binase from Bacillus pumilus in the RLS40 Tumour Model Is Associated with the Reorganisation of the miRNA Network and Reversion of Cancer-Related Cascades to Normal Functioning
Authors: ISE Mohamed, AV Sen'kova, AI Nadyrova, IA Savin, AV Markov, VA Mitkevich, AA Makarov, ON Ilinskaya, NL Mironova, MA Zenkova
Biomolecules, 2020-11-02;10(11):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
LQB?118 compound inhibits migration and induces cell death in glioblastoma cells
Authors: PS Bernardo, GHC Guimarães, FCC De Faria, GMDC Longo, GPF Lopes, CD Netto, PRR Costa, RC Maia
Oncol. Rep., 2019-11-06;43(1):346-357.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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