Human/Mouse CCR6 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB590G-025
FAB590G-100
Detection of CCR6 in Human Blood Lymphocytes by Flow Cytometry.
2 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse CCR6 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse CCR6-transfected rat RBL cells but not the parent cell line or cells transfected with mouse CCR5. Detects human CCR6 in flow cytometry.
Source
Monoclonal Rat IgG2A Clone # 140706
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
RBL-1 rat basophilic leukemia cell line transfected with mouse CCR6
Met1-Met367
Accession # O54689
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Titration recommended for optimal concentration with starting range of 0.1-1 µg/1 million cells. Samples used for this experiment were Human peripheral blood lymphocytes and B6 splenocytes 
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR6 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CCR6 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (FAB4867A) and either (A) Rat Anti-Human/Mouse CCR6 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB590G) or (B) Rat IgG2AAlexa Fluor 488 Isotype Control (IC006G). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of CCR6 in B6 splenocytes by Flow Cytometry. B6 splenocytes were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Human/Mouse CCR6 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB590G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Entrez Gene IDs
1235 (Human); 12458 (Mouse); 308163 (Rat)
Alternate Names
BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKRL3; CKR-L3; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPRCY4; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22

Product Datasheets

You must select a language.

x

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human/Mouse CCR6 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Transgenic Overexpression of Galectin-3 in Pancreatic beta Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33
    Authors: N Jovicic, I Petrovic, N Pejnovic, B Ljujic, M Miletic Ko, S Pavlovic, I Jeftic, A Djukic, I Srejovic, V Jakovljevi, ML Lukic
    Frontiers in Pharmacology, 2021-11-05;12(0):714683.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Expression and role of VLA-1 in resident memory CD8 T cell responses to respiratory mucosal viral-vectored immunization against tuberculosis
    Authors: S Haddadi, N Thanthrige, S Afkhami, A Khera, M Jeyanathan, Z Xing
    Sci Rep, 2017-08-25;7(1):9525.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.
    Authors: Funakoshi, Shuichi, Shimizu, Takeyuki, Numata, Osamu, Ato, Manabu, Melchers, Fritz, Ohnishi, Kazuo
    PLoS ONE, 2015-01-22;10(1):e0117566.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse CCR6 Alexa Fluor® 488-conjugated Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse CCR6 Alexa Fluor® 488-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse CCR6 Alexa Fluor® 488-conjugated Antibody
By Anonymous on 08/18/2017
Application: Flow Sample Tested: Mouse splenocytes Species: Mouse