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Human/Mouse/Chicken RGM-A Antibody

Catalog #: MAB2458
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2458
MAB2458-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Chicken RGM-A Antibody Summary

Species Reactivity
Human, Mouse, Chicken
Specificity
Detects mouse RGM-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant human RGM-A and recombinant chicken RGM is observed. In Western blots, approximately 25% cross-reactivity with recombinant mouse RGM-C is observed.
Source
Monoclonal Rat IgG2A Clone # 275407
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse RGM-A
Cys48-Gly421
Accession # Q6PCX7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Repulsive Guidance Molecule A/RGM-A (Catalog # 2459-RM)
Recombinant Mouse Repulsive Guidance Molecule A/RGM-A (Catalog # 2458-RG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RGM-A

RGM-A is the founding member of the RGM family of glycosylphosphatidylinositol (GPI)- anchored axon guidance proteins. It is a disulfide-linked dimer of the N‑terminal and C-terminal RGD-containing segments. RGM-A binds with high affinity to Neogenin.

Long Name
Repulsive Guidance Molecule A
Entrez Gene IDs
56963 (Human); 244058 (Mouse)
Alternate Names
RGM domain family member A; RGM domain family, member A; RGMA; RGM-A; RGMrepulsive guidance molecule A

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Citations for Human/Mouse/Chicken RGM-A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Inhibition of repulsive guidance molecule-a protects dopaminergic neurons in a mouse model of Parkinson's disease
    Authors: W Oda, Y Fujita, K Baba, H Mochizuki, H Niwa, T Yamashita
    Cell Death & Disease, 2021-02-15;12(2):181.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Repulsive guidance molecule-A (RGM-A) inhibits leukocyte migration and mitigates inflammation.
    Authors: Mirakaj V, Brown S, Laucher S, Steinl C, Klein G, Kohler D, Skutella T, Meisel C, Brommer B, Rosenberger P, Schwab JM
    Proc. Natl. Acad. Sci. U.S.A., 2011-04-05;108(16):6555-60.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-Fr

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