Human/Mouse Cited-2 Antibody Summary
Met1-Cys269
Accession # O35740
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Cited‑2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Cited-2 Monoclonal Antibody (Catalog # MAB5005) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cited-2 at approximately 30-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Cited‑2 in NIH‑3T3 Mouse Cell Line. Cited-2 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Rat Anti-Human/Mouse Cited-2 Monoclonal Antibody (Catalog # MAB5005) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cited-2
Cited-2 is a 270 amino acid protein that functions by binding to the CH1 region of CREB binding protein (CBP)/p300. This binding interferes with certain transcription factors like HIF1 alpha and STAT2 blocking their ability to bind and interact with CBP/p300. Cited-2 knockout mice die due to defects in heart and neural tube development. Mutations in Cited-2 have been reported in patients with congenital heart defects. It appears Cited-2 modulates apoptosis by a p53 independent pathway whereby the details still need to be elucidated.
Product Datasheets
Citations for Human/Mouse Cited-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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CDK7 Inhibition Is Effective in all the Subtypes of Breast Cancer: Determinants of Response and Synergy with EGFR Inhibition
Authors: Martina S. J. McDermott, Amanda C. Sharko, Jessica Munie, Susannah Kassler, Theresa Melendez, Chang-uk Lim et al.
Cells
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Exogenous WNT5A and WNT11 proteins rescue CITED2 dysfunction in mouse embryonic stem cells and zebrafish morphants
Authors: João M. A. Santos, Leonardo Mendes-Silva, Vanessa Afonso, Gil Martins, Rui S. R. Machado, João A. Lopes et al.
Cell Death & Disease
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TNFSF14/LIGHT, a Non-Canonical NF-kappa B Stimulus, Induces the HIF Pathway
Authors: Laura D’Ignazio, Michael Batie, Sonia Rocha
Cells
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Insulin Downregulates the Transcriptional Coregulator CITED2, an Inhibitor of Proangiogenic Function in Endothelial Cells
Authors: Xuanchun Wang, Samuel M. Lockhart, Thomas Rathjen, Hassan Albadawi, Ditte Sørensen, Brian T. O'Neill et al.
Diabetes
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