Human/Mouse COX4 Antibody Summary
no cross-reactivity with rhCOX-1 or rhCOX-2 is observed.
Asn39-Lys171
Accession # Q96KJ9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse COX4 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human COX4 Monoclonal Antibody (Catalog # MAB6980) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For addtional reference, recombinant human COX4-I1 (5 ng/lane) and recombinant human COX4-I2 (2.5 ng/lane) were included. A specific band was detected for COX4 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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COX4 in NIH‑3T3 Mouse Cell Line. COX4 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human/Mouse COX4 Monoclonal Antibody (Catalog # MAB6980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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COX4 in HeLa Human Cell Line. COX4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse COX4 Monoclonal Antibody (Catalog # MAB6980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: COX4
COX4-I2 (Cytochrome c oxidase subunit 4) is a 21-22 kDa member of the Cytochrome c oxidase IV family of proteins and is predominantly expressed in lung. It is a component of COX, an inner mitochondrial membrane multimeric dimer that catalyzes the transfer of electrons from Cytochrome c to dioxygen. COX4-I2 is induced following blockade of mitochondrial respiration, leading to increased production of reactive oxygen species and necrosis. The ubiquitously expressed COX4-I1 is the product of a different gene and shares only 50% aa identity with COX4-I2. Within aa 39-171 (the oxidase domain), human COX4-I2 shares 84% and 81% aa sequence identity with mouse and rat COX4-I2, respectively.
Product Datasheets
Citations for Human/Mouse COX4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Mitochondrial PARP1 regulates NAD+-dependent poly ADP-ribosylation of mitochondrial nucleoids
Authors: JH Lee, M Hussain, EW Kim, SJ Cheng, AKL Leung, NB Fakouri, DL Croteau, VA Bohr
Experimental & Molecular Medicine, 2022-12-06;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Lipid storage droplet protein 5 reduces sodium palmitate?induced lipotoxicity in human normal liver cells by regulating lipid metabolism?related factors
Authors: X Ma, F Cheng, K Yuan, K Jiang, T Zhu
Mol Med Rep, 2019-06-06;20(2):879-886.
Species: Human
Sample Types: Cell Lyates
Applications: Western Blot -
SIRT3 diminishes inflammation and mitigates endotoxin-induced acute lung injury
Authors: D Kurundkar, AR Kurundkar, NB Bone, EJ Becker, W Liu, B Chacko, V Darley-Usm, JW Zmijewski, VJ Thannickal
JCI Insight, 2019-01-10;4(1):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Frataxin-deficient neurons and mice models of Friedreich ataxia are improved by TAT-MTScs-FXN treatment
Authors: E Britti, F Delaspre, A Feldman, M Osborne, H Greif, J Tamarit, J Ros
J. Cell. Mol. Med., 2017-10-05;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
APOL1 risk variants in individuals of African genetic ancestry drive endothelial cell defects that exacerbate sepsis
Authors: Junnan Wu, Ziyuan Ma, Archana Raman, Pazit Beckerman, Poonam Dhillon, Dhanunjay Mukhi et al.
Immunity
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