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Human/Mouse CX3CR1 Antibody

Catalog #: AF5825
16 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5825
AF5825-SP
Detection of Human and Mouse CX3CR1 by Western Blot.
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Product Details
Citations (16)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse CX3CR1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse CX3CR1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CX3CR1
Met1-Thr32, Leu92-Lys104, Thr169-Val196, Lys258-Leu274
Accession # Q9Z0D9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse CX3CR1 antibody by Western Blot. View Larger

Detection of Human and Mouse CX3CR1 by Western Blot. Western blot shows lysates of M1 mouse myeloid leukemia cell line and NK-92 human natural killer lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5825) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CX3CR1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of CX3CR1 antibody in M1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of CX3CR1 in M1 Mouse Cell Line by Flow Cytometry. M1 mouse myeloid leukemia cell line was stained with Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5825, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CX3CR1

CX3CR1 (CX3C chemokine receptor 1; also fractalkine receptor, GPCR13 and V28) is a 40 kDa (predicted) member of the δ-group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1lo monocytes plus smooth muscle and mast cells. CX3CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354 amino acids (aa) in length. It contains a 32 aa N-terminal extracellular region that shows no glycosylation, and a 56 aa C-terminal cytoplasmic domain. Over aa 1‑32, 91‑104, 169‑196 and 258‑274 collectively, mouse CX3CR1 shares 96% and 77% aa identity with rat and human CX3CR1, respectively.

Long Name
CX3C Chemokine Receptor 1
Entrez Gene IDs
1524 (Human); 13051 (Mouse); 171056 (Rat)
Alternate Names
Beta chemokine receptor-like 1; CCRL1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMKbRL1; CMK-BRL1; CMK-BRL-1; CMKDR1; CX3C chemokine receptor 1; CX3CR1; Fractalkine receptor; G protein-coupled receptor 13; GPR13G-protein coupled receptor 13; GPRV28; V28; V28C-X3-C CKR-1

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Citations for Human/Mouse CX3CR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Distinct functions of chemokine receptor axes in the atherogenic mobilization and recruitment of classical monocytes.
    Authors: Soehnlein Oliver, Drechsler Maik, Doring Yvonne et al.
    EMBO Mol Med.
  2. Human iPSC-derived myelinating organoids and globoid cells to study Krabbe Disease
    Authors: Evans, LMP;Gawron, J;Sim, FJ;Laura Feltri, M;Marziali, LN;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Organoid
    Applications: Immunohistochemistry, Immunocytochemistry
  3. CX3CL1 induces cell migration and invasion through ICAM‐1 expression in oral squamous cell carcinoma cells
    Authors: Chia‐Yu Wu, Pei‐Wen Peng, Ting‐Yi Renn, Chia‐Jung Lee, Tsung‐Ming Chang, Augusta I‐Chin Wei et al.
    Journal of Cellular and Molecular Medicine
  4. Glabridin Therapy Reduces Chronic Allodynia, Spinal Microgliosis, and Dendritic Spine Generation by Inhibiting Fractalkine-CX3CR1 Signaling in a Mouse Model of Tibial Fractures
    Authors: Long, J;Liu, H;Qiu, Z;Xiao, Z;Lu, Z;
    Brain sciences
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Crosstalk between cytotoxic CD8+ T cells and stressed cardiomyocytes triggers development of interstitial cardiac fibrosis in hypertensive mouse hearts
    Authors: Kurt Brassington, Peter Kanellakis, Anh Cao, Ban-Hock Toh, Karlheinz Peter, Alex Bobik et al.
    Frontiers in Immunology
  6. Dynamic changes of CX3CL1/CX3CR1 axis during microglial activation and motor neuron loss in the spinal cord of ALS mouse model
    Authors: Jingjing Zhang, Yufei Liu, Xinyao Liu, Song Li, Cheng Cheng, Sheng Chen et al.
    Translational Neurodegeneration
  7. Novel adherent CD11b+Gr-1+tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment
    Authors: T Tsubaki, T Kadonosono, S Sakurai, T Shiozawa, T Goto, S Sakai, T Kuchimaru, T Sakamoto, H Watanabe, G Kondoh, S Kizaka-Kon
    Oncotarget, 2018-01-29;9(13):11209-11226.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Migration-based selections of antibodies that convert bone marrow into trafficking microglia-like cells that reduce brain amyloid ?
    Authors: KH Han, BM Arlian, MS Macauley, JC Paulson, RA Lerner
    Proc. Natl. Acad. Sci. U.S.A., 2018-01-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Hepatectomy leads to loss of TRAIL-expressing liver NK cells via downregulation of the CXCL9-CXCR3 axis in mice
    Authors: T Yano, M Ohira, R Nakano, Y Tanaka, H Ohdan
    PLoS ONE, 2017-10-31;12(10):e0186997.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. A CCR2+ myeloid cell niche required for pancreatic ? cell growth
    Authors: K Mussar, S Pardike, TM Hohl, G Hardiman, V Cirulli, L Crisa
    JCI Insight, 2017-08-03;2(15):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. MicroRNA-101a regulates microglial morphology and inflammation
    Authors: R Saika, H Sakuma, D Noto, S Yamaguchi, T Yamamura, S Miyake
    J Neuroinflammation, 2017-05-30;14(1):109.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  12. Selective suppression of the ? isoform of p38 MAPK rescues late-stage tau pathology
    Alzheimers Res Ther, 2016-12-15;8(1):54.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. MKEY, a Peptide Inhibitor of CXCL4-CCL5 Heterodimer Formation, Protects Against Stroke in Mice
    J Am Heart Assoc, 2016-09-15;5(9):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
    Authors: Kowalewska P, Patrick A, Fox-Robichaud A
    PLoS ONE, 2014-09-03;9(9):e104537.
    Species: Mouse
    Sample Types: In Vivo
    Applications: IHC-Fr
  15. CX3CL1(+) Microparticles Mediate the Chemoattraction of Alveolar Macrophages toward Apoptotic Acute Promyelocytic Leukemic Cells
    Authors: Wen-Hui Tsai, Chung-Hung Shih, Shan-Yu Feng, I-Ting Li, Shao-Chi Chang, Yu-Chieh Lin et al.
    Cellular Physiology and Biochemistry
  16. Fractalkine is a novel human adipochemokine associated with type 2 diabetes.
    Authors: Shah R, Hinkle CC, Ferguson JF, Mehta NN, Li M, Qu L, Lu Y, Putt ME, Ahima RS, Reilly MP
    Diabetes, 2011-05-01;60(5):1512-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human/Mouse CX3CR1 Antibody
By Anonymous on 09/11/2019
Application: WB Sample Tested: HMC3 (human microglia cell line) lysate Species: Human
Western Blot Human/Mouse CX3CR1 Antibody AF5825