Human/Mouse DACT3/Dapper 3 Antibody

Catalog #: AF5767 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5767
AF5767-SP

Detection of Human and Mouse DACT3/Dapper 3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse DACT3/Dapper 3 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse DACT3/Dapper 3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse DACT2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse DACT3/Dapper 3
Pro486-Lys587
Accession # Q0PHV7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse DACT3/Dapper 3 by Western Blot. Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse DACT3/Dapper 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for DACT3/Dapper 3 at approximately 45 kDa with human brain and 50 kDa with mouse brain (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DACT3/Dapper 3

Dapper 3 (also Dact 3) is a 63 kDa (predicted) member of the DACT (Dapper/Frodo) family of Dvl-binding proteins. It is expressed in cerebral cortex and uterus where it antagonizes Wnt/ beta -catenin signaling. When present in colorectal tumor cells, it initiates apoptosis. Both effects are likely the result of a Dapper 3 interaction with Dvl2, thereby blocking beta -catenin entry into the nucleus. Mouse Dapper 3 is 610 amino acids (aa) in length It contains one coiled-coil region (aa 63-87), an Arg-rich domain (aa 351-476), and a PZD-binding motif (aa 607-610). There is one potential splice variant that shows a 45 aa substitution for aa 413-610. In human Dact 3 the frameshift at position 119 is reported to yield a protein product with estimated moleqular weight of 41 kDa. Over aa 486-587, mouse dapper 3 shares 82% and 99% aa identity with human and rat dapper 3, respectively.

Long Name

Dapper, Antagonist of beta-Catenin, Homolog 3

Entrez Gene IDs

147906 (Human); 629378 (Mouse); 499088 (Rat)

Alternate Names

Antagonist of beta-catenin Dapper homolog 3; arginine rich region 1; Arginine-rich region 1 protein; DACT3; Dapper 3; dapper homolog 3; dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis); MGC15476; RRR1