Human/Mouse DAPP1 Antibody Summary
Gly2-Ser163
Accession # Q9UN19
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human and Mouse DAPP1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DAPP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7024) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DAPP1 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DAPP1
DAPP1 (Dual Adaptor for Phosphotyrosine and 3-Phosphoinosidides 1; also Bam32 and PHISH) is a 31-32 kDa member of Ig-superfamily of proteins. It shows restricted expression, being found in mast cells, dendritic cells, and germinal center B cells. With respect to B cells, and upon B cell receptor engagement, PI3-kinase is activated, resulting in the generation of membrane-embedded PI(3,4)P2. This product serves as a ligand for cytosolic DAPP1, resulting in its immobilization at the cell membrane. Here, it is phosphorylated on Tyr139, directly regulating HPK1 (hematopoietic progenitor kinase 1) activity, and indirectly regulation HPK1 downstream targets ERK and JNK. Functionally, DAPP1 plays a role in BCR internalization, antibody isotype switching, antigen processing and presentation, and B cell survival. Human DAPP1 is 280 amino acids (aa) in length. It contains one SH2 domain (aa 35‑129), a utilized phosphorylation site at Tyr139 and a C-terminal PH domain (aa 164‑259). When phosphorylated, the observed MW of DAPP1 may be increased by 2-4 kDa in SDS-PAGE. There are four potential alternative splice variants. Two contain a five and 22 aa substitution for aa 259-280, respectively, while a third possesses a 14 aa substitution for aa 1-229, and a fourth shows deletions of aa 35‑75 and aa 180-200 coupled to a three aa substitution for aa 249-280. Over aa 1-163, human DAPP1 shares 91% aa sequence identity with mouse DAPP1.
Product Datasheets
Citation for Human/Mouse DAPP1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Identification of PtdIns(3,4)P2 effectors in human platelets using quantitative proteomics
Authors: Tom N. Durrant, Samantha F. Moore, Asha L. Bayliss, Yiliang Jiang, Elizabeth W. Aitken, Marieangela C. Wilson et al.
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse DAPP1 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse DAPP1 Antibody and earn rewards!
Have you used Human/Mouse DAPP1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
