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Human/Mouse DNMT3A Antibody

Catalog #: MAB63151
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB63151
MAB63151-SP
Detection of Human and Mouse DNMT3A by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse DNMT3A Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human DNMT3A in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human DNMT1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 681615
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human DNMT3A
Ala353-Lys486
Accession # Q9Y6K1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse DNMT3A antibody by Western Blot. View Larger

Detection of Human and Mouse DNMT3A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, U2OS human osteosarcoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human DNMT3A Monoclonal Antibody (Catalog # MAB63151) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DNMT3A at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DNMT3A

DNMT3A (DNA [cytosine-5]-methyltransferase 3A) is a 130 kDa member of the C5-methlytransferase family of molecules.  It is expressed both during development and in virtually all adult tissues except for small intestine.  DNMT3A is a nuclear protein that is found associated with heterochromatin.  It possesses DNA methyltransferase activity, and acts in a complex with DNMT3L in embryonic stem cells, forming a (3A)2:(3L)2 heterotetramer.  It also interacts with SETDB1 to form a transcriptional repressor.  Human DNMT3A is 912 amino acids (aa) in length.  It contains one ProTrpTrpPro domain (aa 292-350) and one ADD-type zinc finger region (aa 494-586).  There is one 100 kDa splice variant termed DNMT3a2 that contains an alternative start site at Met224, and preferentially interacts with euchromatin.  Other potential splice variants show a 16 aa substitution for aa 151-912, and a 24 aa substitution for aa 1-213.  Over aa 353-486, human DNMT3A shares 96% aa identity with mouse DNMT3A.  

Long Name
DNA [Cytosine-5-]-Methyltransferase 3A
Entrez Gene IDs
1788 (Human); 13435 (Mouse); 444984 (Rat)
Alternate Names
DNA (cytosine-5-)-methyltransferase 3 alpha; DNA (cytosine-5)-methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA Methyltransferase 3 Alpha; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; DNMT3A; DNMT3A2; EC 2.1.1.37; M.HsaIIIA; TBRS

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Citations for Human/Mouse DNMT3A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. TET2 and DNMT3A Mutations Exert Divergent Effects on DNA Repair and Sensitivity of Leukemia Cells to PARP Inhibitors
    Authors: Silvia Maifrede, Bac Viet Le, Margaret Nieborowska-Skorska, Konstantin Golovine, Katherine Sullivan-Reed, Wangisa M.B. Dunuwille et al.
    Cancer Research
  2. Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation
    Authors: Jiajun Zhu, Mirela Berisa, Simon Schwörer, Weige Qin, Justin R. Cross, Craig B. Thompson
    Cell Metabolism

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