Home / EphB2 / Human/Mouse EphB2 Antibody

Human/Mouse EphB2 Antibody

Catalog #: AF467
62 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF467
AF467-SP
Detection of Recombinant Human and Mouse EphB2 by Western Blot.
11 Images
Product Details
Citations (62)
FAQs
Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse EphB2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse and human EphB2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant rat (rr) EphB1, recombinant mouse (rm) EphA8, rmEphA6, rmEphB6, rmEphA3, rmEphA4, rmEphA7, rrEphA5, recombinant human EphA1, rmEphA2 and rmEphB3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphB2
Val27-Lys548
Accession # P54763
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
20 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
1-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human and Mouse EphB2 antibody by Western Blot. View Larger

Detection of Recombinant Human and Mouse EphB2 by Western Blot. Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EphB2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Flow Cytometry Detection of EphB2 antibody in COLO 205 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of EphB2 in COLO 205 Human Cell Line by Flow Cytometry. COLO 205 human colorectal adenocarcinoma cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of EphB2 antibody in D3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of EphB2 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry EphB2 antibody in MBA-MB-468 Human Cell Line by Immunocytochemistry (ICC). View Larger

EphB2 in MBA‑MB‑468 Human Cell Line. EphB2 was detected in immersion fixed MBA-MB-468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry EphB2 antibody in Embryonic Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

EphB2 in Embryonic Mouse Brain. EphB2 was detected in immersion fixed frozen sections of embryonic mouse brain (15 d.p.c.) using 15 µg/mL Goat Anti-Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry EphB2 antibody in Human Esophageal Squamous Cell Carcinoma by Immunohistochemistry (IHC-P). View Larger

EphB2 in Human Esophageal Squamous Cell Carcinoma. EphB2 was detected in immersion fixed paraffin-embedded sections of human esophageal squamous cell carcinoma using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry EphB2 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

EphB2 in Mouse Embryo. EphB2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Human EphB2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human EphB2 by Simple WesternTM. Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA-MB-468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at approximately 139 and 146 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry/ Immunofluorescence Detection of Human EphB2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human EphB2 by Immunocytochemistry/Immunofluorescence Colorectal tumours maintain tissue organisation similar to normal colon.Detection of EPHB2 (green) and ERBB3 (red, A and B) by co-immunofluorescence in normal colon (A) and colorectal cancer (B) (DAPI, blue). Scale bar, 50μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26367378), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse EphB2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse EphB2 by Immunocytochemistry/Immunofluorescence Expression of EphB2 increases and is activated on HSCs after chronic CCl4 -induced liver injury. (a) Isolated cell fractions from livers of mice subjected to chronic CCl4 injections were analyzed for EphB2, Ephrin-B1, Ephrin-B2 and Ephrin-B3 mRNA levels using RT-qPCR. Results are shown as fold change compared to liver cell fractions obtained from vehicle-treated controls. Error bars represent mean ± SEM.; n = 6 animals; CD11b = macrophages, LSEC = Liver sinusoidal endothelial cells, HEP = Hepatocytes and HSCs = Hepatic stellate cells. (b) OCT liver sections from C57BL/6 J mice chronically injected with CCl4 or vehicle (oil) controls were stained with EphB2 (red), alpha SMA (green) and DAPI/DNA (blue) and analyzed using confocal microscopy. Scale bar = 100 µm, “C” denotes the central vein. All images are representative of 5 mice per group. (c) OCT liver sections from C57BL/6 J mice chronically injected with CCl4 or vehicle controls were stained with phospho-EphB1/EphB2-Y594 (red), PDGFR beta (green) and DAPI/DNA (blue) and analyzed using confocal microscopy. Scale bar = 50 µm. All images are representative of 5 mice per group. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29416088), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human EphB2 by Western Blot View Larger

Detection of Human EphB2 by Western Blot Increased cell-cell adhesion within one cell population is required for the formation of tightly packed cell clusters.A) Simulation of cell-cell segregation using the same adhesion term in both cell types (Aeph  =  Aephrin  = 100, left panel) vs. increased adhesion only in the green cell population (Aeph  = 110, Aephrin  = 100, right panel). Both simulations started with the same number of Eph (green) and ephrin (black) expressing cells. In the “Equal adhesion” case, an ‘Islands-in-a-sea’ pattern is less apparent. B) Representative images from segregation assays of unlabelled ephrin-B1 cells co-cultured with Cell Tracker-green labelled (green staining) EphB2 cells, without (left) or with E-cadherin-cherry expression (red staining, right); scale bar, 75 µm. C) Quantitation of cell densities in the cell clusters shown in B (n = 10). D) Western blot analysis of lysates from parental and E-cadherin-cherry-transduced cells, using the indicated antibodies. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0111803), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphB2

EphB2, also known as Cek5, Nuk, Erk, Qek2, Tyro5, Sek3, Hek5, and Drt (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and the cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB2 has been shown to bind ephrin-B1, ephrin-B2, and ephrin-B3 (2, 3). The extracellular domains of human and mouse EphB2 share 99% amino acid identity. Only membrane-bound or
Fc‑clustered ligands are capable of activating the receptor in vitro. Soluble monomeric ligands bind the receptor but do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
Long Name
Eph Receptor B2
Entrez Gene IDs
2048 (Human); 13844 (Mouse)
Alternate Names
CAPB; Cek5; Drt; DRTEphB2; EC 2.7.10; EC 2.7.10.1; EK5; elk-related tyrosine kinase; EPH receptor B2; eph tyrosine kinase 3; EphB2; EPH-like kinase 5; ephrin type-B receptor 2; EPHT3MGC87492; EPTH3; Erk; ERKHek5; Hek5; Nuk; PCBC; protein-tyrosine kinase HEK5; Qek2; Renal carcinoma antigen NY-REN-47; Sek3; Tyro5; Tyrosine-protein kinase receptor EPH-3; Tyrosine-protein kinase TYRO5

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human/Mouse EphB2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

62 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration.
    Authors: Clements MP, Byrne E, Camarillo Guerrero LF et al.
    Neuron.
  2. Key role for EphB2 receptor in kidney fibrosis
    Authors: Zhimin Huang, Simeng Liu, Anna Tang, Laith Al-Rabadi, Mark Henkemeyer, Patrice N. Mimche et al.
    Clinical Science
  3. Ephrin‐B2 prevents N‐methyl‐D‐aspartate receptor antibody effects on memory and neuroplasticity
    Authors: Jesús Planagumà, Holger Haselmann, Francesco Mannara, Mar Petit‐Pedrol, Benedikt Grünewald, Esther Aguilar et al.
    Annals of Neurology
  4. Eph-mediated tyrosine phosphorylation of citron kinase controls abscission
    J Cell Biol, 2016-08-22;214(5):555-69.
  5. Role of forward and reverse signaling in Eph receptor and ephrin mediated cell segregation
    Authors: Zhonglin Wu, Tim G. Ashlin, Qiling Xu, David G. Wilkinson
    Experimental Cell Research
  6. Exosomes mediate cell contact–independent ephrin-Eph signaling during axon guidance
    Authors: Jingyi Gong, Roman Körner, Louise Gaitanos, Rüdiger Klein
    Journal of Cell Biology
  7. Characterization of rectal, proximal and distal colon cancers based on clinicopathological, molecular and protein profiles
    Authors: P Minoo, I Zlobec, M Peterson, L Terracciano, A Lugli
    International Journal of Oncology
  8. Retrograde movements determine effective stem cell numbers in the intestine
    Authors: M Azkanaz, B Corominas-, SIJ Ellenbroek, L Bruens, AT Webb, D Laskaris, KC Oost, SJA Lafirenze, K Annusver, HA Messal, S Iqbal, DJ Flanagan, DJ Huels, F Rojas-Rodr, M Vizoso, M Kasper, OJ Sansom, HJ Snippert, P Liberali, BD Simons, P Katajisto, E Hannezo, J van Rheene
    Nature, 2022-07-13;0(0):.
  9. Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury
    Authors: Victor H. Guaiquil, Cissy Xiao, Daniel Lara, Greigory Dimailig, Qiang Zhou
    Experimental Eye Research
  10. Mule Regulates the Intestinal Stem Cell Niche via the Wnt Pathway and Targets EphB3 for Proteasomal and Lysosomal Degradation
    Authors: Relation T, Yi T, Guess AJ et al.
    Cell Stem Cell
  11. Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer
    Authors: C Morral, J Stanisavlj, X Hernando-M, E Mereu, A Álvarez-Va, C Cortina, D Stork, F Slebe, G Turon, G Whissell, M Sevillano, A Merlos-Suá, À Casanova-M, C Moutinho, SW Lowe, LE Dow, A Villanueva, E Sancho, H Heyn, E Batlle
    Cell Stem Cell, 2020-05-11;26(6):845-861.e12.
  12. Ephrin reverse signaling mediates palatal fusion and epithelial-to-mesenchymal transition independently of tgf beta 3
    Authors: Maria J. Serrano, Jingpeng Liu, Kathy K.H. Svoboda, Ali Nawshad, M. Douglas Benson
    Journal of Cellular Physiology
  13. NMDA receptors control development of somatosensory callosal axonal projections
    Authors: Jing Zhou, Yong Lin, Trung Huynh, Hirofumi Noguchi, Jeffrey O Bush, Samuel J Pleasure
    eLife
  14. Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function
    Authors: Chang, C;Banerjee, SL;Park, SS;Zhang, X;Cotnoir-White, D;Desbois, M;Grill, B;Kania, A;
    bioRxiv : the preprint server for biology
    Species: Human, Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. GluN3A subunit tunes NMDA receptor synaptic trafficking and content during postnatal brain development
    Authors: Gonz�lez-Gonz�lez, IM;Gray, JA;Ferreira, J;Conde-Dusman, MJ;Bouchet, D;Perez-Ota�o, I;Groc, L;
    Cell reports
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  16. Role of EphB2/ephrin-B1 signalling in the development and progression of obesity-associated colorectal cancer
    Authors: Y Suzuki, K Okabayashi, H Hasegawa, M Tsuruta, R Seishima, T Tokuda, Y Kitagawa
    Oncology Letters, 2022-07-19;24(3):316.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks
    Authors: SL Banerjee, F Lessard, FJM Chartier, K Jacquet, AI Osornio-He, V Teyssier, K Ghani, N Lavoie, JN Lavoie, M Caruso, P Laprise, S Elowe, JP Lambert, N Bisson
    Cell Reports, 2022-07-05;40(1):111031.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting
    Authors: AE Lewis, A Kuwahara, J Franzosi, JO Bush
    Cell Reports, 2022-03-15;38(11):110510.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  19. Accumulation of Paneth Cells in Early Colorectal Adenomas Is Associated with Beta-Catenin Signaling and Poor Patient Prognosis
    Authors: E López-Arri, B Yan, T Lobo-Jarne, Y Guillén, S Menéndez, M Andreu, A Bigas, M Iglesias, L Espinosa
    Cells, 2021-10-28;10(11):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. Tyrosine phosphorylation of DEPTOR functions as a molecular switch to activate mTOR signaling
    Authors: L M Gagné, N Morin, N Lavoie, N Bisson, JP Lambert, FA Mallette, MÉ Huot
    The Journal of Biological Chemistry, 2021-10-09;297(5):101291.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Identification of tetracycline combinations as EphB1 tyrosine kinase inhibitors for treatment of neuropathic pain
    Authors: MS Ahmed, P Wang, NUN Nguyen, Y Nakada, I Menendez-M, M Ismail, R Bachoo, M Henkemeyer, HA Sadek, ES Kandil
    Proceedings of the National Academy of Sciences of the United States of America, 2021-03-09;118(10):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  22. Ephrin-B2 paces neuronal production in the developing neocortex
    Authors: A Kischel, C Audouard, MA Fawal, A Davy
    BMC Dev. Biol., 2020-05-13;20(1):12.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  23. Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling
    Authors: ST Wei, YC Huang, ML Hsieh, YJ Lin, WC Shyu, HC Chen, CH Hsieh
    Cell Death Dis, 2020-05-04;11(5):307.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  24. EPH receptor B2 stimulates human monocyte adhesion and migration independently of its EphrinB ligands
    Authors: D Vreeken, CS Bruikman, SML Cox, H Zhang, R Lalai, A Koudijs, AJ van Zonnev, GK Hovingh, JM van Gils
    J. Leukoc. Biol., 2020-04-26;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  25. Generation of intestinal organoids derived from human pluripotent stem cells for drug testing
    Authors: S Yoshida, H Miwa, T Kawachi, S Kume, K Takahashi
    Sci Rep, 2020-04-06;10(1):5989.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Positive surface charge of GluN1 N-terminus mediates the direct interaction with EphB2 and NMDAR mobility
    Authors: HR Washburn, NL Xia, W Zhou, YT Mao, MB Dalva
    Nat Commun, 2020-01-29;11(1):570.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay, ICC
  27. CD74 signaling links inflammation to intestinal epithelial cell regeneration and promotes mucosal healing
    Authors: L Farr, S Ghosh, N Jiang, K Watanabe, M Parlak, R Bucala, S Moonah
    Cell Mol Gastroenterol Hepatol, 2020-01-28;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  28. Spatial EGFR Dynamics and Metastatic Phenotypes Modulated by Upregulated EphB2 and Src Pathways in Advanced Prostate Cancer
    Authors: YL Liu, AM Horning, B Lieberman, M Kim, CK Lin, CN Hung, CW Chou, CM Wang, CL Lin, NB Kirma, MA Liss, R Vasisht, EP Perillo, K Blocher, H Horng, JA Taverna, J Ruan, TE Yankeelov, AK Dunn, TH Huang, HC Yeh, CL Chen
    Cancers (Basel), 2019-12-01;11(12):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. The Schizophrenia Susceptibility Gene OPCML Regulates Spine Maturation and Cognitive Behaviors through Eph-Cofilin Signaling
    Authors: Z Zhang, M Ye, Q Li, Y You, H Yu, Y Ma, L Mei, X Sun, L Wang, W Yue, R Li, J Li, D Zhang
    Cell Rep, 2019-10-01;29(1):49-61.e7.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  30. EphB2-dependent signaling promotes neuronal excitotoxicity and inflammation in the acute phase of ischemic stroke
    Authors: AS Ernst, LI Böhler, AM Hagenston, A Hoffmann, S Heiland, C Sticht, M Bendszus, M Hecker, H Bading, HH Marti, T Korff, R Kunze
    Acta Neuropathol Commun, 2019-02-05;7(1):15.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Simple Western
  31. EphB2 receptor tyrosine kinase promotes hepatic fibrogenesis in mice via activation of hepatic stellate cells
    Authors: PN Mimche, CM Lee, SM Mimche, M Thapa, A Grakoui, M Henkemeyer, TJ Lamb
    Sci Rep, 2018-02-07;8(1):2532.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  32. Generation of Mouse and Human Organoid-Forming Intestinal Progenitor Cells by Direct Lineage Reprogramming
    Authors: S Miura, A Suzuki
    Cell Stem Cell, 2017-09-21;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  33. Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon
    Authors: YH Liu, JW Tsai, JL Chen, WS Yang, PC Chang, PL Cheng, DL Turner, Y Yanagawa, TW Wang, JY Yu
    Sci Rep, 2017-03-09;7(0):42895.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  34. Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
    Authors: N Tüysüz, L van Bloois, S van den Br, H Begthel, MM Verstegen, LJ Cruz, L Hui, LJ van der La, J de Jonge, R Vries, E Braakman, E Mastrobatt, JJ Cornelisse, H Clevers, D Ten Berge
    Nat Commun, 2017-03-06;8(0):14578.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  35. Blocking the Interaction between EphB2 and ADDLs by a Small Peptide Rescues Impaired Synaptic Plasticity and Memory Deficits in a Mouse Model of Alzheimer's Disease
    Authors: Can Gao
    J. Neurosci., 2016-11-23;36(47):11959-11973.
    Species: Rat
    Sample Types: Protein, Whole Cells
    Applications: ICC, Western Blot
  36. Induction of endoplasmic reticulum stress by deletion of Grp78 depletes Apc mutant intestinal epithelial stem cells
    Oncogene, 2016-11-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  37. Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation
    Authors: AK O'Neill, AA Kindberg, TK Niethamer, AR Larson, HH Ho, ME Greenberg, JO Bush
    J. Cell Biol., 2016-10-17;215(2):217-229.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  38. The transcription factor XBP1s restores hippocampal synaptic plasticity and memory by control of the Kalirin-7 pathway in Alzheimer model
    Mol Psychiatry, 2016-09-20;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  39. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance
    Nat Commun, 2016-07-29;7(0):12329.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  40. Tissue-Specific Effects of Reduced beta-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium.
    Authors: Feng Y, Sakamoto N, Wu R, Liu J, Wiese A, Green M, Green M, Akyol A, Roy B, Zhai Y, Cho K, Fearon E
    PLoS Genet, 2015-11-03;11(11):e1005638.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  41. GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model.
    Authors: Dong L, Gao F, Wang X, Miao Y, Wang S, Wu Y, Li F, Wu J, Cheng X, Sun X, Yang X, Wang Z
    J Neurosci, 2015-04-01;35(13):5409-21.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  42. A mathematical model for eph/ephrin-directed segregation of intermingled cells.
    Authors: Aharon, Rotem, Janes, Peter W, Burgess, Anthony, Hamza, Kais, Klebaner, Fima, Lackmann, Martin
    PLoS ONE, 2014-12-01;9(12):e111803.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  43. Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.
    Authors: Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perriere N, Guillevic O, Couraud P, Uzan G
    PLoS ONE, 2014-01-02;9(1):e84179.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  44. Reelin induces EphB activation.
    Authors: Bouche E, Romero-Ortega M, Henkemeyer M, Catchpole T, Leemhuis J, Frotscher M, May P, Herz J, Bock H
    Cell Res, 2013-01-15;23(4):473-90.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  45. Neuropsin cleaves EphB2 in the amygdala to control anxiety.
    Authors: Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R
    Nature, 2011-04-20;473(7347):372-5.
    Species: Mouse
    Sample Types: In Vivo, Tissue Homogenates, Whole Cells, Whole Tissue
    Applications: IHC-Fr, Immunoprecipitation, Neutralization, Western Blot
  46. Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression.
    Authors: Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y
    Biochem. Biophys. Res. Commun., 2011-01-26;405(4):521-6.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  47. Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism.
    Authors: Bush JO, Soriano P
    Genes Dev., 2009-06-10;23(13):1586-99.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Immunoprecipitation, Western Blot
  48. EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts.
    Authors: Allan EH, Hausler KD, Wei T, Gooi JH, Quinn JM, Crimeen-Irwin B, Pompolo S, Sims NA, Gillespie MT, Onyia JE, Martin TJ
    J. Bone Miner. Res., 2008-08-01;23(8):1170-81.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  49. Ephrin-B reverse signaling promotes structural and functional synaptic maturation in vivo.
    Authors: Lim BK, Matsuda N, Poo MM
    Nat. Neurosci., 2008-01-13;11(2):160-9.
    Species: Xenopus
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  50. Phosphorylation of ephrin-B1 regulates dissemination of gastric scirrhous carcinoma.
    Authors: Tanaka M, Kamata R, Takigahira M, Yanagihara K, Sakai R
    Am. J. Pathol., 2007-07-01;171(1):68-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  51. Fish oil increases cholesterol storage in white adipose tissue with concomitant decreases in inflammation, hepatic steatosis, and atherosclerosis in mice.
    Authors: Saraswathi V, Gao L, Morrow JD, Chait A, Niswender KD, Hasty AH
    J. Nutr., 2007-07-01;137(7):1776-82.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  52. Cupredoxin-cancer interrelationship: azurin binding with EphB2, interference in EphB2 tyrosine phosphorylation, and inhibition of cancer growth.
    Authors: Chaudhari A, Mahfouz M, Fialho AM, Yamada T, Granja AT, Zhu Y, Hashimoto W, Schlarb-Ridley B, Cho W, Das Gupta TK, Chakrabarty AM
    Biochemistry, 2007-01-24;46(7):1799-810.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  53. EphB receptors coordinate migration and proliferation in the intestinal stem cell niche.
    Authors: Holmberg J, Genander M, Halford MM, Anneren C, Sondell M, Chumley MJ, Silvany RE, Henkemeyer M, Frisen J
    Cell, 2006-06-16;125(6):1151-63.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Immunoprecipitation
  54. EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
    Authors: Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S
    J. Cell. Sci., 2006-02-01;119(0):559-70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  55. EphB2/R-Ras signaling regulates glioma cell adhesion, growth, and invasion.
    Authors: Nakada M, Niska JA, Tran NL, McDonough WS, Berens ME
    Am. J. Pathol., 2005-08-01;167(2):565-76.
    Species: Human
    Sample Types: Cell Lysates, Tissue Homogenates, Whole Cells, Whole Tissue
    Applications: ICC, IHC-P, Immunoprecipitation, Western Blot
  56. The phosphorylation of EphB2 receptor regulates migration and invasion of human glioma cells.
    Authors: Nakada M, Niska JA, Miyamori H, McDonough WS, Wu J, Sato H, Berens ME
    Cancer Res., 2004-05-01;64(9):3179-85.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation, Neutralization, Western Blot
  57. BMPRII+ neural precursor cells isolated and characterized from organotypic neurospheres: an in vitro model of human fetal spinal cord development
    Authors: Michael W. Weible Ii, Michael D. Lovelace, Hamish D. Mundell, Tsz Wai Rosita Pang, Tailoi Chan-Ling
    Neural Regeneration Research
  58. Topographic Mapping of the Synaptic Cleft into Adhesive Nanodomains
    Authors: Karen Perez Perez de Arce, Nikolas Schrod, Sarah W. R. Metzbower, Edward Allgeyer, Geoffrey K.-W. Kong, Ai-Hui Tang et al.
    Neuron
  59. Increasing the Receptor Tyrosine Kinase EphB2 Prevents Amyloid-beta-induced Depletion of Cell Surface Glutamate Receptors by a Mechanism That Requires the PDZ-binding Motif of EphB2 and Neuronal Activity.
    Authors: Miyamoto T, Kim D, Knox J, Johnson E, Mucke L
    J Biol Chem, 2015-11-20;291(4):1719-34.
  60. Ubiquitin ligase SPSB4 diminishes cell repulsive responses mediated by EphB2
    Authors: Fumihiko Okumura, Akiko Joo-Okumura, Keisuke Obara, Alexander Petersen, Akihiko Nishikimi, Yoshinori Fukui et al.
    Molecular Biology of the Cell
  61. Aberrant EphB/ephrin-B expression in experimental gastric lesions and tumor cells
    Authors: Shintaro Uchiyama, Noritaka Saeki, Kazushige Ogawa
    World Journal of Gastroenterology
  62. Aberrant epithelial GREM1 expression initiates colonic tumorigenesis from cells outside of the crypt base stem cell niche
    Authors: Hayley Davis, Shazia Irshad, Mukesh Bansal, Hannah Rafferty, Tatjana Boitsova, Chiara Bardella et al.
    Nature Medicine

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse EphB2 Antibody

Average Rating: 4.5 (Based on 4 Reviews)

5 Star
50%
4 Star
50%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse EphB2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse EphB2 Antibody
By Anonymous on 01/17/2018
Application: WB Sample Tested: Mouse iPS Species: Mouse
Western Blot Human/Mouse EphB2 Antibody AF467
Human/Mouse EphB2 Antibody
By Li Liu on 01/12/2018
Application: Simple Western Sample Tested: Adult lung Species: Mouse
Human/Mouse EphB2 Antibody
By Dharmaraja Allimuthu on 12/21/2017
Application: WB Sample Tested: Embryonic stem cells Species: Human
Western Blot Human/Mouse EphB2 Antibody AF467
Human/Mouse EphB2 Antibody
By Anonymous on 11/10/2017
Application: WB Sample Tested: Lung tissue Species: Mouse
Western Blot Human/Mouse EphB2 Antibody AF467