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Human/Mouse FGF-23 Antibody

Catalog #: MAB2629
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2629
MAB2629-SP
Detection of Mouse FGF-23 by Western Blot
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Product Details
Citations (6)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse FGF-23 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse FGF-23 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) FGF acidic, rhFGF basic, rhFGF-3, -4, -5, -6, -7, -9, -10, -11, -12, -13, -16, -17, -18, -19, -20, -21, recombinant mouse (rm) FGF‑8b, rmFGF‑8c, or rmFGF-15.
Source
Monoclonal Rat IgG2B Clone # 283511
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FGF-23
Tyr25-Val251 (Arg179Gln)
Accession # Q9EPC2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human FGF‑23 (Catalog # 2604-FG)
Recombinant Mouse FGF‑23 (Catalog # 2629-FG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse FGF-23 by Western Blot View Larger

Detection of Mouse FGF-23 by Western Blot Effects of conditional deletion of Fgfr1 in Hyp osteocytes on gross appearance and bone-related gene expressions in 6-week-old mice.(A) Gross appearance, tail length, and body weight. Compared with control mice, Fgfr1Dmp1-cKO-null mice had normal gross appearance and body weight. However, Hyp mice showed considerably shorter tail length and lower body weight, compound Hyp;Fgfr1Dmp1-cKO-null mice displayed intermediate tail length and body weight between control and Hyp mice. Data are mean ± S.D. from 5–6 individual mice. (B and C) Western blot analysis of total Fgf2 and Fgf23 protein levels in bone. A representative Fgf2, Fgf23, and beta -Actin gel were shown in upper, middle, and lower panels of B, respectively. The intensity of bands was quantified using Image J software (https://rsb.info.nih.gov/ij/), and the data shown in C are mean ± S.D. from three independent experiments. Values sharing the same superscript in different groups are not significantly different at P<0.05. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0104154), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-23

FGF-23 is a secreted protein primarily expressed in the brain and thymus. Mutations in FGF-23 produced by osteomalacia tumors are linked to autosomal dominant hypophosphatemic rickets and several bone structure disorders.

Long Name
Fibroblast Growth Factor 23
Entrez Gene IDs
8074 (Human); 64654 (Mouse)
Alternate Names
ADHR; FGF23; FGF-23; fibroblast growth factor 23; HPDR2; HYPF; phosphatonin; PHPTC; tumor-derived hypophosphatemia inducing factor; Tumor-derived hypophosphatemia-inducing factor

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Citations for Human/Mouse FGF-23 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Twist1-Haploinsufficiency Selectively Enhances the Osteoskeletal Capacity of Mesoderm-Derived Parietal Bone Through Downregulation of Fgf23
    Authors: N Quarto, S Shailendra, NP Meyer, S Menon, A Renda, MT Longaker
    Front Physiol, 2018-10-15;9(0):1426.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. FGF23 is synthesised locally by renal tubules and activates injury-primed fibroblasts
    Authors: ER Smith, SJ Tan, SG Holt, TD Hewitson
    Sci Rep, 2017-06-13;7(1):3345.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  3. Osteocyte-specific deletion of Fgfr1 suppresses FGF23.
    Authors: Xiao Z, Huang J, Cao L, Liang Y, Han X, Quarles L
    PLoS ONE, 2014-08-04;9(8):e104154.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Fibroblast growth factor 23 concentrations in healthy term infants during the early postpartum period.
    Authors: Takaiwa M, Aya K, Miyai T
    Bone, 2010-08-01;47(2):256-62.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot
  5. Indoxyl sulfate induces left ventricular hypertrophy via the AhR-FGF23-FGFR4 signaling pathway
    Authors: Kishimoto H, Nakano T, Torisu K et al.
    Frontiers in cardiovascular medicine
  6. A computationally identified compound antagonizes excess FGF-23 signaling in renal tubules and a mouse model of hypophosphatemia
    Authors: Zhousheng Xiao, Demian Riccardi, Hector A. Velazquez, Ai L. Chin, Charles R. Yates, Jesse D. Carrick et al.
    Science Signaling

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