Human/Mouse Follistatin Antibody Summary
Gly30-Asp329
Accession # P19883
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Follistatin Inhibition of Activin A-induced Hemo-globin Expression and Neutralization by Human Follistatin Antibody. Recombinant Human Follistatin 300 (Catalog # 669-FO) inhibits Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by psuedoperoxidase activity. Inhibition of Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) activity elicited by Recombinant Human Follistatin 300 (0.4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse Follistatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF669). At 6 µg/mL, this antibody will neutralize > 60% of rhFollistatin 300 bioactivity.
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Follistatin in Human Breast. Follistatin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human/Mouse Follistatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF669) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Mouse Follistatin by Immunohistochemistry Follistatin (FST) expression is repressed in mouse mammary tumors and is restored using an Fst-expressing transgene. a Endogenous Fst expression in normal mouse mammary glands compared with human epidermal growth factor receptor 2, receptor tyrosine protein kinase erbB-2, proto-oncogene Neu (Her2/Neu)-induced mammary tumors (*p < 0.05). b Immunohistochemical analysis of endogenous FST expression in mouse mammary epithelia and tumors. cFst expression in Neu (single) and Neu/FST (bitransgenic) tumors. Fst messenger RNA expression was assessed by quantitative reverse transcription-polymerase chain reaction (**p < 0.01). d Hematoxylin and eosin (H&E)-stained mammary tumors from Neu (single) and Neu/FST (bitransgenic) mice are shown in the upper two panels. Immunohistochemistry for total (endogenous and transgenic) FST expression in lower two panels in single (left) and bitransgenic (right) tumors. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28583174), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Follistatin by Immunohistochemistry Follistatin (FST) expression is repressed in mouse mammary tumors and is restored using an Fst-expressing transgene. a Endogenous Fst expression in normal mouse mammary glands compared with human epidermal growth factor receptor 2, receptor tyrosine protein kinase erbB-2, proto-oncogene Neu (Her2/Neu)-induced mammary tumors (*p < 0.05). b Immunohistochemical analysis of endogenous FST expression in mouse mammary epithelia and tumors. cFst expression in Neu (single) and Neu/FST (bitransgenic) tumors. Fst messenger RNA expression was assessed by quantitative reverse transcription-polymerase chain reaction (**p < 0.01). d Hematoxylin and eosin (H&E)-stained mammary tumors from Neu (single) and Neu/FST (bitransgenic) mice are shown in the upper two panels. Immunohistochemistry for total (endogenous and transgenic) FST expression in lower two panels in single (left) and bitransgenic (right) tumors. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28583174), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Follistatin by Immunohistochemistry Follistatin (FST) expression is repressed in mouse mammary tumors and is restored using an Fst-expressing transgene. a Endogenous Fst expression in normal mouse mammary glands compared with human epidermal growth factor receptor 2, receptor tyrosine protein kinase erbB-2, proto-oncogene Neu (Her2/Neu)-induced mammary tumors (*p < 0.05). b Immunohistochemical analysis of endogenous FST expression in mouse mammary epithelia and tumors. cFst expression in Neu (single) and Neu/FST (bitransgenic) tumors. Fst messenger RNA expression was assessed by quantitative reverse transcription-polymerase chain reaction (**p < 0.01). d Hematoxylin and eosin (H&E)-stained mammary tumors from Neu (single) and Neu/FST (bitransgenic) mice are shown in the upper two panels. Immunohistochemistry for total (endogenous and transgenic) FST expression in lower two panels in single (left) and bitransgenic (right) tumors Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28583174), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Follistatin
Follistatin (FS) was initially identified as a follicle-stimulating hormone inhibiting substance found in ovarian follicular fluid. It has since been shown that FS is a high‑affinity activin-binding protein that can act as an activin antagonist. Two alternatively spliced follistatin mRNAs, encoding mature FS with 288 amino acid (aa) residues (FS-288) and 315 aa residues (FS-315), exist. Natural FS purified from porcine ovaries is primarily a carboxy-terminal truncated form of FS-315 composed of 300 aa residues. The recombinant human FS-300 produced at R&D Systems contains 301 aa residues and represents a molecular form derived from human FS‑315 containing a truncation of 15 residues from the carboxy–terminus. FS-288 binds with high‑affinity to cell-surface heparan sulfate proteoglycans whereas FS-315 binds with low-affinity. The binding affinity of R&D Systems’ FS-300 to heparan sulfate has not been determined. Cell surface-associated FS has been suggested to play a role in the clearance and bioavailability of activin in vivo. Besides activin, FS has also been shown to bind with multiple BMPs and to inhibit BMP activity in early Xenopus embryos. FS deficient mice have been shown to have multiple embryonic defects that will result in death shortly after birth. Overexpression of FS can also cause reproductive defects in transgenic mice. Over aa 30-329, human Follistatin shares 97% aa identity with mouse Follistatin.
Product Datasheets
Citations for Human/Mouse Follistatin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
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Taste papilla cell differentiation requires tongue mesenchyme via ALK3-BMP signaling to regulate the production of secretory proteins
Authors: M Ishan, Z Wang, P Zhao, Y Yao, S Stice, L Wells, Y Mishina, HX Liu
bioRxiv : the preprint server for biology, 2023-04-04;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction
Authors: Salemi S, Schori L, Gerwinn T et al.
International Journal of Molecular Sciences
Species: Human
Sample Types: Protein
Applications: Simple Western -
Single-cell analysis of early chick hypothalamic development reveals that hypothalamic cells are induced from prethalamic-like progenitors
Authors: Dong Won Kim, Elsie Place, Kavitha Chinnaiya, Elizabeth Manning, Changyu Sun, Weina Dai et al.
Cell Reports
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Gene therapy for follistatin mitigates systemic metabolic inflammation and post-traumatic arthritis in high-fat diet-induced obesity
Authors: R Tang, NS Harasymowi, CL Wu, KH Collins, YR Choi, SJ Oswald, F Guilak
Sci Adv, 2020-05-08;6(19):eaaz7492.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Myostatin and activin blockade by engineered follistatin results in hypertrophy and improves dystrophic pathology in mdx mouse more than myostatin blockade alone
Authors: A Iskenderia, N Liu, Q Deng, Y Huang, C Shen, K Palmieri, R Crooker, D Lundberg, N Kastrapeli, B Pescatore, A Romashko, J Dumas, R Comeau, A Norton, J Pan, H Rong, K Derakhchan, DE Ehmann
Skelet Muscle, 2018-10-27;8(1):34.
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Protein Engineering on Human Recombinant Follistatin: Enhancing Pharmacokinetic Characteristics for Therapeutic Application
Authors: Chuan Shen, Andrea Iskenderian, Dianna Lundberg, Tao He, Kathleen Palmieri, Robert Crooker et al.
Journal of Pharmacology and Experimental Therapeutics
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Inhibition of the Activin Receptor Type-2B Pathway Restores Regenerative Capacity in Satellite Cell-Depleted Skeletal Muscle
Authors: L Formicola, A Pannérec, RM Correra, B Gayraud-Mo, D Ollitrault, V Besson, S Tajbakhsh, J Lachey, JS Seehra, G Marazzi, DA Sassoon
Front Physiol, 2018-05-24;9(0):515.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Transcriptomic analysis comparing mouse strains with extreme total lung capacities identifies novel candidate genes for pulmonary function
Authors: L George, A Mitra, TA Thimraj, M Irmler, S Vishweswar, L Lunding, D Hühn, A Madurga, J Beckers, H Fehrenbach, S Upadhyay, H Schulz, GD Leikauf, K Ganguly
Respir. Res., 2017-08-09;18(1):152.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Follistatin is a metastasis suppressor in a mouse model of HER2-positive breast cancer
Authors: DD Seachrist, ST Sizemore, E Johnson, FW Abdul-Kari, KL Weber Bonk, RA Keri
Breast Cancer Res., 2017-06-05;19(1):66.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Differential Expression and Release of Activin A and Follistatin in Chronic Rhinosinusitis with and without Nasal Polyps.
Authors: Yang Y, Zhang N, Crombruggen K, Lan F, Hu G, Hong S, Bachert C
PLoS ONE, 2015-06-01;10(6):e0128564.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Dendritic cell regulation of NK-cell responses involves lymphotoxin-alpha, IL-12, and TGF-beta.
Authors: Sarhan D, Palma M, Mao Y, Adamson L, Kiessling R, Mellstedt H, Osterborg A, Lundqvist A
Eur J Immunol, 2015-04-17;45(6):1783-93.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Combinatory effects of siRNA-induced myostatin inhibition and exercise on skeletal muscle homeostasis and body composition
Authors: Stephanie Mosler, Karima Relizani, Etienne Mouisel, Helge Amthor, Patrick Diel
Physiological Reports
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Analysis of the effects of androgens and training on myostatin propeptide and follistatin concentrations in blood and skeletal muscle using highly sensitive Immuno PCR.
Authors: Diel P, Schiffer T, Geisler S, Hertrampf T, Mosler S, Schulz S, Wintgens KF, Adler M
Mol. Cell. Endocrinol., 2010-08-27;330(1):1-9.
Species: Human
Sample Types: Recombinant Protein, Serum
Applications: ELISA Development, Immuno-PCR, Western Blot -
Follistatin induction by nitric oxide through cyclic GMP: a tightly regulated signaling pathway that controls myoblast fusion
Authors: Addolorata Pisconti, Silvia Brunelli, Monica Di Padova, Clara De Palma, Daniela Deponti, Silvia Baesso et al.
The Journal of Cell Biology
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Follistatin restricts bone morphogenetic protein (BMP)-2 action on the differentiation of osteoblasts in fetal rat mandibular cells.
Authors: Abe Y, Abe T, Aida Y, Hara Y, Maeda K
J. Bone Miner. Res., 2004-05-03;19(8):1302-7.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization
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incubated 1hr at 37C. diluted in reagent diluent 2
