Human/Mouse FoxP3 Antibody

Catalog #: FPK8214A Datasheet / COA / SDS

Recombinant Monoclonal Antibody. A flow cytometry staining kit containing an APC-conjugated FoxP3 monoclonal antibody (IC8214A) and FoxP3 Fixation and Permeabilization Buffers (FC012)

Catalog #	Availability	Size / Price	Qty
FPK8214A-025
FPK8214A-100

Detection of FoxP3 in Human PBMC Natural Tregs by Flow Cytometry.

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Human/Mouse FoxP3 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse FoxP3 in flow cytometry.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1054C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human FoxP3
Met1-Leu71

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of FoxP3 in Human PBMC Natural Tregs by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) were surface stained with (A) Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F) and (B) Mouse Anti-Human IL-2 Ra (CD25) PE-conjugated Monoclonal Antibody (Catalog # FAB1020P), followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 APC-conjugated Monoclonal Antibody (Catalog # IC8214A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes

Flow Cytometry

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Detection of FoxP3 in C57BL/6 Mouse Splenocyte Natural Tregs by Flow Cytometry. C57BL/6 mouse splenocyte natural regulatory T cells (Tregs) were surface stained with (A) Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and (B) Rat Anti-Mouse IL-2 Ra (CD25) PE-conjugated Monoclonal Antibody (Catalog # FAB2438P) followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 APC-conjugated Monoclonal Antibody (Catalog # IC8214A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 to 8 °C. Do not use past expiration date. Protect from light.

Background: FoxP3

Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4⁺ T cells. In human, FoxP3 is found in CD4⁺, CD8⁺ and CD4⁺CD25⁺ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.

Long Name

Forkhead Box P3

Entrez Gene IDs

50943 (Human); 20371 (Mouse)

Alternate Names

AIID; AIIDMGC141961; DIETER; forkhead box P3; Forkhead Box Protein P3; FoxP3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; IPEX; JM2; MGC141961; MGC141963; PIDX; PIDXMGC141963; SCURFIN; XPID; XPIDpolyendocrinopathy, enteropathy, X-linked