Human/Mouse Frizzled-2 Antibody Summary
Gln29-Leu168
Accession # Q9JIP6
Applications
Gujral, T. et al. (2014) Cell 159:844.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Frizzled-2 by Western Blot Small intestinal epithelial cells of Plcb3−/− mice exhibit downregulation of the Wnt/ beta -catenin signaling pathway. (A) GSEA indicates that the gene expression pattern in isolated IECs from Plcb3−/− mice correlates with the reduced expression of Wnt/ beta -catenin signature genes (upper left), Lgr4 regulated signature genes (lower left), Lgr4/Lgr5 regulated signature genes (upper right), and Ascl2 signature genes (lower right). NES: normalized enrichment score. n = 3 biological replicates. (B) qRT-PCR analysis of isolated IECs from WT and Plcb3−/− mice exposed to sterilized water or 3% DSS for 2 days. Normalized against Hprt mRNA. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001, determined by two-tailed Student’s t-test. (C) GSEA with isolated IECs from Plcb3 ∆IEC mice indicated the reduced expression of Wnt/ beta -catenin (upper left), Lgr4 (lower left), Lgr4/Lgr5 (upper right), and Ascl2 (lower right) signature genes at the homeostatic condition. (D) Small intestines of WT and Plcb3−/− mice exposed to sterilized water or 3% DSS for 2 days were separated into mesenchymal tissues. RNAs isolated were subjected to qRT-PCR analysis. mRNA amounts for several Wnt ligands and Wnt secretion enablers (Wls and Porcn) were normalized against Hprt. * p < 0.05; ** p < 0.01, determined by Student’s t-test. (E) Western blot analysis of Wnt/ beta -catenin signaling proteins from WT and Plcb3−/− mice (3 mice each) under H2O and 2-day DSS treatments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38891118), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frizzled-2
Wnt signaling is involved in variety of developmental processes including cell fate determination, cell polarity, tissue patterning and control of cell proliferation. Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail. One of the most conserved regions of the Frizzled proteins is the extracellular cysteine-rich domain (CRD) which spans approximately 120 AA and contains 10 invariant cysteines (1). Mouse Frizzled-2 shows 100% amino acid identity to human and rat Frizzled-2 in the CRD region. Frizzled-2 expression is greater in embryonic than adult tissues, with heart, brain, lung, kidney and gut showing the highest levels (2). In addition, Frizzled-2 is expressed during migration and proliferation of neural crest cells that populate the heart and great arteries (3). A non-canonical Frizzled-2 pathway has been shown to regulate Epithelial-Mesenchymal Transition (EMT) and metastasis (4). Secreted frizzled related proteins (sFRPs), antagonists of the Wnt pathway, have been shown to interact with both Wnt ligands and Frizzled receptors (5).
- Wang, Y. et al. (1996) J. Biol. Chem 271:4468.
- Malik, T. and R. Shivdasani (2000) Biochem. J. 349:829.
- van Gijn, M. et al. (2001) Anat. Embryol. 203:185.
- Gujral, T. et al. (2014) Cell 159:844.
- Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
Product Datasheets
Citations for Human/Mouse Frizzled-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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A novel and robust conditioning lesion induced by ethidium bromide
Authors: Edmund R. Hollis, Nao Ishiko, Kristine Tolentino, Ernest Doherty, Maria J. Rodriguez, Nigel A. Calcutt et al.
Experimental Neurology
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Ileal Crohn's Disease Exhibits Reduced Activity of Phospholipase C-?3-Dependent Wnt/?-Catenin Signaling Pathway
Authors: Ando, T;Takazawa, I;Spencer, ZT;Ito, R;Tomimori, Y;Mikulski, Z;Matsumoto, K;Ishitani, T;Denson, LA;Kawakami, Y;Kawakami, Y;Kitaura, J;Ahmed, Y;Kawakami, T;
Cells
Species: Mouse, Transgenic Mouse
Sample Types: Tissue Homogenates, Cell Lysates
Applications: Western Blot -
Bioengineered BERA-Wnt5a siRNA targeting Wnt5a/FZD2 signaling suppresses advanced prostate cancer tumor growth and enhances enzalutamide treatment
Authors: AC Gao, S Ning, C Liu, W Lou, JC Yang, AP Lombard, LS D'Abronzo, N Batra, AM Yu, AR Leslie, M Sharifi, CP Evans
Molecular Cancer Therapeutics, 2022-10-07;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Stably overexpressed human Frizzled-2 signals through the beta-catenin pathway and does not activate Ca2+-mobilization in Human Embryonic Kidney 293 cells.
Authors: Verkaar F, van Rosmalen JW, Smits JF, Blankesteijn WM, Zaman GJ
Cell. Signal., 2008-09-25;21(1):22-33.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development
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