Human/Mouse Frizzled-3 Antibody Summary
Met1-Val157
Accession # Q61086
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Frizzled‑3 in Mouse Cortex. Frizzled‑3 was detected in immersion fixed paraffin-embedded sections of mouse cortex using Rat Anti-Human/Mouse Frizzled‑3 Monoclonal Antibody (Catalog # MAB1001) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Frizzled‑3 in Human Cortex. Frizzled‑3 was detected in immersion fixed paraffin-embedded sections of human cortex using Rat Anti-Human/Mouse Frizzled‑3 Monoclonal Antibody (Catalog # MAB1001) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frizzled-3
Frizzled-3 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.
Product Datasheets
Citations for Human/Mouse Frizzled-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Clinical significance of frizzled homolog 3 protein in colorectal cancer patients.
Authors: Wong, Sze Chue, He, Catherin, Chan, Charles, Chan, Amanda K, Wong, Heong Ti, Cheung, Moon Ton, Luk, Lewis La, Au, Thomas C, Chiu, Man Kin, Ma, Brigette, Chan, Anthony
PLoS ONE, 2013-11-08;8(11):e79481.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Aberrant planar cell polarity induced by urinary tract obstruction.
Authors: Li L, Zepeda-Orozco D, Patel V
Am. J. Physiol. Renal Physiol., 2009-09-30;297(6):F1526-33.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot -
Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation.
Authors: Baksh D, Boland GM, Tuan RS
J. Cell. Biochem., 2007-08-01;101(5):1109-24.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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