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Human/Mouse Frizzled-4 Biotinylated Antibody

Catalog #: BAM194
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BAM194
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Human/Mouse Frizzled-4 Biotinylated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse Frizzled-1, -2, -3, -6, -7, or -8 is observed. In flow cytometry and immunohistochemistry, this antibody also detected Frizzled-4 on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3, and also on human HEK293 cells.
Source
Monoclonal Rat IgG2A Clone # 145901
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4
Met1-Glu180
Accession # Q61088
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Endotoxin Level
<0.15 EU per 1 μg of the antibody by the LAL method.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Frizzled-4 (Catalog # 5847-FZ)
Recombinant Mouse Frizzled-4 Fc Chimera (Catalog # 194-FZ)
Flow Cytometry
0.25 µg/106 cells
HEK293 human embryonic kidney cell line
Immunocytochemistry
8-25 µg/mL
Immersion fixed HEK293 human embryonic kidney cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-4

Frizzled-4 belongs to the Frizzled family of 7-pass transmembrane proteins and binds to Wnt-8. It is implicated in several developmental processes, notably in retinal angiogenesis.

Entrez Gene IDs
8322 (Human); 14366 (Mouse)
Alternate Names
CD344 antigen; CD344; EVR1; exudative vitreoretinopathy 1; FEVR; frizzled (Drosophila) homolog 4; frizzled homolog 4 (Drosophila); Frizzled4; Frizzled-4; Fz-4; FZD4; FZD4S; FzE4; GPCR; hFz4; MGC34390; WNT receptor frizzled-4

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Citation for Human/Mouse Frizzled-4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Focal adhesion kinase splice variants maintain primitive acute myeloid leukemia cells through altered wnt signaling.
    Authors: Despeaux M, Chicanne G, Rouer E, De Toni-Costes F, Bertrand J, Mansat-De Mas V, Vergnolle N, Eaves C, Payrastre B, Girault JA, Racaud-Sultan C
    Stem Cells, 2012-08-01;30(8):1597-610.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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