Human/Mouse GFR alpha-2/GDNF R alpha-2 Antibody

Name: Human/Mouse GFR alpha-2/GDNF R alpha-2 Antibody
Brand: R&D Systems
SKU: AF429
Rating: 5 (2 reviews)

Catalog #: AF429
25 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF429
AF429-SP

GFR alpha ‑2/GDNF R alpha ‑2 in Mouse Splenocytes.

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All GFR alpha-2/GDNF R alpha-2 Antibodies

Product Details

Citations (25)

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Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse GFR alpha-2/GDNF R alpha-2 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse GFR alpha ‑2/GDNF R alpha ‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
5% cross‑reactivity with recombinant human GFR alpha -3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse GFR alpha ‑2/GDNF R alpha ‑2
Ser22-Ser441
Accession # Q3UUD8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse GFR alpha ‑2/GDNF R alpha ‑2 Fc Chimera (Catalog # 429-FR)

Immunohistochemistry

5-15 µg/mL

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 2 ng/mL of Recombinant Human GDNF (Catalog # 212-GD) to immobilized Recombinant Mouse GFR alpha -2 Fc Chimera (Catalog # 429-FR) coated at 1 µg/mL (100 µL/well). At 15 μg/mL, this antibody will block >90% of the binding.

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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GFR alpha ‑2/GDNF R alpha ‑2 in Mouse Splenocytes. GFRa-2/GDNF Ra-2 was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse GFRa-2/GDNF Ra-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF429) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry

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GFR alpha ‑2/GDNF R alpha ‑2 in Mouse Dorsal Root Ganglion. GFRa-2/GDNF Ra-2 was detected in perfusion fixed frozen sections of mouse dorsal root ganglion using Goat Anti-Human/Mouse GFRa-2/GDNF Ra-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF429) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane of sensory neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-2/GDNF R alpha-2

Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PSP) and artemin, distant members of the TGF-beta superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits, GDNF receptor alpha -1 (GFR alpha -1, also known as Trn R1) or GFR alpha -2 (also known as Trn R2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF or NTN to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase.

Mouse GFR alpha -2 cDNA encodes a 463 amino acid (aa) residue protein with a putative N-terminal 21 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -2 has a C-terminal hydrophobic region which is preceded by a 3 aa residue (SGS) GPI-binding site. Human GFR alpha -2 shares 96.5% amino acid identity with mouse GFR alpha -2. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

References

Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
Baloh, R.H. et al. (1998) Neuron 21:1291.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha 2

Entrez Gene IDs

2675 (Human); 14586 (Mouse)

Alternate Names

GDNF family receptor alpha 2; GDNF family receptor alpha-2; GDNF R alpha-2; GDNF receptor alpha-2; GDNF receptor beta; GDNFR-alpha-2; GDNFR-beta; GDNFRBRET ligand 2; GFR alpha2; GFR alpha-2; GFRA2; GFRa-2; GFR-alpha 2; GFR-alpha-2; glial cell line derived neurotrophic factor receptor, beta; Neurturin receptor alpha; NRTNR-alpha; NTNRA; NTNR-alpha; PI-linked cell-surface accessory protein; RETL2TGF-beta-related neurotrophic factor receptor 2; TGF-beta related neurotrophic factor receptor 2; TRN receptor, GPI-anchored; TRNR2NRTNR-ALPHA

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

GDNF Family

Citations for Human/Mouse GFR alpha-2/GDNF R alpha-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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Expression of Glial Cell‐Derived Neurotrophic Factor Receptors Within Nucleus Ambiguus During Rat Development
Authors: Quinton Blount, Ignacio Hernandez‐Morato, Yalda Moayedi, Michael J. Pitman
The Laryngoscope
Cutaneous TRPV1+ Neurons Trigger Protective Innate Type 17 Anticipatory Immunity
Authors: Jonathan A. Cohen, Tara N. Edwards, Andrew W. Liu, Toshiro Hirai, Marsha Ritter Jones, Jianing Wu et al.
Cell
Sciatic nerve injury in adult rats causes distinct changes in the central projections of sensory neurons expressing different glial cell line-derived neurotrophic factor family receptors
Authors: Janet R. Keast, Shelley L. Forrest, Peregrine B. Osborne
The Journal of Comparative Neurology
Elevated dietary omega -6 polyunsaturated fatty acids induce reversible peripheral nerve dysfunction that exacerbates comorbid pain conditions
Authors: Jacob T. Boyd, Peter M. LoCoco, Ashley R. Furr, Michelle R. Bendele, Meilinn Tram, Qun Li et al.
Nature Metabolism
A cholinergic neuroskeletal interface promotes bone formation during postnatal growth and exercise
Authors: Stephen Gadomski, Claire Fielding, Andrés García-García, Claudia Korn, Chrysa Kapeni, Sadaf Ashraf et al.
Cell Stem Cell
Gdnf, Neurturin and Artemin Activate and Sensitize Bone Afferent Neurons and Contribute To Inflammatory Bone Pain
Authors: S Nencini, M Ringuet, DH Kim, C Greenhill, JJ Ivanusic
J. Neurosci., 2018-04-30;0(0):.
Peripheral injury of pelvic visceral sensory nerves alters GFRÎ± (GDNF family receptor alpha) localization in sensory and autonomic pathways of the sacral spinal cord
Authors: Shelley L. Forrest, Sophie C. Payne, Janet R. Keast, Peregrine B. Osborne
Frontiers in Neuroanatomy
Return of the Tbx5; lineage-tracing reveals ventricular cardiomyocyte-like precursors in the injured adult mammalian heart
Authors: Panagiota Siatra, Giannis Vatsellas, Athanasia Chatzianastasiou, Evangelos Balafas, Theodora Manolakou, Andreas Papapetropoulos et al.
npj Regenerative Medicine
Long-term functional regeneration of radiation-damaged salivary glands through delivery of a neurogenic hydrogel
Authors: J Li, S Sudiwala, L Berthoin, S Mohabbat, EA Gaylord, H Sinada, N Cruz Pache, JC Chang, O Jeon, IMA Lombaert, AJ May, E Alsberg, CS Bahney, SM Knox
Science Advances, 2022-12-21;8(51):eadc8753.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC/IF
Cholinergic signals preserve haematopoietic stem cell quiescence during regenerative haematopoiesis
Authors: C Fielding, A García-Gar, C Korn, S Gadomski, Z Fang, JL Reguera, JA Pérez-Simó, B Göttgens, S Méndez-Fer
Nature Communications, 2022-01-27;13(1):543.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC

Gut-innervating TRPV1+ neurons drive chronic visceral pain via microglial P2Y12 receptor
Authors: M Defaye, NS Abdullah, M Iftinca, A Hassan, F Agosti, Z Zhang, M Cumenal, GW Zamponi, C Altier
Cellular and Molecular Gastroenterology and Hepatology, 2021-12-24;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

ARID1a Associates with Lymphoid-Restricted Transcription Factors and Has an Essential Role in T Cell Development
Authors: A Astori, J Tingvall-G, J Kuruvilla, E Coyaud, EMN Laurent, M Sunnerhage, J Åhsberg, J Ungerbäck, T Strid, M Sigvardsso, B Raught, R Somasundar
J. Immunol., 2020-08-03;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Salivary glands regenerate after radiation injury through SOX2-mediated secretory cell replacement
Authors: E Emmerson, AJ May, L Berthoin, N Cruz-Pache, S Nathan, AJ Mattingly, JL Chang, WR Ryan, AD Tward, SM Knox
EMBO Mol Med, 2018-03-01;0(0):.
Species: Human, Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Neurturin Gene Therapy Protects Parasympathetic Function to Prevent Irradiation-Induced Murine Salivary Gland Hypofunction
Authors: JNA Ferreira, C Zheng, IMA Lombaert, CM Goldsmith, AP Cotrim, JM Symonds, VN Patel, MP Hoffman
Mol Ther Methods Clin Dev, 2018-02-23;9(0):172-180.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Protection of nigral dopaminergic neurons by AAV1 transduction with Rheb(S16H) against neurotoxic inflammation in vivo
Authors: S Kim, GJ Moon, YS Oh, J Park, WH Shin, JY Jeong, KS Choi, BK Jin, N Kholodilov, RE Burke, HJ Kim, CM Ha, SG Lee, SR Kim
Exp. Mol. Med., 2018-02-09;50(2):e440.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Neurturin and a Glp-1 Analogue Act Synergistically to Alleviate Diabetes in Zucker Diabetic Fatty Rats
Authors: JL Trevaskis, CB Sacramento, H Jouihan, S Ali, J Le Lay, S Oldham, N Bhagroo, BB Boland, J Cann, Y Chang, T O'Day, V Howard, C Reers, MS Winzell, DM Smith, M Feigh, P Barkholt, K Schreiter, M Austen, U Andag, S Thompson, L Jermutus, MP Coghlan, J Grimsby, C Dohrmann, CJ Rhodes, CM Rondinone, A Sharma
Diabetes, 2017-04-13;0(0):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC

Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
Nat Neurosci, 2014-11-24;18(1):145-53.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Parasympathetic stimulation improves epithelial organ regeneration.
Authors: Knox, Sarah M, Lombaert, Isabelle, Haddox, Candace, Abrams, Shaun R, Cotrim, Ana, Wilson, Adrian J, Hoffman, Matthew
Nat Commun, 2013-01-01;4(0):1494.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo.
Authors: Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM
J. Neurosci., 2006-08-16;26(33):8588-99.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr

Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats.
Authors: Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O
Stroke, 2006-07-27;37(9):2361-7.
Species: Rat
Sample Types: Whole Cells
Applications: ICC

Expression and function of GDNF family ligands and receptors in the carotid body.
Authors: Leitner ML, Wang LH, Osborne PA, Golden JP, Milbrandt J, Johnson EM
Exp. Neurol., 2005-02-01;191(0):S68-79.
Species: Rat
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC

Neurotrophin and GDNF family ligands promote survival and alter excitotoxic vulnerability of neurons derived from murine embryonic stem cells.
Authors: Lee CS, Tee LY, Dusenbery S, Takata T, Golden JP, Pierchala BA, Gottlieb DI, Johnson EM, Choi DW, Snider BJ
Exp. Neurol., 2005-01-01;191(1):65-76.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

GDNF family receptors in the embryonic and postnatal rat heart and reduced cholinergic innervation in mice hearts lacking ret or GFRalpha2.
Authors: Hiltunen JO, Laurikainen A, Airaksinen MS, Saarma M
Dev. Dyn., 2000-09-01;219(1):28-39.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Genetic interplay between transcription factor Pou4f1/Brn3a and neurotrophin receptor Ret in retinal ganglion cell type specification
Authors: Vladimir Vladimirovich Muzyka, Tudor Constantin Badea
Neural Development

Characterization of bladder sensory neurons in the context of myelination, receptors for pain modulators, and acute responses to bladder inflammation
Authors: Shelley L. Forrest, Peregrine B. Osborne, Janet R. Keast
Frontiers in Neuroscience