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Human/Mouse GLI-1 Biotinylated Antibody

Catalog #: BAF3455
1 Citation Datasheet / COA / SDS
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BAF3455
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Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse GLI-1 Biotinylated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse GLI-1 in Western blot. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) GLI-2 and rmGLI-3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse GLI-1
Met1-Glu237
Accession # P47806
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human GLI‑1
Recombinant Mouse GLI‑1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLI-1

GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI-1 activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.

Long Name
Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]
Entrez Gene IDs
2735 (Human); 14632 (Mouse); 140589 (Rat)
Alternate Names
GLI family zinc finger 1; GLI1; GLI-1; glioma-associated oncogene family zinc finger 1; glioma-associated oncogene homolog 1 (zinc finger protein); Glioma-associated oncogene; GLIzinc finger protein GLI1; Oncogene GLI

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Citation for Human/Mouse GLI-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A conserved HH-Gli1-Mycn network regulates heart regeneration from newt to human
    Authors: BN Singh, N Koyano-Nak, W Gong, IP Moskowitz, CV Weaver, E Braunlin, S Das, JH van Berlo, MG Garry, DJ Garry
    Nat Commun, 2018-10-12;9(1):4237.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP

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