Home / GLI-3 / Human/Mouse GLI-3 Antibody

Human/Mouse GLI-3 Antibody

Catalog #: AF3690
101 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3690
AF3690-SP
Detection of Mouse GLI‑3 by Western Blot.
5 Images
Product Details
Citations (101)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse GLI-3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse GLI-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GLI-3
Met1-Glu479
Accession # P10071
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse GLI‑3 antibody by Western Blot. View Larger

Detection of Mouse GLI‑3 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GLI-3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Chromatin Immunoprecipitation (ChIP) Detection of GLI-3-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of GLI‑3-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-3/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thegli-1promoter was detected by standard PCR.

Immunocytochemistry GLI-3 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

GLI‑3 in HeLa Human Cell Line. GLI-3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Detection of Mouse GLI-3 by Western Blot View Larger

Detection of Mouse GLI-3 by Western Blot Conditional deletion of GATA6 does not significantly alter Gli3 processing in the hindlimb bud.(A) Western blot analysis of Gli3FL and Gli3R steady state levels in anterior (lanes 1 and 3) or posterior (lanes 2 and 4) halves of E11.5 hindlimb buds isolated from either control (lanes 1 and 2) or Prx1-Cre; GATA6fl/fl (lanes 3 and 4) mouse embryos. alpha –Tubulin is used as a loading control. (B) Quantification of Gli3FL/Gli3R ratios (by densitometry of Western Blots) in anterior or posterior halves of E11.5 hindlimb buds isolated from either littermate controls (lanes 1 and 2) or Prx1-Cre; GATA6fl/fl (lanes 3 and 4) animals, 12 embryos of each genotype were analyzed. Error bar indicates standard deviation, n = 12. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24415953), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse GLI-3 by Western Blot View Larger

Detection of Mouse GLI-3 by Western Blot SufuT396I does not stabilize Gli3FL protein and reduces the processing of Gli3FL.(A) Western blotting of lysates prepared from SufuR146X/R146X at E9.5 and SufuT396I/T396I, SufuT396I/+, and wild-type embryos at E10.5 with anti-Gli3, anti-Sufu, and anti-actin antibodies. Each image presented in the Fig. is a representative of independent triplicated experiments. The full gel images are shown in S6A Fig. (B, C) Relative expression of Gli3FL (B) and Gli3REP (B and C). Western blotting was performed two times using lysates prepared from five wild-type and five SufuT396I/T396I embryos at E10.5 (S2A Fig.). Expression levels were quantified from the band intensity shown in S2A Fig. as relative values of the Gli3FL/actin and Gli3REP/actin expression ratios (B) and the direct ratio of Gli3REP/Gli3FL (C). (**) p < 0.01, two-tailed Student’s t-test. Error bars indicate the standard deviations. (D) Western blotting of cell lysates from wild-type and SufuT396I/T396I MEFs with indicated antibodies. The SufuT396I/T396I MEFs were electroporated with 10.0, 1.0, or 0.1 μg of the HA–Sufu construct (lane 3, 4, and 5, respectively), or 10 μg of the HA–SufuT396I construct (lane 6). The mobilities on SDS-PAGE of the wild-type Sufu (lane 3–5) and SufuT396I (lane 6) are identical. The complete gel images are shown in S6B Fig. This image is representative of two independent experiments. (E) Western blotting of cell lysates from Sufu−/− cells with indicated antibodies. The Sufu−/− cells were electroporated with a mixture of the Flag–Gli3 construct (6 μg) and the HA–Sufu construct (4.00, 1.33, 0.44, or 0.15 μg) for the wild-type Sufu cotransfection (lane 2 to 5, respectively), or a mixture of the Flag–Gli3 construct (6 μg) and the HA–SufuT396I construct (4 μg) for mutant Sufu cotransfection (lane 6). The complete gel images are shown in S6C Fig. This image is representative of two independent experiments. (F) Western blotting of immunoprecipitates or lysates from 293T cells transfected with expression constructs as indicated at the top. The complete gel images are shown in S6D Fig. This image is representative of two independent experiments. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25760946), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLI-3

Human GLI-3 is a 190 kDa transcription factor of the C2H2-type zinc-finger protein family. GLI-3 appears to play a role in limb and brain development, probably through sonic hedgehog signaling. GLI-3 is regulated by PKA-mediated phosphorylation. Unphosphorylated full-length GLI-3 is a transcriptional activator, while phosphorylated GLI-3 is cleaved after amino acid 700 to generate an N-terminal 83 kDa transcriptional repressor. Over the region used as immunogen, human GLI-3 is 95% identical to the corresponding mouse and canine protein sequences.

Long Name
GLI-Kruppel family member GLI3
Entrez Gene IDs
2737 (Human); 14634 (Mouse); 140588 (Rat)
Alternate Names
ACLS; ACLSPAPAPHS; ADD; Bph; GCPS; GLI family zinc finger 3; GLI3 form of 190 kDa; GLI3 full length protein; GLI3; GLI-3; GLI3-190; GLI3FL; GLI-Kruppel family member GLI3; glioma-associated oncogene family zinc finger 3; Greig cephalopolysyndactyly syndrome; oncogene GLI3; PAP-A; PAPA1; PAPB; PAPBDNA-binding protein; Pdn; PHS; PPDIV; Xt; zinc finger protein GLI3

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human/Mouse GLI-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

101 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Tissue microarray analysis indicates hedgehog signaling as a potential prognostic factor in intermediate-risk prostate cancer
    Authors: Gonnissen A, Isebaert S, Perneel C et al.
    BMC Cancer
  2. Temporal transcriptome features identify early skeletal commitment during human epiphysis development at single-cell resolution
    Authors: Zhonghao Deng, Shengwei Rong, Lu Gan, Fuhua Wang, Liangxiao Bao, Fang Cai et al.
    iScience
  3. The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium
    Authors: Gerhardt C, Lier JM, Burmuhl S et al.
    J. Cell Biol.
  4. Mutations in DYNC2LI1 disrupt cilia function and cause short rib polydactyly syndrome.
    Authors: Taylor SP, Dantas TJ, Duran I et al.
    Nat Commun
  5. Vertebrate cells differentially interpret ciliary and extraciliary cAMP
    Authors: Truong ME, Bilekova S, Choksi SP et al.
    Cell
  6. Itch/?-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis
    Authors: P Infante, R Faedda, F Bernardi, F Bufalieri, L Lospinoso, R Alfonsi, D Mazzà, M Siler, S Coni, A Po, M Petroni, E Ferretti, M Mori, E De Smaele, G Canettieri, C Capalbo, M Maroder, I Screpanti, M Kool, SM Pfister, D Guardavacc, A Gulino, L Di Marcotu
    Nat Commun, 2018-03-07;9(1):976.
  7. The Hedgehog/GLI signaling pathway activates transcription of Slug (Snail2) in melanoma cells
    Authors: P Horák, K Kreisinger, J Réda, L Ondrušová, J Balko, J Vachtenhei, P Žáková, J Vachtenhei
    Oncology reports, 2023-03-03;49(4):.
  8. PP2A phosphatase regulatory subunit PPP2R3C is a new positive regulator of the hedgehog signaling pathway
    Authors: Baran, B;Derua, R;Janssens, V;Niewiadomski, P;
    Cellular signalling
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Targeted protein degradation reveals BET bromodomains as the cellular target of Hedgehog pathway inhibitor-1
    Authors: Meropi Bagka, Hyeonyi Choi, Margaux Héritier, Hanna Schwaemmle, Quentin T. L. Pasquer, Simon M. G. Braun et al.
    Nature Communications
  10. Inferring and perturbing cell fate regulomes in human brain organoids
    Authors: JS Fleck, SMJ Jansen, D Wollny, F Zenk, M Seimiya, A Jain, R Okamoto, M Santel, Z He, JG Camp, B Treutlein
    Nature, 2022-10-05;0(0):.
    Species: Human
    Sample Types: Organoid, Tissue Homogenates, Whole Cells
    Applications: Bioassay, CUT&amp;Tag, IHC
  11. RNA-seq and ChIP-seq Identification of Unique and Overlapping Targets of GLI Transcription Factors in Melanoma Cell Lines
    Authors: M Kurtovi?, N Piteša, N Bartoni?ek, P Ozreti?, V Musani, J ?onkaš, T Petri?, C King, M Sabol
    Cancers, 2022-09-19;14(18):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  12. Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages
    Authors: Megan Rowton, Carlos Perez-Cervantes, Suzy Hur, Jessica Jacobs-Li, Emery Lu, Nikita Deng et al.
    Developmental Cell
  13. Sonic hedgehog is not a limb morphogen but acts as a trigger to specify all digits in mice
    Authors: Jianjian Zhu, Rashmi Patel, Anna Trofka, Brian D. Harfe, Susan Mackem
    Developmental Cell
  14. Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease
    Authors: S Schmidt, MD Luecken, D Trümbach, S Hembach, KM Niedermeie, N Wenck, K Pflügler, C Stautner, A Böttcher, H Lickert, C Ramirez-Su, R Ahmad, MJ Ziller, JC Fitzgerald, V Ruf, WDJ van de Ber, AJ Jonker, T Gasser, B Winner, J Winkler, DM Vogt Weise, F Giesert, FJ Theis, W Wurst
    Nature Communications, 2022-08-16;13(1):4819.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  15. The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling
    Authors: Leah Schembs, Ariane Willems, Kerstin Hasenpusch-Theil, James D. Cooper, Katie Whiting, Karen Burr et al.
    Cell Reports
  16. Ptch2 is a Potential Regulator of Mesenchymal Stem Cells
    Authors: Juuri E, Tikka P, Domanskyi A et al.
    Frontiers in Physiology
  17. Phylogenetic profiling and cellular analyses of ARL16 reveal roles in traffic of IFT140 and INPP5E
    Authors: Skylar I. Dewees, Romana Vargová, Katherine R. Hardin, Rachel E. Turn, Saroja Devi, Joshua Linnert et al.
    Molecular Biology of the Cell
  18. Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development
    Authors: J Sun, J Yoon, M Lee, HK Lee, YS Hwang, IO Daar
    Cell Reports, 2022-02-01;38(5):110312.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  19. The ARF GAPs ELMOD1 and ELMOD3 act at the Golgi and cilia to regulate ciliogenesis and ciliary protein traffic
    Authors: Rachel E. Turn, Yihan Hu, Skylar I. Dewees, Narra Devi, Michael P. East, Katherine R. Hardin et al.
    Molecular Biology of the Cell
  20. HES1 is a novel downstream modifier of the SHH-GLI3 Axis in the development of preaxial polydactyly
    Authors: D Sharma, AJ Mirando, A Leinroth, JT Long, CM Karner, MJ Hilton
    PloS Genetics, 2021-12-20;17(12):e1009982.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Genetic basis for an evolutionary shift from ancestral preaxial to postaxial limb polarity in non-urodele vertebrates
    Authors: Anna Trofka, Bau-Lin Huang, Jianjian Zhu, William F. Heinz, Valentin Magidson, Yuki Shibata et al.
    Current Biology
  22. Prenatal alcohol exposure disrupts Sonic hedgehog pathway and primary cilia genes in the mouse neural tube
    Authors: Karen E. Boschen, Eric W. Fish, Scott E. Parnell
    Reproductive Toxicology
  23. Ciliary and extraciliary Gpr161 pools repress hedgehog signaling in a tissue-specific manner
    Authors: Sun-Hee Hwang, Bandarigoda N Somatilaka, Kevin White, Saikat Mukhopadhyay
    eLife
  24. FBF1 deficiency promotes beiging and healthy expansion of white adipose tissue.
    Authors: Yingyi Z, Jielu H, Mariana T et al.
    Cell Rep.
  25. Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKI gamma
    Authors: Chuan Chen, Qingwen Xu, Yuxia Zhang, Brian A. Davies, Yan Huang, David J. Katzmann et al.
    Science Advances
  26. Centriolar Protein C2cd3 Is Required for Craniofacial Development
    Authors: Ching-Fang Chang, Kari M. Brown, Yanfen Yang, Samantha A. Brugmann
    Frontiers in Cell and Developmental Biology
  27. Time-resolved proteomics profiling of the ciliary Hedgehog response
    Authors: Elena A. May, Marian Kalocsay, Inès Galtier D’Auriac, Patrick S. Schuster, Steven P. Gygi, Maxence V. Nachury et al.
    Journal of Cell Biology
  28. Roles for ELMOD2 and Rootletin in ciliogenesis
    Authors: Rachel E. Turn, Joshua Linnert, Eduardo D. Gigante, Uwe Wolfrum, Tamara Caspary, Richard A. Kahn
    Molecular Biology of the Cell
  29. Activation of Cilia-Independent Hedgehog/GLI1 Signaling as a Novel Concept for Neuroblastoma Therapy
    Authors: A Koeniger, A Brichkina, I Nee, L Dempwolff, A Hupfer, I Galperin, F Finkernage, A Nist, T Stiewe, T Adhikary, W Diederich, M Lauth
    Cancers, 2021-04-15;13(8):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. Myogenesis modelled by human pluripotent stem cells: a multi‐omic study of Duchenne myopathy early onset
    Authors: Virginie Mournetas, Emmanuelle Massouridès, Jean‐Baptiste Dupont, Etienne Kornobis, Hélène Polvèche, Margot Jarrige et al.
    Journal of Cachexia, Sarcopenia and Muscle
  31. Gli3 utilizes Hand2 to synergistically regulate tissue-specific transcriptional networks
    Authors: KH Elliott, X Chen, J Salomone, P Chaturvedi, PA Schultz, SK Balchand, JD Servetas, A Zuniga, R Zeller, B Gebelein, MT Weirauch, KA Peterson, SA Brugmann
    Elife, 2020-10-02;9(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. A Shh/Gli-driven three-node timer motif controls temporal identity and fate of neural stem cells
    Authors: JM Dias, Z Alekseenko, A Jeggari, M Boareto, J Vollmer, M Kozhevniko, H Wang, MP Matise, A Alexeyenko, D Iber, J Ericson
    Science Advances, 2020-09-16;6(38):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation, Western Blot
  33. Ankmy2 Prevents Smoothened-Independent Hyperactivation of the Hedgehog Pathway via Cilia-Regulated Adenylyl Cyclase Signaling
    Authors: Bandarigoda Nipunika Somatilaka, Sun-Hee Hwang, Vivek Reddy Palicharla, Kevin Andrew White, Hemant Badgandi, John Michael Shelton et al.
    Developmental Cell
  34. A transient role of the ciliary gene Inpp5e in controlling direct versus indirect neurogenesis in cortical development
    Authors: Kerstin Hasenpusch-Theil, Christine Laclef, Matt Colligan, Eamon Fitzgerald, Katherine Howe, Emily Carroll et al.
    eLife
  35. The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis
    Authors: Saishu Yoshida, Katsuhiko Aoki, Ken Fujiwara, Takashi Nakakura, Akira Kawamura, Kohji Yamada et al.
    eLife
  36. Downregulation of GLI3 Expression Mediates Chemotherapy Resistance in Acute Myeloid Leukemia
    Authors: Fabian Freisleben, Lena Behrmann, Vanessa Thaden, Jana Muschhammer, Carsten Bokemeyer, Walter Fiedler et al.
    International Journal of Molecular Sciences
  37. Bicyclic Imidazolium Inhibitors of Gli Transcription Factor Activity
    Authors: Marisa E. Hom, Alison E. Ondrus, Tomoyo Sakata‐Kato, Paul G. Rack, James K. Chen
    ChemMedChem
  38. AP-1 controls the p11-dependent antidepressant response
    Authors: RU Chottekala, S Kalik, J Gresack, A Ayala, M Gao, W Wang, S Meller, A Aly, A Schaefer, P Greengard
    Mol. Psychiatry, 2020-05-21;0(0):.
    Species: C. elegans
    Sample Types: Whole Tissue
    Applications: IHC
  39. TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating
    Authors: H Yan, C Chen, H Chen, H Hong, Y Huang, K Ling, J Hu, Q Wei
    Nat Commun, 2020-05-04;11(1):2196.
    Species: C. elegans
    Sample Types: Whole Tissue
    Applications: IHC
  40. Development of the Proximal-Anterior Skeletal Elements in the Mouse Hindlimb Is Regulated by a Transcriptional and Signaling Network Controlled by Sall4
    Authors: Katherine Q Chen, Naoyuki Tahara, Aaron Anderson, Hiroko Kawakami, Sho Kawakami, Ryuichi Nishinakamura et al.
    Genetics
  41. Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration
    Authors: Y Kim, J Lee, M Seppala, MT Cobourne, SH Kim
    Nat Commun, 2020-04-24;11(1):1994.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  42. ARL13B regulates Sonic hedgehog signaling from outside primary cilia
    Authors: Eduardo D Gigante, Megan R Taylor, Anna A Ivanova, Richard A Kahn, Tamara Caspary
    eLife
  43. NRF2 negatively regulates primary ciliogenesis and hedgehog signaling
    Authors: P Liu, M Dodson, D Fang, E Chapman, DD Zhang
    PLoS Biol., 2020-02-13;18(2):e3000620.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  44. The formation of the thumb requires direct modulation of Gli3 transcription by Hoxa13
    Authors: MF Bastida, R Pérez-Góme, A Trofka, J Zhu, A Rada-Igles, R Sheth, HS Stadler, S Mackem, MA Ros
    Proc. Natl. Acad. Sci. U.S.A., 2020-01-02;0(0):.
    Species: Mouse
    Sample Types: Tissue Lysate
    Applications: Western Blot
  45. The ciliary phosphatidylinositol phosphatase Inpp5e plays positive and negative regulatory roles in Shh signaling
    Authors: Sandii Constable, Alyssa B. Long, Katharine A. Floyd, Stéphane Schurmans, Tamara Caspary
    Development
  46. Gli3 regulates vomeronasal neurogenesis, olfactory ensheathing cell formation and GnRH-1 neuronal migration
    Authors: EZM Taroc, A Naik, JM Lin, NB Peterson, DL Keefe, E Genis, G Fuchs, R Balasubram, PE Forni
    J. Neurosci., 2019-11-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  47. NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling
    Authors: A Martin-Hur, R Martin-Mor, N Robledinos, R Blanco, I Palacios-B, I Lastres-Be, A Cuadrado, FR Garcia-Gon
    Sci Rep, 2019-09-25;9(1):13896.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  48. Phosphorylation of Ci/Gli by Fused Family Kinases Promotes Hedgehog Signaling
    Authors: Yuhong Han, Bing Wang, Yong Suk Cho, Jian Zhu, Jiang Wu, Yongbin Chen et al.
    Developmental Cell
  49. ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of beta TrCP
    Authors: Francesca Bufalieri, Paola Infante, Flavia Bernardi, Miriam Caimano, Paolo Romania, Marta Moretti et al.
    Nature Communications
  50. Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification
    Authors: Heejin Nam, Shin Jeon, Hyejin An, Jaeyoung Yoo, Hyo-Jong Lee, Soo-Kyung Lee et al.
    eLife
  51. Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning
    Authors: P Ma, NN Song, Y Li, Q Zhang, L Zhang, L Zhang, Q Kong, L Ma, X Yang, B Ren, C Li, X Zhao, Y Li, Y Xu, X Gao, YQ Ding, B Mao
    Cell Rep, 2019-07-09;28(2):541-553.e4.
    Species: Human, Mouse
    Sample Types: Cell Fractions, Cell Lysates
    Applications: Western Blot
  52. Unexpected Activities in Regulating Ciliation Contribute to Off-target Effects of Targeted Drugs
    Authors: Anna A. Kiseleva, Vladislav A. Korobeynikov, Anna S. Nikonova, Peishan Zhang, Petr Makhov, Alexander Y. Deneka et al.
    Clinical Cancer Research
  53. Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer
    Authors: Christina R Adams, Htet Htwe Htwe, Timothy Marsh, Aprilgate L Wang, Megan L Montoya, Lakshmipriya Subbaraj et al.
    eLife
  54. Gli3 controls the onset of cortical neurogenesis by regulating the radial glial cell cycle through Cdk6 expression
    Authors: Kerstin Hasenpusch-Theil, Stephen West, Alexandra Kelman, Zrinko Kozic, Sophie Horrocks, Andrew P. McMahon et al.
    Development
  55. Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules
    Authors: K He, X Ma, T Xu, Y Li, A Hodge, Q Zhang, J Torline, Y Huang, J Zhao, K Ling, J Hu
    Nat Commun, 2018-08-17;9(1):3310.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  56. Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling
    Authors: SR Hong, CL Wang, YS Huang, YC Chang, YC Chang, GV Pusapati, CY Lin, N Hsu, HC Cheng, YC Chiang, WE Huang, NC Shaner, R Rohatgi, T Inoue, YC Lin
    Nat Commun, 2018-04-30;9(1):1732.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  57. The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts
    Authors: A Struchtrup, A Wiegering, B Stork, U Rüther, C Gerhardt
    Autophagy, 2018-02-21;0(0):1-47.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  58. TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms
    Authors: T Rosengren, LJ Larsen, LB Pedersen, ST Christense, LB Møller
    Cell. Mol. Life Sci., 2018-02-02;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  59. WDR11‐mediated Hedgehog signalling defects underlie a new ciliopathy related to Kallmann syndrome
    Authors: Yeon‐Joo Kim, Daniel PS Osborn, Ji‐Young Lee, Masatake Araki, Kimi Araki, Timothy Mohun et al.
    EMBO reports
  60. CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog
    Authors: Ganesh V. Pusapati, Jennifer H. Kong, Bhaven B. Patel, Arunkumar Krishnan, Andreas Sagner, Maia Kinnebrew et al.
    Developmental Cell
  61. Regulation of Calvarial Osteogenesis by Concomitant De-repression of GLI3 and Activation of IHH Targets
    Authors: LK Veistinen, T Mustonen, MR Hasan, M Takatalo, Y Kobayashi, DA Kesper, A Vortkamp, DP Rice
    Front Physiol, 2017-12-19;8(0):1036.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  62. A novel missense variant in the GLI3 zinc finger domain in a family with digital anomalies
    Authors: J. Aaron Crapster, Louanne Hudgins, James K. Chen, Natalia Gomez-Ospina
    American Journal of Medical Genetics Part A
  63. EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling
    Authors: VJ Guen, TE Chavarria, C Kröger, X Ye, RA Weinberg, JA Lees
    Proc. Natl. Acad. Sci. U.S.A., 2017-11-20;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  64. Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction
    Authors: D Hasegawa, H Ochiai-Shi, S Onodera, T Nakamura, A Saito, T Onda, K Watanabe, K Nishimura, M Ohtaka, M Nakanishi, K Kosaki, A Yamaguchi, T Shibahara, T Azuma
    PLoS ONE, 2017-10-31;12(10):e0186879.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  65. Inhibition of Ciliogenesis Promotes Hedgehog Signaling, Tumorigenesis, and Metastasis in Breast Cancer
    Authors: Nadia B. Hassounah, Martha Nunez, Colleen Fordyce, Denise Roe, Ray Nagle, Thomas Bunch et al.
    Molecular Cancer Research
  66. Differential requirement of SUFU in tissue development discovered in a hypomorphic mouse model
    Authors: Maria A. Hoelzl, Karin Heby-Henricson, Marco Gerling, José M. Dias, Raoul V. Kuiper, Cornelius Trünkle et al.
    Developmental Biology
  67. APC sets the Wnt tone necessary for cerebral cortical progenitor development
    Authors: Naoki Nakagawa, Jingjun Li, Keiko Yabuno-Nakagawa, Tae-Yeon Eom, Martis Cowles, Tavien Mapp et al.
    Genes & Development
  68. Suppressor of Fused Restraint of Hedgehog Activity Level is Critical for Osteogenic Proliferation and Differentiation during Calvarial Bone Development
    Authors: J Li, Y Cui, J Xu, Q Wang, X Yang, Y Li, X Zhang, M Qiu, Z Zhang, Z Zhang
    J. Biol. Chem., 2017-08-09;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  69. Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system
    Authors: Y Han, Y Xiong, X Shi, J Wu, Y Zhao, J Jiang
    PLoS Biol., 2017-08-04;15(8):e2002063.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  70. Sonic Hedgehog Activates Phospholipase A2 to Enhance Smoothened Ciliary Translocation
    Authors: AM Arensdorf, ME Dillard, JM Menke, MW Frank, CO Rock, SK Ogden
    Cell Rep, 2017-06-06;19(10):2074-2087.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  71. Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord
    Authors: H Cai, A Liu
    Dev. Biol., 2017-04-12;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  72. Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision
    Authors: Siew Cheng Phua, Shuhei Chiba, Masako Suzuki, Emily Su, Elle C Roberson, Ganesh V Pusapati et al.
    Cell
  73. Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9
    Sci Rep, 2016-12-21;6(0):39608.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  74. Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling
    Proc. Natl. Acad. Sci. U.S.A, 2016-12-05;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  75. Craniofacial Ciliopathies Reveal Specific Requirements for GLI Proteins during Development of the Facial Midline
    PLoS Genet., 2016-11-01;12(11):e1006351.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  76. Understanding Mechanisms of GLI-Mediated Transcription during Craniofacial Development and Disease Using the Ciliopathic Mutant, talpid2
    Authors: Ya-Ting Chang, Praneet Chaturvedi, Elizabeth N. Schock, Samantha A. Brugmann
    Frontiers in Physiology
  77. Cholesterol activates the G-protein coupled receptor Smoothened to promote morphogenetic signaling
    Elife, 2016-10-05;5(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  78. Mechanism of inhibition of the tumor suppressor Patched by Sonic Hedgehog
    Proc Natl Acad Sci USA, 2016-09-19;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  79. Gata6-Dependent GLI3 Repressor Function is Essential in Anterior Limb Progenitor Cells for Proper Limb Development
    Authors: Shinichi Hayashi, Ryutaro Akiyama, Julia Wong, Naoyuki Tahara, Hiroko Kawakami, Yasuhiko Kawakami
    PLOS Genetics
  80. Centrosomal protein CP110 controls maturation of the mother centriole during cilia biogenesis
    Authors: Sharda Prasad Yadav, Neel Kamal Sharma, Chunqiao Liu, Lijin Dong, Tiansen Li, Anand Swaroop
    Development
  81. Members of the Rusc protein family interact with Sufu and inhibit vertebrate Hedgehog signaling
    Authors: Zhigang Jin, Tyler Schwend, Jia Fu, Zehua Bao, Jing Liang, Huimin Zhao et al.
    Development
  82. T-box3 is a ciliary protein and regulates stability of the Gli3 transcription factor to control digit number
    Authors: U Emechebe, P Kumar P, JM Rozenberg, B Moore, A Firment, T Mirshahi, AM Moon
    Elife, 2016-04-05;5(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  83. Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development
    Authors: Sohinee Bhattacharyya, Mark A Rainey, Priyanka Arya, Bhopal C. Mohapatra, Insha Mushtaq, Samikshan Dutta et al.
    Scientific Reports
  84. Suppressor of Fused Is Required for Determining Digit Number and Identity via Gli3/Fgfs/Gremlin
    Authors: Jianying Li, Qihui Wang, Ying Cui, Xueqin Yang, Yan Li, Xiaoyun Zhang et al.
    PLOS ONE
  85. The Eya1 Phosphatase Promotes Shh Signaling during Hindbrain Development and Oncogenesis
    Authors: Adriana Eisner, Maria F. Pazyra-Murphy, Ershela Durresi, Pengcheng Zhou, Xuesong Zhao, Emily C. Chadwick et al.
    Developmental Cell
  86. T396I mutation of mouse Sufu reduces the stability and activity of Gli3 repressor.
    Authors: Makino S, Zhulyn O, Mo R, Puviindran V, Zhang X, Murata T, Fukumura R, Ishitsuka Y, Kotaki H, Matsumaru D, Ishii S, Hui C, Gondo Y
    PLoS ONE, 2015-03-11;10(3):e0119455.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  87. Bifurcating action of Smoothened in Hedgehog signaling is mediated by Dlg5.
    Authors: Chong Y, Mann R, Zhao C, Kato M, Beachy P
    Genes Dev, 2015-02-01;29(3):262-76.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  88. Sufu and Kif7 in limb patterning and development.
    Authors: Zhulyn O, Hui C
    Dev Dyn, 2015-01-24;244(3):468-78.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  89. The Hedgehog Pathway Effector Smoothened Exhibits Signaling Competency in the Absence of Ciliary Accumulation
    Authors: Chih-Wei Fan, Baozhi Chen, Irene Franco, Jianming Lu, Heping Shi, Shuguang Wei et al.
    Chemistry & Biology
  90. Murine Joubert syndrome reveals Hedgehog signaling defects as a potential therapeutic target for nephronophthisis.
    Authors: Hynes, Ann Mari, Giles, Rachel H, Srivastava, Shalabh, Eley, Lorraine, Whitehead, Jennifer, Danilenko, Marina, Raman, Shreya, Slaats, Gisela G, Colville, John G, Ajzenberg, Henry, Kroes, Hester Y, Thelwall, Peter E, Simmons, Nicholas, Miles, Colin G, Sayer, John A
    Proc Natl Acad Sci U S A, 2014-06-19;111(27):9893-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  91. A switch from low to high Shh activity regulates establishment of limb progenitors and signaling centers.
    Authors: Zhulyn O, Li D, Deimling S, Vakili N, Mo R, Puviindran V, Chen M, Chuang P, Hopyan S, Hui C
    Dev Cell, 2014-04-10;29(2):241-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  92. Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators.
    Authors: Liu Y, Tsai I, Morleo M, Oh E, Leitch C, Massa F, Lee B, Parker D, Finley D, Zaghloul N, Franco B, Katsanis N
    J Clin Invest, 2014-04-01;124(5):2059-70.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  93. GATA6 is a crucial regulator of Shh in the limb bud.
    Authors: Kozhemyakina, Elena, Ionescu, Andreia, Lassar, Andrew B
    PLoS Genet, 2014-01-09;10(1):e1004072.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  94. Gli3 regulation of myogenesis is necessary for ischemia-induced angiogenesis.
    Authors: Renault M, Vandierdonck S, Chapouly C, Yu Y, Qin G, Metras A, Couffinhal T, Losordo D, Yao Q, Reynaud A, Jaspard-Vinassa B, Belloc I, Desgranges C, Gadeau A
    Circ Res, 2013-09-17;113(10):1148-58.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  95. Regulation and function of immunosuppressive molecule human leukocyte antigen G5 in human bone tissue.
    Authors: Deschaseaux F, Gaillard J, Langonne A, Chauveau C, Naji A, Bouacida A, Rosset P, Heymann D, De Pinieux G, Rouas-Freiss N, Sensebe L
    FASEB J, 2013-04-16;27(8):2977-87.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  96. The Ciliary Protein Ftm Is Required for Ventricular Wall and Septal Development
    Authors: Christoph Gerhardt, Johanna M. Lier, Stefanie Kuschel, Ulrich Rüther
    PLoS ONE
  97. The hedgehog system machinery controls transforming growth factor-beta-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis.
    Authors: Cigna N, Farrokhi Moshai E, Brayer S, Marchal-Somme J, Wemeau-Stervinou L, Fabre A, Mal H, Leseche G, Dehoux M, Soler P, Crestani B, Mailleux A
    Am J Pathol, 2012-09-29;181(6):2126-37.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  98. Prevention of premature fusion of calvarial suture in GLI-Kruppel family member 3 (Gli3)-deficient mice by removing one allele of Runt-related transcription factor 2 (Runx2).
    Authors: Tanimoto Y, Veistinen L, Alakurtti K
    J. Biol. Chem., 2012-04-30;287(25):21429-38.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  99. The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins.
    Authors: Humke EW, Dorn KV, Milenkovic L, Scott MP, Rohatgi R
    Genes Dev., 2010-04-01;24(7):670-82.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  100. Gastritis promotes an activated bone marrow-derived mesenchymal stem cell with a phenotype reminiscent of a cancer-promoting cell.
    Authors: Donnelly J, Engevik A, Engevik M, Schumacher M, Xiao C, Yang L, Worrell R, Zavros Y
    Dig Dis Sci, 2013-11-08;59(3):569-82.
  101. Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling.
    Authors: Nachtergaele S, Whalen DM, Mydock LK et al.
    Elife.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse GLI-3 Antibody

Average Rating: 5 (Based on 2 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse GLI-3 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse GLI-3 Antibody
By Anonymous on 05/22/2019
Application: WB Sample Tested: Cell Lysates Species: Mouse
Western Blot Human/Mouse GLI-3 Antibody AF3690
Human/Mouse GLI‑3 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Mouse embryo cell lysate Species: Mouse

Specificity: Specific
Sensitivity: Sensitive
Buffer: TBST
Dilution: 1/1000

Western Blot Human/Mouse GLI‑3 Antibody AF3690