Human/Mouse Glucagon NorthernLights™ NL557-conjugated Antibody

Glucagon in beta TC-6 Mouse Cell Line. Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon NorthernLights™-557 Conjugated Monoclonal Antibody (Catalog # NL1249R) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Glucagon in U2OS cells (Positive) & RT‑4 cells (Negative). Glucagon was detected in immersion fixed U2OS human osteosarcoma cells (Positive) & absent in RT‑4 human urinary bladder transitional cell papilloma (Negative) using Mouse Anti-Human/Mouse Glucagon NorthernLights™ NL557-conjugated Monoclonal Antibody (Catalog # NL1249R) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Nucleolus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.