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Human/Mouse HIF-1 alpha/HIF1A PE-conjugated Antibody

Catalog #: IC1935P
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC1935P
Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry.
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Citations (15)
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Human/Mouse HIF-1 alpha/HIF1A PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Recognizes human and mouse HIF-1 alpha in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 241812
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human HIF-1 alpha
Arg575-Asn826
Accession # Q16665.1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of HIF-1a antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line treated with CoCl2was stained with Mouse Anti-Human/Mouse HIF-1a PE-conjugated Monoclonal Antibody (Catalog # IC1935P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HIF-1 alpha/HIF1A

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Long Name
Hypoxia Inducible Factor 1 Subunit Alpha
Entrez Gene IDs
3091 (Human); 15251 (Mouse); 29560 (Rat)
Alternate Names
AINT; anti-HIF-1 alpha; anti-HIF1A; ARNT interacting protein; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF1 alpha; HIF-1 alpha; HIF1; HIF1A; HIF-1a; HIF-1alpha; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8

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Citations for Human/Mouse HIF-1 alpha/HIF1A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. CD34+/CD45-dim stem cell mobilization by hyperbaric oxygen - Changes with oxygen dosage.
    Authors: Heyboer M, Milovanova TN, Wojcik S et al.
    Stem Cell Res
  2. Inhibition of the mitochondrial pyruvate carrier simultaneously mitigates hyperinflammation and hyperglycemia in COVID-19
    Authors: Bibo Zhu, Xiaoqin Wei, Harish Narasimhan, Wei Qian, Ruixuan Zhang, In Su Cheon et al.
    Science Immunology
  3. Obesity enhances antiviral immunity in the genital mucosa through a microbiota-mediated effect on gammadelta T�cells
    Authors: JH Park, I Kang, HC Kim, Y Lee, SK Lee, HK Lee
    Cell Reports, 2022-11-08;41(6):111594.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. ID2 and HIF-1 alpha collaborate to protect quiescent hematopoietic stem cells from activation, differentiation, and exhaustion
    Authors: Brad L. Jakubison, Tanmoy Sarkar, Kristbjorn O. Gudmundsson, Shweta Singh, Lei Sun, Holly M. Morris et al.
    Journal of Clinical Investigation
  5. Loss of ATF4 leads to functional aging-like attrition of adult hematopoietic stem cells
    Authors: Y Sun, X Lin, B Liu, Y Zhang, W Li, S Zhang, F He, H Tian, X Zhu, X Liu, J Wu, J Cai, M Li
    Science Advances, 2021-12-22;7(52):eabj6877.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Uncoupling of macrophage inflammation from self-renewal modulates host recovery from respiratory viral infection
    Authors: Bibo Zhu, Yue Wu, Su Huang, Ruixuan Zhang, Young Min Son, Chaofan Li et al.
    Immunity
  7. CD74 is a regulator of hematopoietic stem cell maintenance
    Authors: S Becker-Her, M Rozenberg, C Hillel-Kar, N Gil-Yarom, MP Kramer, A Barak, L Sever, K David, L Radomir, H Lewinsky, M Levi, G Friedlande, R Bucala, A Peled, I Shachar
    PloS Biology, 2021-03-04;19(3):e3001121.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Epithelial-mesenchymal transition and metastatic ability of CD133+ colorectal cancer stem-like cells under hypoxia
    Authors: M Okada, K Kawai, H Sonoda, H Shiratori, J Kishikawa, H Nagata, H Nozawa, K Sasaki, M Kaneko, K Murono, S Emoto, Y Iida, H Ishii, Y Yokoyama, H Anzai, K Hasegawa, S Ishihara
    Oncol Lett, 2020-11-09;21(1):19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Elevated Glucose Levels Favor SARS-CoV-2 Infection and Monocyte Response through a HIF-1 alpha /Glycolysis-Dependent Axis
    Authors: Ana Campos Codo, Gustavo Gastão Davanzo, Lauar de Brito Monteiro, Gabriela Fabiano de Souza, Stéfanie Primon Muraro, João Victor Virgilio-da-Silva et al.
    Cell Metabolism
  10. Lactate released by inflammatory bone marrow neutrophils induces their mobilization via endothelial GPR81 signaling
    Authors: E Khatib-Mas, S Bhattachar, H Massalha, A Biram, K Golan, O Kollet, A Kumari, F Avemaria, E Petrovich-, S Gur-Cohen, T Itkin, I Brandenbur, A Spiegel, Z Shulman, Z Gerhart-Hi, S Itzkovitz, M Gunzer, S Offermanns, R Alon, A Ariel, T Lapidot
    Nat Commun, 2020-07-15;11(1):3547.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Silibinin down-regulates PD-L1 expression in nasopharyngeal carcinoma by interfering with tumor cell glycolytic metabolism
    Authors: LS Sellam, R Zappasodi, F Chettibi, D Djennaoui, N Yahi-Ait M, ZC Amir-Tidad, C Touil-Bouk, W Ouahioune, JD Wolchok, T Merghoub, M Bourouba
    Arch. Biochem. Biophys., 2020-07-15;690(0):108479.
    Species: Human
    Sample Types: Cells
    Applications: Flow Cytometry
  12. Impaired enolase 1 glycolytic activity restrains effector functions of tumor-infiltrating CD8 + T cells
    Authors: Lelisa F. Gemta, Peter J. Siska, Marin E. Nelson, Xia Gao, Xiaojing Liu, Jason W. Locasale et al.
    Science Immunology
  13. Induction of HIF-1? by HIV-1 Infection in CD4+ T Cells Promotes Viral Replication and Drives Extracellular Vesicle-Mediated Inflammation
    Authors: G Duette, P Pereyra Ge, J Rubione, PS Perez, AL Landay, SM Crowe, Z Liao, KW Witwer, MP Holgado, J Salido, J Geffner, O Sued, CS Palmer, M Ostrowski
    MBio, 2018-09-11;9(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. SN-38 Acts as a Radiosensitizer for Colorectal Cancer by Inhibiting the Radiation-induced Up-regulation of HIF-1 alpha
    Authors: TAKAYUKI OKUNO, KAZUSHIGE KAWAI, KEISUKE HATA, KOJI MURONO, SHIGENOBU EMOTO, MANABU KANEKO et al.
    Anticancer Research
  15. Glucocorticoid receptor promotes the function of myeloid-derived suppressor cells by suppressing HIF1?-dependent glycolysis
    Authors: Y Lu, H Liu, Y Bi, H Yang, Y Li, J Wang, Z Zhang, Y Wang, C Li, A Jia, L Han, Y Hu, Y Zhao, R Wang, G Liu
    Cell. Mol. Immunol, 2017-03-13;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot

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