Human/Mouse HMGA1B Antibody Summary
Met1-Glu96
Accession # P17096
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse HMGA1B by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HMGA1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5956) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HMGA1B at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HMGA1B
HMGA1B (High mobility group A1, isoform B; also HMG-Y) is a 10‑11 kDa member of the HMGA family of proteins. It is ubiquitously expressed, and occurs principally in tumor and embryonic tissue. HMGA1B is a nonhistone architectural protein that binds to AT-rich DNA sequences. It participates in both gene repression and activation by changing DNA conformation, and is known to regulate miRNA as well. Human HMGA1B is 96 amino acids (aa) in length and contains three A-T hook DNA-binding domains (aa 21‑31; 42‑52; 67‑78). It is constitutively phosphorylated, and may undergo acetylation. There are three potential splice variants. All three show the same 11 aa insertion after Pro34. In addition, one form shows an additional 56 aa substitution for aa 55‑96, while a second shows a 14 aa substitution for aa 80‑96. Full-length human HMGA1B shares 97% aa identity with mouse HMGA1B.
Product Datasheets
Citation for Human/Mouse HMGA1B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids
Authors: Nikoli? MZ, Caritg O, Jeng Q et al.
Elife
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