Human/Mouse IL-12 R beta 2 APC-conjugated Antibody Summary
Cys28-Asn622
Accession # Q99665
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑12 R beta 2 in Human TH1 cells by Flow Cytometry. Human CD4+peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 1 ug/mL plate-bound Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100), 10 ng/mL Recombinant Human IL-12 (Catalog # 219-IL), and 10 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 5 days to induce Th1 cell development, followed by restimulation with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin for 2 to 3 hours, were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and Mouse Anti-Human/Mouse IL-12 R beta 2 APC-conjugated Monoclonal Antibody (Catalog # FAB1959A). Quadrant markers were set based on control antibody staining using Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-12 R beta 2
Interleukin 12 (IL-12), the founding member of the IL-12 family of heterodimeric cytokines, is composed of two disulfide-linked 35 kDa and 40 kDa subunits. The 35 kDa subunit (p35) is a alpha -helical protein homologous to IL-6 and G-CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also a subunit of IL-23. IL-12 is an essential mediator of cellular-immunity that induces T cells and natural-killer cells to produce IFN-gamma. It is also required for the expansion and activation Th1 cells (1, 2).
The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of the IL-12 Receptor beta 1 (IL-12 R beta 1) and IL-12 Receptor beta 2 (IL‑12 R beta 2) subunits. IL-12 R beta 1 is a 100 kDa protein that is also a subunit of the IL-23 receptor complex. It binds IL-12/IL-23 p40 and is associated with Tyk2. IL‑12 R beta 2 is a 130 kDa protein that interacts with p35 and is associated with Jak2. Both receptor subunits are type I membrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 2 cDNA encodes a 862 amino acid (aa) protein with a putative 27 aa signal peptide that is cleaved to generate the mature protein with a 595 aa extracellular domain, a 24 aa transmembrane domain and a 216 aa cytoplasmic region. Human and mouse IL‑12 R beta 2 share 68% amino acid sequence identity. Whereas IL-12 R beta 1 expression has been detected in activated T cells, NK cells and B cells, the expression of IL‑12 R beta 2 is more restricted. Among T cells, IL-12 R beta 2 is absent on naive T cells. Activation of T cells via TCR up‑regulates IL‑12 R beta 2 expression on human Th1 but not Th2 cells (1‑4).
- Trinchieri, G. et al. (2003) Immunity 19:641.
- Brombacher, F. et al. (2003) Trends in Immunol. 23:207.
- Trinchieri, G. (2003) Nature Reviews Immunol. 3:133.
- Rogge, L. et al. (1997) J. Exp. Med. 185:825.
Product Datasheets
Citations for Human/Mouse IL-12 R beta 2 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Tet2 deficiency drives liver microbiome dysbiosis triggering Tc1 cell autoimmune hepatitis
Authors: Surya P. Pandey, Mackenzie J. Bender, Alex C. McPherson, Catherine M. Phelps, Luzmariel Medina Sanchez, Mohit Rana et al.
Cell Host & Microbe
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Systemic frequencies of T helper 1 and T helper 17 cells in patients with age-related macular degeneration: A case-control study
Authors: Amardeep Singh, Yousif Subhi, Marie Krogh Nielsen, Mads Krüger Falk, Sara Maj Hyldig Matzen, Finn Sellebjerg et al.
Scientific Reports
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Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity
Authors: Lars Fugger
Sci Transl Med, 2016-11-02;8(363):363ra149.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Multiplex immunoassay characterization and species comparison of inflammation in acute and non-acute ischemic infarcts in human and mouse brain tissue
Acta Neuropathol Commun, 2016-09-06;4(1):100.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
AKT hyperactivation confers a Th1 phenotype in thymic Treg cells deficient in TGF-beta receptor II signaling.
Authors: Liu Y, Xu Y, Sun J, Ma A, Zhang F, Xia S, Xu G, Liu Y
Eur J Immunol, 2013-12-02;44(2):521-32.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Modulation of cytokine receptors by IL-2 broadly regulates differentiation into helper T cell lineages.
Authors: Liao W, Lin JX, Wang L, Li P, Leonard WJ
Nat. Immunol., 2011-04-24;12(6):551-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Single low-dose rHuIL-12 safely triggers multilineage hematopoietic and immune-mediated effects
Authors: Mamata S Gokhale, Vladimir Vainstein, Jamie Tom, Simmy Thomas, Chris E Lawrence, Zoya Gluzman-Poltorak et al.
Experimental Hematology & Oncology
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mouse CD4 T cells were activated with anti-CD3 and anti-CD28 antibodies in presence of IL-2 for 5 days.
