Human/Mouse IL-5 Alexa Fluor® 700-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
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Preparation and Storage
Background: IL-5
IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.
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Citation for Human/Mouse IL-5 Alexa Fluor® 700-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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A booster dose enhances immunogenicity of the COVID-19 vaccine candidate ChAdOx1 nCoV-19 in aged mice
Authors: Alyssa Silva-Cayetano, William S. Foster, Silvia Innocentin, Sandra Belij-Rammerstorfer, Alexandra J. Spencer, Oliver T. Burton et al.
Med (N Y)
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Splenocytes from C57BL/6 and Foxp3-deficient Scurfy mice were stimulated for 4 hours with phorbol 12,13-dibutyrate (500ng/ml), ionomycin (750ng/ml) and brefeldin A (2ug/ml). Cells were stained with fixable viability dye, then fixed with 2% formalin, permeabilized, and stained overnight for cytokines and gating markers (CD3, CD4).
Foxp3-deficient mice were chosen as a positive control because they are highly Th2-biased. IL-5 is not normally present in C57BL/6 CD4s in any great quantity. Another appropriate positive control would be ILC2 (e.g., gut lamina propria leukocytes).
Unstimulated cells that were otherwise treated identically served as a negative control.
Flow diagrams are shown gating on viable CD3+CD4+ T cells.
