Human/Mouse IRF4 Catalytic Subunit Antibody

Name: Human/Mouse IRF4 Catalytic Subunit Antibody
Brand: R&D Systems
SKU: MAB5525

Catalog #: MAB5525 Datasheet / COA / SDS

An anti-human IRF4 alternative is available.
AF5525

Discontinued Product

MAB5525 has been discontinued.

View all IRF4 products.

Detection of Human/Mouse IRF4 Catalytic Subunit by Western Blot.

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Human/Mouse IRF4 Catalytic Subunit Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human and mouse IRF4 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 503215

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IRF4
Glu130-Glu451
Accession # Q15306

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse IRF4 Catalytic Subunit by Western Blot. Western blot shows nuclear extracts of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse IRF4 Catalytic Subunit Monoclonal Antibody (Catalog # MAB5525) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRF4 Catalytic Subunit at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Immunohistochemistry

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IRF4 in Human Tonsil. IRF4 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human/Mouse IRF4 Catalytic Subunit Monoclonal Antibody (Catalog # MAB5525) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF4

Interferon Regulatory Factor 4 (IRF4), also known as MUM1 and LSIRF, is a 51 kDa lymphocyte-restricted transcription factor. It is required for immunoglobulin class switching and terminal differentiation of B cells. IRF4 is overexpressed in multiple myeloma and cooperates with Myc in an autoregulatory loop. In T cells, IRF4 is required for the production of IL-4. IRF4 contains an N-terminal DNA binding domain that is homologous to that in other IRF proteins. Within the C-terminal domain (aa 130‑451), human IRF4 shares 90% aa sequence identity with mouse and rat IRF4. Alternate splicing may generate isoforms with N-terminal, C-terminal, or internal deletions.

Long Name

Interferon Regulatory Factor 4

Entrez Gene IDs

3662 (Human); 16364 (Mouse); 291100 (Rat)

Alternate Names

interferon regulatory factor 4; IRF4; IRF-4; LSIRF; LSIRFMUM1; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM1; NF-EM5