Human/Mouse Leukotriene A4 Hydrolase/
LTA4H Antibody
Human/Mouse Leukotriene A4 Hydrolase/
LTA4H Antibody Summary
Pro2-Asp611
Accession # P09960
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Leukotriene A4 Hydrolase/LTA4H by Western Blot. Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, NCI‑N87 human gastric carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse Leukotriene A4 Hydrolase/LTA4H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4008) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Leukotriene A4 Hydrolase/LTA4H at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Leukotriene A4 Hydrolase/LTA4H
Leukotrienes are a family of lipid mediators important in a variety of allergic and inflammatory reactions. Synthesized by leukocytes, these molecules are divided into two classes, the spasmogenic cysteinyl leukotrienes and LTB4, a classical chemoattractant (1). Encoded by the LTA4H gene, Leukotriene A4 Hydrolase catalyzes the conversion of unstable epoxide LTA4 to LTB4, which is the final and committed step in LTB4 biosynthesis. As a bifunctional zinc metalloenzyme, LTA4H also acts as an arginyl aminopeptidase (2). LTA4H is a drug target for anti-inflammation, and for cancer prevention and therapy (1, 3).
The mature chain of human LTA4H consists of 610 amino acids (residues 2‑611). It is highly specific for LTA4, which also covalently modifies and inhibits the enzyme. The aminopeptidase activity is enhanced by monovalent anions (1). R&D Systems rhLTA4H corresponds to the mature chain, and is characterized by its arginyl aminopeptidase activity.
- Haeggstrom, J.Z. (2006) J. Biol. Chem. 279:50639.
- Orning, L. et al. (1994) J. Biol. Chem. 269:11269.
- Chen, X. et al. (2004) Curr. Cancer Drug Targets 4:267.
Product Datasheets
Citation for Human/Mouse Leukotriene A4 Hydrolase/
LTA4H Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Protein folding stress potentiates NLRP1 and CARD8 inflammasome activation
Authors: EL Orth-He, HC Huang, SD Rao, Q Wang, Q Chen, CM O'Mara, AJ Chui, M Saoi, AR Griswold, A Bhattachar, DP Ball, JR Cross, DA Bachovchin
Cell Reports, 2023-01-16;0(0):111965.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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