Human/Mouse Lysine (K)-specific Demethylase 4A/KDM4A Antibody

Catalog #: AF6434 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6434
AF6434-SP

Detection of Human and Mouse Lysine (K)‑specific Demethylase 4A/KDM4A by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Lysine (K)-specific Demethylase 4A/KDM4A Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human KDM4A in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human KDM4C is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human KDM4A
Lys386-Ser514
Accession # AAH02558

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Lysine (K)‑specific Demethylase 4A/KDM4A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, THP-1 human acute monocytic leukemia cell line, and mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Human Lysine (K)-specific Demethylase 4A/KDM4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6434) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lysine (K)-specific Demethylase 4A/KDM4A at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry

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KDM4A in BG01V Human Cell Line. KDM4A was detected in immersion fixed endoderm-differentiated BG01V human embryonic stem cells using Human/Mouse KDM4A Affinity-purified Polyclonal Antibody (Catalog # AF6434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lysine (K)-specific Demethylase 4A/KDM4A

KDM4A (Lysine [K]-specific demethylase 4A; also known as JMJD2A) is a 155-160 kDa member of the JHDM3 histone demethylase family of enzymes. It is ubiquitously expressed and interacts with the histone deacetylase proteins HDAC-1, -2, and -3. KDM4A specifically demethylates trimethylated lysines on histone H3 at Lys9 and Lys36. Transcriptional repression occurs when it interacts with NCOR1. Human KDM4A is 1064 amino acids (aa) in length (SwissProt #:O75164). It contains one jumonji N domain (aa 13-55), a catalytic jumonji C domain (aa 175-291), two PHD zinc finger regions (aa 709-855), and two TUDOR methylated-histone binding domains (aa 897-1011). Over aa 386-514, human KDM4A shares 93% amino acid sequence identity with mouse KDM4A.

Entrez Gene IDs

9682 (Human); 230674 (Mouse); 313539 (Rat)

Alternate Names

EC 1.14.11; EC 1.14.11.-; JHDM3A; JHDM3AJMJD2; JmjC domain-containing histone demethylation protein 3A; JMJD2; JMJD2A; JMJD2Alysine-specific demethylase 4A; jumonji domain containing 2; jumonji domain containing 2A; Jumonji domain-containing protein 2A; KDM4A; KIAA0677jumonji C domain-containing histone demethylase 3A; Lysine (K)specific Demethylase 4A; Lysine (K)-specific Demethylase 4A