Human/Mouse MCPIP1 Antibody Summary
Asp426-Glu599
Accession # Q5D1E8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse MCPIP1 by Western Blot. Western blot shows lysates of untreated (-) HeLa human cervical epithelial carcinoma cell line and RAW 264.7 mouse monocyte/macrophage cell line and THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 1 µg/mL LPS for 24 hours or 10 µg/mL LPS for 3 hours, respectively. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MCPIP1 Monoclonal Antibody (Catalog # MAB7875) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MCPIP1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MCPIP1
Human MCP-induced protein (MCPIP), also known as ZC3H12A, is an approximately 66 kDa intracellular protein that contains one RNAse domain (aa 134-290), one zinc finger (aa 301-324), and a proline-rich region (aa 458-536). Within aa 426-599, human MCPIP shares approximately 79% aa sequence identity with mouse and rat MCPIP. Its expression is induced by inflammatory stimulation and cellular stress. It acts to dampen inflammatory responses by promoting the degradation of proinflammatory cytokine mRNAs, inhibiting NFkB activation, and antagonizing TLR signaling. MCPIP exhibits deubiquitinase activity and inhibits the biogenesis of miRNA. It also enhances inflammation-induced angiogenesis, osteocyte and adipocyte differentiation, and the cell death of cardiac myocytes.
Product Datasheets
Citations for Human/Mouse MCPIP1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Disrupting Roquin-1 interaction with Regnase-1 induces autoimmunity and enhances antitumor responses
Authors: G Behrens, SL Edelmann, T Raj, N Kronbeck, T Monecke, E Davydova, EH Wong, L Kifinger, F Giesert, ME Kirmaier, C Hohn, LS de Jonge, MG Pisfil, M Fu, S Theurich, S Feske, N Kawakami, W Wurst, D Niessing, V Heissmeyer
Nature Immunology, 2021-11-22;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
RTEC-intrinsic IL-17-driven inflammatory circuit amplifies antibody-induced glomerulonephritis and is constrained by Regnase-1
Authors: DD Li, R Bechara, K Ramani, CV Jawale, Y Li, JK Kolls, SL Gaffen, PS Biswas
JCI Insight, 2021-07-08;6(13):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
CARD19, the protein formerly known as BinCARD, is a mitochondrial protein that does not regulate Bcl10-dependent NF-kappa B activation after TCR engagement.
Authors: Kariana E. Rios, Anuj K. Kashyap, Sean K. Maynard, Michael Washington, Suman Paul, Brian C. Schaefer
Cellular Immunology
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MALT1 Phosphorylation Controls Activation of T Lymphocytes and Survival of ABC-DLBCL Tumor Cells
Authors: T Gehring, T Erdmann, M Rahm, C Gra beta, A Flatley, TJ O'Neill, S Woods, I Meininger, O Karayel, K Kutzner, M Grau, H Shinohara, K Lammens, R Feederle, SM Hauck, G Lenz, D Krappmann
Cell Rep, 2019-10-22;29(4):873-888.e10.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Bcl10-controlled Malt1 paracaspase activity is key for the immune suppressive function of regulatory T cells
Authors: M Rosenbaum, A Gewies, K Pechloff, C Heuser, T Engleitner, T Gehring, L Hartjes, S Krebs, D Krappmann, M Kriegsmann, W Weichert, R Rad, C Kurts, J Ruland
Nat Commun, 2019-05-28;10(1):2352.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Aggregation of Human Trophoblast Cells into Three-Dimensional Culture System Enhances Anti-Inflammatory Characteristics through Cytoskeleton Regulation
Authors: K Seno, Y Munakata, M Sano, R Kawahara-M, H Takahashi, A Ohkuchi, H Iwata, T Kuwayama, K Shirasuna
Int J Mol Sci, 2018-08-08;19(8):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Malt1 self-cleavage is critical for regulatory T cell homeostasis and anti-tumor immunity in mice
Authors: M Baens, R Stirparo, Y Lampi, D Verbeke, R Vandepoel, J Cools, P Marynen, CE de Bock, S Bornschein
Eur. J. Immunol., 2018-08-07;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Autocleavage of the paracaspase MALT1 at Arg-781 attenuates NF-?B signaling and regulates the growth of activated B-cell like diffuse large B-cell lymphoma cells
Authors: CH Wu, YH Yang, MR Chen, CH Tsai, AL Cheng, SL Doong
PLoS ONE, 2018-06-28;13(6):e0199779.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Primary T cells were activated with PMA/ionomycin for the time points as shown. Cell lysates were harvested in a NP40-based lysis buffer. 8% SDS-PAGE, transferred to a nitrocellulose membrane by semi-dry transfer machine. Blocked by 5% milk for 30 mins. Antibody dilution was used at 1:500. 680nm Donkey anti-mouse was used as secondary antibody (1:30,000). Image captured by Licor Odyssey. The arrow shows the full length Regnase-1, and the rectangle shows the cleaved product upon TCR stimulation.
