Human/Mouse MYF-5 APC-conjugated Antibody

Name: Human/Mouse MYF-5 APC-conjugated Antibody
Brand: R&D Systems
SKU: IC4027A

Catalog #: IC4027A Datasheet / COA / SDS

Discontinued Product

IC4027A has been discontinued.

View all MYF-5 products.

Detection of MYF‑5 in C2C12 Mouse Cell Line by Flow Cytometry.

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Human/Mouse MYF-5 APC-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human MYF-5 in direct ELISAs and mouse MYF-5 in flow cytometry.

Source

Monoclonal Mouse IgG_2A Clone # 593128

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MYF-5
Met1-Leu255
Accession # P13349

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of MYF‑5 antibody in C2C12 Mouse Cell Line antibody by Flow Cytometry.

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Detection of MYF‑5 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Mouse Anti-Human/Mouse MYF-5 APC-conjugated Monoclonal Antibody (Catalog # IC4027A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MYF-5

MYF-5 (Myogenic Factor 5), also known as bHLHc2, is a 37-39 kDa transcriptional activator that belongs to the bHLH superfamily of myogenic regulatory factors. It is found in embryonic skeletal muscle progenitors and neurons, and is induced by SHH and Wnt1. Expression of MYF-5 protein prompts nonmuscle cells to adopt a muscle precursor phenotype. Notably, neurons appear to block MYF-5 accumulation, thus preserving their unique phenotype. MYF-5 forms heterodimers with multiple molecules. When complexed to I-mfa, MYF-5 is retained in the cytoplasm and is inactive; when complexed to E12, MYF-5 is transcriptionally active. Phosphorylation regulates MYF-5 stability. Human MYF-5 is 255 amino acids (aa) in length. It contains one myogenic basic region (aa 1-83) followed by an HLH motif (aa 96-135). Full-length human MYF-5 (aa 1-255) shares 89% aa sequence identity with mouse MYF-5.

Long Name

Myogenic Regulatory Factor 5

Entrez Gene IDs

4617 (Human); 17877 (Mouse); 299766 (Rat)

Alternate Names

bHLHc2; bHLHc2Class C basic helix-loop-helix protein 2; MYF5; MYF-5; myogenic factor 5