Human/Mouse Myocardin Antibody Summary
Met97-Leu290
Accession # Q8IZQ8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Myocardin by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human Myocardin Monoclonal Antibody (Catalog # MAB4028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myocardin at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Mouse Myocardin by Western Blot Laminin-111 but not laminin-alpha 4 blocking antibody affects pericyte differentiation. (a) Immunoblots show that laminin-111 blockage (Ln Ab) significantly enhances the expression of PDGFR beta, SMA, and SM22-alpha, but not myocardin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14b. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=5–6). (b) Immunoblots show that laminin-alpha 4 blockage (Anti-Ln alpha 4) does not change the expression of PDGFR beta, SMA, SM22-alpha, or myocardinin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14c. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=3). Data are shown as mean ± sd. *p<0.05 versus the Ctrs by student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24583950), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Myocardin by Western Blot Astrocytic laminin mediates pericyte differentiation via integrin alpha 2. (a) Immunoblots show that integrin alpha 2 blockage (ITGA2) but not integrin beta 1 blockage significantly increases the expression of PDGFR beta, SMA, and SM22-alpha, but not myocardin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14d. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=6). (b) Schematic diagram of shRNA designed to target ITGA2 mRNA. (c) Immunoblot analysis shows that all three ITGA2-specific shRNAs (#1–3) dramatically reduce ITGA2 at protein level and ITGA2-specific shRNA-3 (#1) is the most efficient one. Full blots of ITGA2 and actin are shown in Supplementary Figure 14e. Scramble shRNA was used as a Ctr. (d) Immunoblot analysis shows that transduction of pericytes with lenti-shRNA-1 (#1) significantly enhances the expression of PDGFR beta, SMA, and SM22-alpha, but does not affect myocardin level. Full blots of these proteins are shown in Supplementary Figure 14f. Scramble shRNA was used as a Ctr. All bands were normalized to actin (n=4–5). Data are shown as mean ± sd. *p<0.05 versus the Ctrs by student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24583950), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Myocardin by Western Blot Laminin-111 but not laminin-alpha 4 blocking antibody affects pericyte differentiation. (a) Immunoblots show that laminin-111 blockage (Ln Ab) significantly enhances the expression of PDGFR beta, SMA, and SM22-alpha, but not myocardin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14b. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=5–6). (b) Immunoblots show that laminin-alpha 4 blockage (Anti-Ln alpha 4) does not change the expression of PDGFR beta, SMA, SM22-alpha, or myocardinin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14c. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=3). Data are shown as mean ± sd. *p<0.05 versus the Ctrs by student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24583950), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Myocardin by Western Blot Astrocytic laminin mediates pericyte differentiation via integrin alpha 2. (a) Immunoblots show that integrin alpha 2 blockage (ITGA2) but not integrin beta 1 blockage significantly increases the expression of PDGFR beta, SMA, and SM22-alpha, but not myocardin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14d. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=6). (b) Schematic diagram of shRNA designed to target ITGA2 mRNA. (c) Immunoblot analysis shows that all three ITGA2-specific shRNAs (#1–3) dramatically reduce ITGA2 at protein level and ITGA2-specific shRNA-3 (#1) is the most efficient one. Full blots of ITGA2 and actin are shown in Supplementary Figure 14e. Scramble shRNA was used as a Ctr. (d) Immunoblot analysis shows that transduction of pericytes with lenti-shRNA-1 (#1) significantly enhances the expression of PDGFR beta, SMA, and SM22-alpha, but does not affect myocardin level. Full blots of these proteins are shown in Supplementary Figure 14f. Scramble shRNA was used as a Ctr. All bands were normalized to actin (n=4–5). Data are shown as mean ± sd. *p<0.05 versus the Ctrs by student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24583950), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Myocardin
Myocardin (MYOCD) is a transcriptional co-activator necessary for differentiation of smooth muscle cells. MYOCD functions by binding the transcription factor Serum Response Factor (SRF) and stimulating smooth muscle cell-specific gene expression.
Product Datasheets
Citations for Human/Mouse Myocardin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
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Smooth muscle cell-specific FoxM1 controls hypoxia-induced pulmonary hypertension
Authors: J Dai, Q Zhou, H Tang, T Chen, J Li, P Raychaudhu, JX Yuan, G Zhou
Cell. Signal., 2018-08-06;0(0):.
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Myocardin Is Involved in Mesothelial–Mesenchymal Transition of Human Pleural Mesothelial Cells
Authors: Torry Tucker, Yoshikazu Tsukasaki, Tsuyoshi Sakai, Shinya Mitsuhashi, Satoshi Komatsu, Ann Jeffers et al.
American Journal of Respiratory Cell and Molecular Biology
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Vascular Klotho Deficiency Potentiates the Development of Human Artery Calcification and Mediates Resistance to Fibroblast Growth Factor 23
Authors: Kenneth Lim, Tzong-Shi Lu, Guerman Molostvov, Christina Lee, F.T. Lam, Daniel Zehnder et al.
Circulation
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Disruption of miR-29 Leads to Aberrant Differentiation of Smooth Muscle Cells Selectively Associated with Distal Lung Vasculature
Authors: Leah Cushing, Stefan Costinean, Wei Xu, Zhihua Jiang, Lindsey Madden, Pingping Kuang et al.
PLOS Genetics
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The mitochondrial protease ClpP is a druggable target that controls VSMC phenotype by a SIRT1-dependent mechanism
Authors: Paredes, F;Williams, HC;Liu, X;Holden, C;Bogan, B;Wang, Y;Crotty, KM;Yeligar, SM;Elorza, AA;Lin, Z;Rezvan, A;San Martin, A;
Redox biology
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Alpha-enolase regulates the malignant phenotype of pulmonary artery smooth muscle cells via the AMPK-Akt pathway
Authors: J Dai, Q Zhou, J Chen, ML Rexius-Hal, J Rehman, G Zhou
Nat Commun, 2018-09-21;9(1):3850.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: IHC, Western Blot -
Downregulation of Insulin Receptor Substrate-1 During Hyperglycemia Induces Vascular Smooth Muscle Cell Dedifferentiation
Authors: Gang Xi
J. Biol. Chem, 2016-12-21;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
miR-17/20 Controls Prolyl Hydroxylase 2 (PHD2)/Hypoxia-Inducible Factor 1 (HIF1) to Regulate Pulmonary Artery Smooth Muscle Cell Proliferation.
Authors: Tianji Chen, Qiyuan Zhou, Haiyang Tang, Melike Bozkanat, Jason X-J Yuan, J Usha Raj, Guofei Zhou
Journal of the American Heart Association, 2016-12-05;0(0):2047-9980.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Co-activation of nuclear factor-kappaB and myocardin/serum response factor conveys the hypertrophy signal of high insulin levels in cardiac myoblasts.
Authors: Madonna R, Geng Y, Bolli R, Rokosh G, Ferdinandy P, Patterson C, De Caterina R
J Biol Chem, 2014-05-22;289(28):19585-98.
Species: Canine, Mouse
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Western Blot -
Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity.
Authors: Yao Y, Chen Z, Norris E, Strickland S
Nat Commun, 2014-03-03;5(0):3413.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Akt1 mediates alpha-smooth muscle actin expression and myofibroblast differentiation via myocardin and serum response factor.
Authors: Abdalla M, Goc A, Segar L, Somanath P
J Biol Chem, 2013-10-08;288(46):33483-93.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Transplantation of mesenchymal cells rejuvenated by the overexpression of telomerase and myocardin promotes revascularization and tissue repair in a murine model of hindlimb ischemia.
Authors: Madonna R, Taylor D, Geng Y, De Caterina R, Shelat H, Perin E, Willerson J
Circ Res, 2013-06-18;113(7):902-14.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Induction of intracellular heat-shock protein 72 prevents the development of vascular smooth muscle cell calcification.
Authors: Lu, Tzong-Sh, Lim, Kenneth, Molostvov, Guerman, Yang, Yun-Chun, Yiao, Szu-Yu, Zehnder, Daniel, Hsiao, Li-Li
Cardiovasc Res, 2012-08-29;96(3):524-32.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Hypertension impairs myocardin function: a novel mechanism facilitating arterial remodelling.
Authors: Pfisterer, Larissa, Feldner, Anja, Hecker, Markus, Korff, Thomas
Cardiovasc Res, 2012-07-26;96(1):120-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Down-regulation of Kruppel-like Factor-4 (KLF4) by MicroRNA-143/145 Is Critical for Modulation of Vascular Smooth Muscle Cell Phenotype by Transforming Growth Factor-{beta} and Bone Morphogenetic Protein 4.
Authors: Davis-Dusenbery BN, Chan MC, Reno KE, Weisman AS, Layne MD, Lagna G, Hata A
J. Biol. Chem., 2011-06-13;286(32):28097-110.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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