Human/Mouse NETO2 Antibody

Catalog #: AF3859 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3859
AF3859-SP

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Human/Mouse NETO2 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse NETO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant human NETO1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NETO2 isoform 1
Ile23-Lys345
Accession # Q8NC67

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human NETO2
Recombinant Mouse NETO2

Immunoprecipitation

1-2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunoprecipitation

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Immunoprecipitation of Mouse NETO2. NETO2 was immunoprecipitated from lysates (500 µg) of mouse brain tissue following incubation with 1‑2 µg/mL Goat Anti-Human/Mouse NETO2 Antigen Affinity-purified Polyclonal Antibody for 8 hours at 2‑8 °C. NETO2-antibody complexes were absorbed using Protein G (Sigma). Immunoprecipitated NETO2 was detected by Western blot using 1 µg/mL Goat Anti-Human/Mouse NETO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3859). View our recommended buffer recipes for immunoprecipitation.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NETO2

NETO2 is a neurospecific type I transmembrane protein belonging to the NETO family of proteins that share homology with the axon guidance receptors, neuropilin and the developmentally important metalloproteinase Tolloid/BMP-1. NETO proteins have two CUB domains, an LDL-receptor class A domain in their extracellular region and a conserved FXNPXY-like domain in the cytoplasmic region. A splice variant of NETO2 that lacks the CUB domains and most of the LDL class A domain has been reported. It is likely that like NETO1, NETO2 may be a synaptic protein with neurological function. Human and mouse NETO2 share 98% amino acid sequence homology in their extracellular domains.

Long Name

Neuropilin [NRP] And Tolloid [TLL]-like 2

Entrez Gene IDs

81831 (Human); 74513 (Mouse); 307757 (Rat)

Alternate Names

Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor classA domains protein 2; BTCL2; FLJ10430; FLJ90456; NEOT2; NEOT2FLJ14724; NETO2; neuropilin (NRP) and tolloid (TLL)-like 2; neuropilin and tolloid-like protein 2