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Human/Mouse Neuroplastin 65 Antibody

Catalog #: AF5360
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5360
AF5360-SP
Neuroplastin 65 in Human Brain.
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Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Neuroplastin 65 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Neuroplastin 65 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Neuroplastin 55 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Neuroplastin 65
Gln29-His336
Accession # NP_036560
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Neuroplastin 65 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Neuroplastin 65 in Human Brain. Neuroplastin 65 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 3 µg/mL Goat Anti-Human/Mouse Neuroplastin 65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5360) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human/Mouse Neuroplastin 65 antibody by Western Blot. View Larger

Detection of Human/Mouse Neuroplastin 65 by Western Blot. Western blot shows lysates of human brain and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Neuroplastin 65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5360) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Neuroplastin 65 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroplastin 65

Neuroplastin (NPTN; also stromal cell-derived receptor 1 and Np55) is a 52-57 kDa member of the Ig-superfamily. It is widely expressed and likely serves as a cell adhesion molecule in multiple tissues. Human Neuroplastin is 282 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains two Ig-like domains (aa 32-119 and 122-213) and a 38 aa cytoplasmic region (aa 245-282). There is a brain-enriched 65 kDa form (Np65) that negatively regulates LTP in the hippocampus. It contains an N terminal 116 aa Ig-like domain-containing insert after Asn30 and interacts homotypically. Notably, human and rodent Np65 expression patterns are different in brain, with rodent Np65 not predominating in cerebellum. Over aa 29-336, human Np65 is 95% aa identical to mouse Np65.

Entrez Gene IDs
27020 (Human); 56064 (Rat)
Alternate Names
GP55;GP65;np55;np65;NPTN;SDFR1;SDR1; Neuroplastin 65; Np65

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Citations for Human/Mouse Neuroplastin 65 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Disfunction of dorsal raphe nucleus-hippocampus serotonergic-HTR3 transmission results in anxiety phenotype of Neuroplastin 65-deficient mice
    Authors: Cheng, J;Chen, L;Zheng, YN;Liu, J;Zhang, L;Zhang, XM;Huang, L;Yuan, QL;
    Acta pharmacologica Sinica
    Species: Mouse, Transgenic Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: Immunohistochemistry, Western Blot
  2. Neuroplastin 65 deficiency reduces amyloid plaque formation and cognitive deficits in an Alzheimer's disease mouse model
    Authors: Wu, DD;Cheng, J;Zheng, YN;Liu, YT;Hou, SX;Liu, LF;Huang, L;Yuan, QL;
    Frontiers in cellular neuroscience
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Protein composition of axonal dopamine release sites in the striatum
    Authors: Kershberg L, Banerjee A, Kaeser PS.
    eLife
  4. In vivo visualization and molecular targeting of the cardiac conduction system
    Authors: William R. Goodyer, Benjamin M. Beyersdorf, Lauren Duan, Nynke S. van den Berg, Sruthi Mantri, Francisco X. Galdos et al.
    Journal of Clinical Investigation
  5. Disarranged neuroplastin environment upon aging and chronic stress recovery in female Sprague Dawley rats
    Authors: Marta Balog, Senka Blažetić, Vedrana Ivić, Irena Labak, Bartosz Krajnik, Raquel Marin et al.
    European Journal of Neuroscience
  6. Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing
    Authors: Sherylanne Newton, Fanbo Kong, Adam J. Carlton, Carlos Aguilar, Andrew Parker, Gemma F. Codner et al.
    PLOS Genetics
  7. Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts
    Authors: Ilic K, Lin X, Malci A et al.
    International Journal of Molecular Sciences
  8. Hippocampal expression of cell-adhesion glycoprotein neuroplastin is altered in Alzheimer's disease
    Authors: Katarina Ilic, Kristina Mlinac-Jerkovic, Natasa Jovanov-Milosevic, Goran Simic, Nikola Habek, Nenad Bogdanovic et al.
    Journal of Cellular and Molecular Medicine
  9. Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function
    J Neurosci, 2016-08-31;36(35):9201-16.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. Absence of Neuroplastin-65 Affects Synaptogenesis in Mouse Inner Hair Cells and Causes Profound Hearing Loss
    Authors: Leanne Carrott, Michael R. Bowl, Carlos Aguilar, Stuart L. Johnson, Lauren Chessum, Melissa West et al.
    The Journal of Neuroscience
  11. Neuroplastin-65 and a mimetic peptide derived from its homophilic binding site modulate neuritogenesis and neuronal plasticity.
    Authors: Owczarek S, Soroka V, Kiryushko D, Larsen MH, Yuan Q, Sandi C, Berezin V, Bock E
    J. Neurochem., 2011-05-13;117(6):984-94.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC

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