Human/Mouse NLRP3/NALP3 Alexa Fluor® 488-conjugated Antibody Summary
Met1-Arg153
Accession # Q8R4B8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of NLRP3/NALP3 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse NLRP3/NALP3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7578G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
View Larger
Detection of NLRP3/NALP3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human/Mouse NLRP3/NALP3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7578G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NLRP3/NALP3
NLRP3, also known as NALP3, CIAS1, PYPAF or Cryopyrin, is a cytosolic ~120 kDa member of the NLRP family of proteins expressed in leukocytes, especially neutrophils. As a component of the inflammasome, NLRP3 activates caspases 1 and 5. Defects in NLRP3 may cause FCAS1, CINCA, or Muckle-Wells autoinflammatory syndromes. Mouse NLRP3 contains an N-terminal Pyrin domain (aa 1-91) followed by a Nacht region (aa 216-532), and seven LRRs (aa 739-988). Within the sequence used as an immunogen, mouse NLRP3 shares 78% and 93% aa identity with human and rat NLRP3, respectively. Alternate splicing of mouse NLRP3 generates additional isoforms that lack either LRR2 and 3, LRR 6 and 7, or LRR4-9.
Product Datasheets
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
Citations for Human/Mouse NLRP3/NALP3 Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
Prostaglandin D2 is involved in the regulation of inflammatory response in Staphylococcus aureus-infected mice macrophages
Authors: Bao, H;Gong, Z;Zhao, J;Ren, P;Yu, Z;Su, N;Gong, L;Mao, W;Liu, B;Zhang, S;Yang, Y;Cao, J;
International immunopharmacology
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Besides TLR2 and TLR4, NLRP3 is also involved in regulating Escherichia coli infection-induced inflammatory responses in mice
Authors: Shen, Y;Gong, Z;Zhang, S;Cao, J;Mao, W;Yao, Y;Zhao, J;Li, Q;Liu, K;Liu, B;Feng, S;
International immunopharmacology
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Term and Preterm Birth Initiation Is Associated with the Macrophages Shifting to M1 Polarization in Gestational Tissues in Mice
Authors: Y Shan, S Shen, J Long, Z Tang, C Wu, X Ni
Biology, 2022-12-04;11(12):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse NLRP3/NALP3 Alexa Fluor® 488-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse NLRP3/NALP3 Alexa Fluor® 488-conjugated Antibody and earn rewards!
Have you used Human/Mouse NLRP3/NALP3 Alexa Fluor® 488-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
