Human/Mouse Norrin Antibody

Name: Human/Mouse Norrin Antibody
Brand: R&D Systems
SKU: MAB3014
Rating: 5 (1 reviews)

Catalog #: MAB3014
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3014
MAB3014-SP

All Norrin Antibodies

Product Details

Citations (2)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Norrin Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Norrin in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse Norrin is observed.

Source

Monoclonal Mouse IgG₁ Clone # 343711

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Norrin
Lys25-Ser133
Accession # Q00604

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Norrin (Catalog # 3014-NR) under reducing conditions only
Recombinant Mouse Norrin (Catalog # 3497-NR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Norrin

Norrin, also known as Norrie Disease Protein, is a secreted protein that has a C-terminal cysteine-knot motif. Although Norrin is unrelated to Wnt family proteins, it is a Frizzled-4-specific ligand that activates the canonical Wnt pathway through Frizzled-4 and LRP5 (LDL receptor-related protein). Mutations in Norrin are associated with Norrie disease and X-linked familial exudative vitroretinopathy. The amino acid sequence of human Norrin is 100% and 94% identical to rhesus macaque and mouse Norrin, respectively.

Entrez Gene IDs

4693 (Human)

Alternate Names

EVR2; exudative vitreoretinopathy 2 (X-linked); FEVR; ND; NDP; Norrie disease (pseudoglioma); Norrie disease protein; Norrin; X-linked exudative vitreoretinopathy 2 protein

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse Norrin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Cholinergic neural activity directs retinal layer-specific angiogenesis and blood retinal barrier formation
Authors: GA Weiner, SH Shah, CM Angelopoul, AB Bartakova, RS Pulido, A Murphy, E Nudleman, R Daneman, JL Goldberg
Nat Commun, 2019-06-06;10(1):2477.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Proteomics analysis identifies new markers associated with capillary cerebral amyloid angiopathy in Alzheimer's disease
Authors: DC Hondius, KN Eigenhuis, THJ Morrema, RC van der Sc, P van Nierop, M Bugiani, KW Li, JJM Hoozemans, AB Smit, AJM Rozemuller
Acta Neuropathol Commun, 2018-06-04;6(1):46.
Species: Human
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot