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Human/Mouse Nurr1/NGFI-B beta /NR4A2 Antibody

Catalog #: AF2156
19 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2156
AF2156-SP
Nurr1/NGFI‑B beta /NR4A2 in HeLa Human Cell Line.
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Product Details
Citations (19)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Nurr1/NGFI-B beta /NR4A2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Nurr1/NGFI‑B beta /NR4A2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Nurr1/NGFI‑B beta /NR4A2
Val332-Lys558
Accession # Q06219
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Nurr1/NGFI‑B beta /NR4A2
Immunohistochemistry
5-15 µg/mL
Immersion fixed differentiated dopamenergic nuerons from D3 mouse embryonic stem cells
Immunocytochemistry
5-15 µg/mL
Immersion fixed HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Nurr1/NGFI‑B beta /NR4A2 in HeLa Human Cell Line. Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry View Larger

Nurr1/NGFI‑B beta /NR4A2 in Mouse Brain (Hippocampus). Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed paraffin-embedded sections of mouse brain (hippocampus) using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nurr1/NGFI-B beta/NR4A2

Nurr1 belongs to the nerve growth factor-induced clone B subfamily of nuclear receptor superfamily and is designated NR4A2 in the Unified Nomenclature System for NRs. Nurr1 can function as a ligand-independent transcription factor. Nurr1 also heterodimerizes with retinoid X receptors (RXRs) to function as ligand-regulated transcription factor. Nurr1 is important for specification of dopaminergic neurons that degenerate in Parkinson’s disease. Human and mouse Nurr1 share 99% amino acid sequence homology.

Long Name
Nur-related Factor 1/Nerve Growth Factor Induced B beta/Nuclear Receptor Subfamily 4, Group A, 2
Entrez Gene IDs
4929 (Human); 18227 (Mouse)
Alternate Names
human homolog of; NGFI-B beta; NOT; NR4A2; nuclear receptor subfamily 4, group A, member 2; Nurr1; orphan nuclear receptor NR4A2; Orphan nuclear receptor NURR1; T-cell nuclear receptor NOT; TINUR; transcriptionally inducible nuclear receptor related 1; Transcriptionally-inducible nuclear receptor

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Citations for Human/Mouse Nurr1/NGFI-B beta /NR4A2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. A PBX1 transcriptional network controls dopaminergic neuron development and is impaired in Parkinson's disease
    Authors: Villaescusa JC, Li B, Toledo EM et al.
    EMBO J.
  2. Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells.
    Authors: La Manno G, Gyllborg D et al.
    Cell
  3. A simple and reliable method for claustrum localization across age in mice
    Authors: Shaker, T;Dagpa, GJ;Cattaud, V;Marriott, BA;Sultan, M;Almokdad, M;Jackson, J;
    Molecular brain
  4. Celf4 controls mRNA translation underlying synaptic development in the prenatal mammalian neocortex
    Authors: Salamon I, Park Y, Mi�ki? T et al.
    Nat Commun
  5. Tle4 controls both developmental acquisition and early post-natal maturation of corticothalamic projection neuron identity
    Authors: Galazo, MJ;Sweetser, DA;Macklis, JD;
    Cell reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Pyramidal neurons form active, transient, multilayered circuits perturbed by autism-associated mutations at the inception of neocortex
    Authors: Martin Munz, Arjun Bharioke, Georg Kosche, Verónica Moreno-Juan, Alexandra Brignall, Tiago M. Rodrigues et al.
    Cell
  7. Temporal origin of mouse claustrum and development of its cortical projections
    Authors: Anna Hoerder-Suabedissen, Gabriel Ocana-Santero, Thomas H Draper, Sophie A Scott, Jesse G Kimani, Andrew M Shelton et al.
    Cerebral Cortex
  8. Dopaminergic neurons are not a major Sonic hedgehog ligand source for striatal cholinergic or PV interneurons
    Authors: Flavia Correa Turcato, Elliot Wegman, Tao Lu, Nathan Ferguson, Yu Luo
    iScience
  9. Inhibition of CSPG receptor PTPsigma promotes migration of newly born neuroblasts, axonal sprouting, and recovery from stroke
    Authors: F Luo, J Wang, Z Zhang, Z You, A Bedolla, F Okwubido-W, LF Huang, J Silver, Y Luo
    Oncogene, 2022-07-26;40(4):111137.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Regulation of prefrontal patterning and connectivity by retinoic acid
    Authors: M Shibata, K Pattabiram, B Lorente-Ga, D Andrijevic, SK Kim, N Kaur, SK Muchnik, X Xing, G Santpere, AMM Sousa, N Sestan
    Nature, 2021-10-01;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Quantitative Immunohistochemistry to Measure Regional Expression of Nurr1 in the Brain and the Effect of the Nurr1 Heterozygous Genotype
    Authors: Evangel Kummari, Shirley X. Guo-Ross, Heath S. Partington, Jennifer Makenzie Nutter, Jeffrey B. Eells
    Frontiers in Neuroanatomy
  12. Altered White Matter and Layer VIb Neurons in Heterozygous Disc1 Mutant, a Mouse Model of Schizophrenia
    Authors: Shin-Hwa Tsai, Chih-Yu Tsao, Li-Jen Lee
    Frontiers in Neuroanatomy
  13. Loss of Dmrt5 Affects the Formation of the Subplate and Early Corticogenesis
    Authors: Leslie Ratié, Elodie Desmaris, Fernando García-Moreno, Anna Hoerder-Suabedissen, Alexandra Kelman, Thomas Theil et al.
    Cerebral Cortex
  14. Striatal Nurr1 Facilitates the Dyskinetic State and Exacerbates Levodopa-Induced Dyskinesia in a Rat Model of Parkinson's Disease
    Authors: RC Sellnow, K Steece-Col, F Altwal, IM Sandoval, JH Kordower, TJ Collier, CE Sortwell, AR West, FP Manfredsso
    J. Neurosci., 2020-04-01;40(18):3675-3691.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  15. Evolutionary Gain of Dbx1 Expression Drives Subplate Identity in the Cerebral Cortex
    Authors: Y Arai, AW Cwetsch, E Coppola, S Cipriani, H Nishihara, H Kanki, Y Saillour, B Freret-Hod, A Dutriaux, N Okada, H Okano, C Dehay, J Nardelli, P Gressens, T Shimogori, G D'Onofrio, A Pierani
    Cell Rep, 2019-10-15;29(3):645-658.e5.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  16. Genome Stability by DNA polymerase ? in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development
    Authors: K Onishi, A Uyeda, M Shida, T Hirayama, T Yagi, N Yamamoto, N Sugo
    J. Neurosci., 2017-08-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Extracortical origin of some murine subplate cell populations.
    Authors: Pedraza M, Hoerder-Suabedissen A, Albert-Maestro M, Molnar Z, De Carlos J
    Proc Natl Acad Sci U S A, 2014-04-28;111(23):8613-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. The Corticofugal Neuron-Associated Genes ROBO1, SRGAP1, and CTIP2 Exhibit an Anterior to Posterior Gradient of Expression in Early Fetal Human Neocortex Development
    Authors: Bui Kar Ip, Nadhim Bayatti, Nicholas J. Howard, Susan Lindsay, Gavin J. Clowry
    Cerebral Cortex
  19. Deficiency of the NR4A neuron-derived orphan receptor-1 attenuates neointima formation after vascular injury.
    Authors: Nomiyama T, Zhao Y, Gizard F, Findeisen HM, Heywood EB, Jones KL, Conneely OM, Bruemmer D
    Circulation, 2009-01-19;119(4):577-86.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot

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