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Human/Mouse Oct-3/4 PE-conjugated Antibody

Catalog #: IC1759P
14 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC1759P-100
IC1759P-025

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Detection of Oct‑3/4 in NTERA-2 cells by Flow Cytometry
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Citations (14)
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Human/Mouse Oct-3/4 PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
Source
Monoclonal Rat IgG2B Clone # 240408
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
NTERA-2 human testicular embryonic carcinoma cell line or BG01V human embryonic stem cells and mouse embryonic stem cells treated with Retinoic Acid were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Oct‑3/4 in NTERA-2 cells by Flow Cytometry NTERA-2 cells were stained with Rat Anti-Human/Mouse Oct‑3/4 PE‑conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of Oct-3/4 antibody in BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 PE-conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of Oct-3/4 antibody in Mouse Stem Cells antibody by Flow Cytometry. View Larger

Detection of Oct‑3/4 in Mouse Stem Cells by Flow Cytometry. Mouse embryonic stem cells treated with 5 µM Retinoic Acid for 3 days were stained with Rat Anti-Human/Mouse Oct-3/4 PE-conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Flow Cytometry Detection of Human Oct-3/4 by Flow Cytometry View Larger

Detection of Human Oct-3/4 by Flow Cytometry Immunophenotype of RS-hAFSCs cultured in in different conditions. (A) Flow cytometry to show the expression of OCT4-A, OCT4-B, SSEA4, Tra1-60, and CD24. Isotype control in blue. (B) Relative mean fluorescence intensity (MFI) for the different groups (measured from 10,000 cells and using the same antibody concentration, experimental conditions and cytometer settings for all groups). Data represent mean ± SEM. SEM: standard error of the mean. **P < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28747757), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oct-3/4

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells (1- 3). Oct-3/4 is required to sustain stem cell self-renewal and pluripotency (4). It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

References
  1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.
Long Name
Octamer-binding Protein 3/4
Entrez Gene IDs
5460 (Human); 18999 (Mouse)
Alternate Names
3-Oct; Oct-3/4; OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1; Oct4; Otf3g; Otf4; Pou5f1

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Citations for Human/Mouse Oct-3/4 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Detailed Characterization of Human Induced Pluripotent Stem Cells Manufactured for Therapeutic Applications
    Authors: Behnam Ahmadian Baghbaderani, Adhikarla Syama, Renuka Sivapatham, Ying Pei, Odity Mukherjee, Thomas Fellner et al.
    Stem Cell Reviews and Reports
  2. Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A
    Authors: F Vlahova, KE Hawkins, AM Ranzoni, KL Hau, R Sagar, P Coppi, AL David, J Adjaye, PV Guillot
    Sci Rep, 2019-05-31;9(1):8126.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, ICC, Western Blot
  3. Generation of hiPSTZ16 (ISMMSi003-A) cell line from normal human foreskin fibroblasts
    Authors: M Dejosez, TP Zwaka
    Stem Cell Res, 2017-12-02;26(0):44-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells
    PLoS ONE, 2016-09-08;11(9):e0161229.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Phenotypic diversity of patient-derived melanoma populations in stem cell medium.
    Authors: Sztiller-Sikorska M, Hartman M, Talar B, Jakubowska J, Zalesna I, Czyz M
    Lab Invest, 2015-04-13;95(6):672-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Scalable passaging of adherent human pluripotent stem cells.
    Authors: Nie Y, Walsh P, Clarke D, Rowley J, Fellner T
    PLoS ONE, 2014-01-30;9(1):e88012.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells.
    Authors: Huijbers I, Bin Ali R, Pritchard C, Cozijnsen M, Kwon M, Proost N, Song J, de Vries H, Badhai J, Sutherland K, Krimpenfort P, Michalak E, Jonkers J, Berns A
    EMBO Mol Med, 2014-01-08;6(2):212-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
    Gene Ther, 2011-11-10;19(10):1035-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Human embryonic stem cells with maintenance under a feeder-free and recombinant cytokine-free condition.
    Authors: Nakahara M, Saeki K, Nakamura N
    Cloning Stem Cells, 2009-03-01;11(1):5-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Octamer 4 small interfering RNA results in cancer stem cell-like cell apoptosis.
    Authors: Hu T, Liu S, Breiter DR, Wang F, Tang Y, Sun S
    Cancer Res., 2008-08-15;68(16):6533-40.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Kidney tissue reconstruction by fetal kidney cell transplantation: effect of gestation stage of fetal kidney cells.
    Authors: Kim SS, Gwak SJ, Han J, Park HJ, Park MH, Song KW, Cho SW, Rhee YH, Chung HM, Kim BS
    Stem Cells, 2007-06-01;25(6):1393-401.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. IGF-1 contributes to the expansion of melanoma-initiating cells through an epithelial-mesenchymal transition process
    Authors: Vincent Le Coz, Chaobin Zhu, Aurore Devocelle, Aimé Vazquez, Claude Boucheix, Sandy Azzi et al.
    Oncotarget
  13. Isolation and characterization of renal cancer stem cells from patient-derived xenografts.
    Authors: Hasmim Meriem, Bruno Stefania, Azzi Sandy et al.
    Oncotarget
  14. Efficient and simultaneous generation of hematopoietic and vascular progenitors from human induced pluripotent stem cells
    Authors: Tea Soon Park, Ludovic Zimmerlin, Elias T. Zambidis
    Cytometry Part A

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